The initial pharmaceutical development of an artesunate/amodiaquine oral formulation for the treatment of malaria: a public-private partnership
© Lacaze et al; licensee BioMed Central Ltd. 2011
Received: 21 December 2010
Accepted: 23 May 2011
Published: 23 May 2011
Artemisinin-based combination therapy is currently recommended worldwide for the treatment of uncomplicated malaria. Fixed-dose combinations are preferred as they favour compliance. This paper reports on the initial phases of the pharmaceutical development of an artesunate-amodiaquine (ASAQ) bilayer co-formulation tablet, undertaken following pre-formulation studies by a network of scientists and industrials from institutions of both industrialized and low income countries.
Pharmaceutical development was performed by a research laboratory at the University Bordeaux Segalen, School of Pharmacy, for feasibility and early stability studies of various drug formulations, further transferred to a company specialized in pharmaceutical development, and then provided to another company for clinical batch manufacturing. The work was conducted by a regional public-private not-for-profit network (TropiVal) within a larger Public Private partnership (the FACT project), set up by WHO/TDR, Médecins Sans Frontières and the Drugs for Neglected Disease initiative (DNDi).
The main pharmaceutical goal was to combine in a solid oral form two incompatible active principles while preventing artesunate degradation under tropical conditions. Several options were attempted and failed to provide satisfactory stability results: incorporating artesunate in the external phase of the tablets, adding a pH regulator, alcoholic wet granulation, dry granulation, addition of an hydrophobic agent, tablet manufacturing in controlled conditions. However, long-term stability could be achieved, in experimental batches under GMP conditions, by physical separation of artesunate and amodiaquine in a bilayer co-formulation tablet in alu-alu blisters. Conduction of the workplan was monitored by DNDi.
Collaborations between research and industrial groups greatly accelerated the process of development of the bi-layered ASAQ tablet. Lack of public funding was the main obstacle hampering the development process, and no intellectual property right was claimed. This approach resulted in a rapid technology transfer to the drug company Sanofi-Aventis, finalizing the process of development, registration and WHO pre-qualification of the fixed-dose co-formulation together with DNDi. The bi-layered tablet is made available under the names of Coarsucam® and Artesunate amodiaquine Winthrop®, Sanofi-Aventis. The issue related to the difficulty of public institutions to valorise their participation in such initiative by lack of priority and funding of applied research is discussed.
The World Health Organization (WHO) currently recommends artemisinin-based combination therapy (ACT) for the treatment of uncomplicated Plasmodium falciparum malaria . Virtually all malaria endemic countries have adopted by now an ACT as first-line treatment. Initially only individually formulated compounds were available; subsequently, products were co-blistered. In both cases patient's adherence may be compromised. This called for the development of fixed-dose co-formulations (FDC). However combining two anti-malarial drugs into a single tablet may be challenging, and requires compliance to stringent regulatory requirements.
The first FDC ACT ever registered was artemether plus lumefantrine (Coartem® and Riamet®, Novartis) launched in 2001. Other widely-used combinations at that time were artesunate plus amodiaquine and artesunate plus mefloquine.
The Special Programme for Research and Training in Tropical Diseases of the WHO (TDR) initiated exploratory pharmaceutical work on anti-malarial drug associations based on AS and AQ , under its Resistance And Policy (RAP) taskforce at the University of Bordeaux 2, France local consortium TropiVal  and the University Sains Malaysia.
Target product profile of developed ASAQ formulation
Target product characteristics
AS and AQ
pediatric and adult
based on 
ASAQ low strength 25/67.5 mg (equivalent AQ base) for children
ASAQ high strength 100/270 mg (equivalent AQ base) for older children and adults
rapid disintegration and fast drug release (> 75% in 45 minutes in vitro dissolution test, Pharmacopoeia standards)
compatible with pediatric use: minimal size for facilitating swallowing; a fixed dose combination
compatible with climatic zone IV (tropical conditions): ICH Q1A(R2)
This paper recounts the development process that led to the formulation of ASAQ in bilayer tablets up to the clinical batches used for initial clinical studies performed in Burkina Faso , and subsequently transferred to commercial partners identified by DNDi (Sanofi-Aventis in 2005, Bompart F, Kiechel JR, Sebbag R, Pecoul B: Innovative public-private partnership to maximize the delivery of anti-malarial medicines: lessons learned from the ASAQ Winthrop experience, submitted).
Artesunate (AS) was purchased from Knoll, Switzerland (currently Abbott Laboratories) and amodiaquine hydrochloride (AQH) salt (AQ.2HCl.2H2O) and amodiaquine base (AQb) were obtained from Parke Davis, Senegal and Ipca, India. Dihydroartemisinin (DHA), AS main metabolite, was provided by Knoll. Solvents and buffers were of analytical grade. Tablet excipients were of pharmaceutical grade. The physicochemical properties of AS and AQ were characterized and reported previously .
Artesunate and DHA assay
AS was determined by HPLC with UV detection on Varian ProStar model chromatograph apparatus equipped with Hypersil C4 column, 5 μm, 250 mm × 4.6 mm. Sodium acetate trihydrate 0.05 M/NaOH for pH 5,2/acetonitrile mixture (63/37%V/V) was used as the mobile phase with flow rate of 1.5 ml.min-1. The injection volume was 100 μl and the oven temperature 27°C, UV detector was set to 211 nm. In these conditions, the retention time of AS was 8 minutes, that of DHA of 11 and 16 minutes (the α and β DHA epimers, respectively) and that of artemisinin of 10 minutes. The analysis time was 15 minutes.
AS standard solution (1 mg mL-1 in mobile phase) was diluted in mobile phase to obtain calibration curve (n≥5) for the desired concentration range. This method was validated according to ICH guidelines. Further internal specifications were implemented: RSD of 6 consecutive injections < 2%.
AQ was dosed by spectrophotometry using PERKIN ELMER lambda 20 Spectrophotometer at 342 nm with cell thickness of 1 mm. AQ standard solution (89 mg of amodiaquine hydrochloride completed to 50 mL with 1% HCl) was further diluted ten-fold with a 1% hydrochloric acid solution.
ASAQ tablet preparation and evaluation
From pre-formulation studies  it was clear that some key parameters, namely water or humidity presence, HCl release or AS AQ contact, have to be controlled for suitable stability of obtained tablets. The development was a series of pharmacotechnical manufacturing processes beginning from the simplest one and going towards more complicated ones to overcome the problems raised regarding target product profile. The aim was to obtain a stable ASAQ tablet with the simplest possible technology. First, direct compression was discarded, then granulation step was considered (aqueous, alcoholic and dry), with or without excipients to enhance tablet stability (pH regulator, hydrophobic agent...). The optimized process ended up with the choice of a bilayer ASAQ tablet.
In all monolayer ASAQ tablets, sodium croscarmellose (Acdisol®) was used as diluent/desintegrant, polyvinylpyrrolidone (PVP K30® or K25® 12%/16% aqueous solution) as a binder, colloidal silica (Aerosil® 300) as flowing agent, and magnesium stearate was used as lubricant. The different components were sieved through a 1 mm grate, AQ was sieved through a 250 μm mesh diameter siever). All monolayer tablets were compressed using a rotary SVIAC PR6 tablet press equipped with 2 round 13 mm diameter punches.
Several preparation techniques were used to optimize ASAQ tablets. In tablets prepared by wet granulation, internal phase constituents (AS, AQ and a part of Acdisol® for batch n° 20399, AQ and Acdisol® for batch n° 20454) were mixed in a planetary mixer and moistened with 12% PVP K30® solution and 16%PVPK25® solution respectively. The moist mass was then passed through 1.6 mm mesh of oscillating granulator and dried in fluid air bed dryer at 60°C for 35 minutes. After drying, granulate was screened again (0.80 mm mesh), mixed with external phase constituents (lubricants and 1%w/w of Acdisol® for batch 20399, AS and CaCO3 in addition in batch n° 20454) before compression.
Dry granulation tablets (batches 30411-30439) were prepared using POWTEC RC 100 × 30 compactor equipped with a rotative calibrator for AQ alone compaction. The plates obtained were calibrated through a 0.8 mm screen and then mixed in planetary mixer with other excipients.
Tablet stability and pre-stability studies
For short-term pre-stability studies, tablets were placed in sealed glass vials at 25°C/60% RH and 40°C/75% RH according to ICH guidelines ICH QA1(R2). In the absence of tropical zone ambient storage department, 60 month stability study of bilayer tablets was carried out in CHU Pellegrin hospital official drug storage department, France at 15 to 25°C and ambient humidity. Tablets were packaged in Alu/Alu blisters for optimized humidity protection. Several conditioned aliquots of each tablet batch were analysed for AS, AQ and DHA content at defined times. Regulatory stability studies for ASAQ formulations were further performed by Sanofi-Aventis.
Tablet AS and DHA content determination
10 or 20 tablets were finely powdered in a mortar. An aliquot of 1,4 g was put into a 100 ml volumetric flask, completed after stirring with water/ethanol (40/60%V/V) and filtered through a 0.45 μm filter. 50 μl were injected according to the HPLC AS method described above. AS was quantified by comparison with a test solution of 2 mg/ml in water/ethanol (40/60%V/V). The specifications used were AS content (100 mg or 25 mg) ± 5%w/w (according to ICH specifications) and further internal specifications for DHA content < 3% after 36 months aging (< 1% DHA in short term studies) were applied, as DHA indicated an ongoing AS degradation process.
Tablet AQ content determination
10 or 20 tablets were finely powdered in a mortar. An aliquot of 700 mg was put into a 200 ml volumetric flask, dissolved in 150 ml 0.1N HCl sonicated for 10 minutes and electromagnetically stirred for 20 minutes and completed. The obtained solution was filtered through a 0.45 μm filter, diluted ten-fold in HCl 0.1N and analysed using AQ spectrophotometric method described. AQ was quantified comparing to test solution of 88 mg AQH dissolved in 50 ml 0,1N HCl and diluted ten-fold. The specifications were AQ content (270 mg or 67 mg) ± 5% w/w (ICH stability study specifications).
ASAQ tablet dissolution test
Dissolution studies were carried out in 500 ml and 900 ml of 11.4 g.l-1 sodium acetate and 0.886 g.l-1 acetic acid aqueous solution adjusted to pH 5.5 for low dosage (25 mg AS 67.5 mg AQ) and high dosage (100 mg AS 270 mg AQ) ASAQ tablets respectively, using European pharmacopoeia apparatus 2 (100 rpm, 37°C). At defined times (15, 30, 45, 60, 90 minutes) samples were withdrawn with 10 μm porous prefilter equipped syringes. Drug concentration in sample was determined using previously described HPLC method for AS and DHA and UV spectrophotometric method for AQ and comparing dissolution samples to standard solutions. The specifications were AQ and AS drug release > 75% at 45 minutes.
A direct compression process proved not feasible as AQ compressibility was poor and the high-strength tablets required too large a quantity of excipients for an acceptable tablet size.
Aqueous wet granulation
The initial wet granulation monolayer tablet formulation containing sodium croscarmellose, PVP K30, magnesium stearate and silicone dioxide was found unstable leading to 9% AS loss after 3 months at 40°C/75%RH . When this process was adapted to industrial scale (modification of wet granulation parameters, flowability and compressibility improvement), AS degradation increased and only 83.7 and 79.6% of AS were found for the high and low-strength ASAQ tablets respectively after 2 months at 40°C/75%RH.
Several approaches were considered to avoid previously identified factors that affect AS degradation (such as humidity, released HCl and AQ contact, ): incorporation of AS in the tablet external phase, inclusion of pH regulator, alcoholic wet granulation, dry granulation, increased hydrophobic agents, tablet manufacturing in controlled conditions, physical separation between AS and AQ (bilayer tablets).
AS in external phase
AS was incorporated in the external tablet phase (after the granulation step) in order to avoid direct contact with water during aqueous wet granulation of AQ. Tablets were found stable during two months at 40°C/75%RH, but 17% AS degradation occurred during the third month.
Alcoholic wet granulation
Pharmacotechnical characteristics of ASAQ alcoholic/aqueous granulation tablet formulations with or without binder*
Tablet dosage AS/AQ (mg/mg)
Aqueous + PVP*
Alcoholic + PVP*
Alcoholic without PVP*
Aqueous + PVP*
Alcoholic + PVP*
Alcoholic without PVP*
Mean tablet weight (mg)
Disintegration time (min)
Drugs released after 45 min of dissolution test (AQ%/AS%)
Dry granulation process
Beyond avoiding water contact, which was the first objective of dry granulation studies, this process was particularly suitable for developed formulation, as a small tablet size was required. AS was added in the external phase of tablets and 17% pH regulator content included. Dry granulation tablet stability was largely improved (> 95% AS and 100% AQ after three months at 40°C/75%RH; batch n° 30411, Figure 4) compared to wet granulation tablets (84% AS; batch n° 20454 Figure 3) with the same formulation (containing pH regulator). Nevertheless 3.4% of DHA found in dry granulation tablets indicated ongoing AS degradation.
To further reduce negative water impact on AS stability in ASAQ tablets, higher silicon dioxide percentage (1.9%w/w, contrarily to 0.9%w/w used as flowing agent in initial and all further formulations) was added to AS in external phase to improve its hydrophobic properties. However AS degradation increased in the presence of higher content of silicon dioxide in all tested formulations (e.g. batch n° 30412 vs. 30411) (Figure 4).
Controlled temperature and humidity conditions
To prevent ASAQ tablets from humidity contact, tablets (batch n° 30435 to 30438) were manufactured in controlled conditions (21°C ± 3°C and 32 ± 4%RH). These conditions enhanced AS stability (Figure 4) as AS content was stable after a three-month stability study, and as the DHA content decreased compared to non-controlled conditions (2.2% vs. 3.4% DHA respectively). However, this was considered not enough to warrant further development. Since all the approaches above had failed and since basic quinoline structures (like AQ) as well as the presence of HCl appeared to be involved in AS degradation , the physical separation between the two APIs was the next option to be considered.
ASAQ bilayer tablets
Bilayer ASAQ tablet formulation for high dosage and low dosage ASAQ tablets
Layer 1 (blend no 30154C01 )
high dosage tablet (batch 30154C03)
low dosage tablet (batch 30154C04)
Dry granulated AQH
Sodium Croscarmellose (AcDiSol)
Polyvinylpyrrolidone (PVP K30)
Silicon dioxide (Aerosil 300)
Total layer 1
Layer 2 (blend 30154C03)
Polyvinylpyrrolidone (PVP K30)
Sodium Croscarmellose (AcDiSol)
Silicon dioxide (Aerosil 300)
Total layer 2
Pharmacotechnical properties of various ASAQ monolayer and bilayer tablet formulations
Flowability (s/100 g)
Hausner index (Vo/Vf)
Residual humidity (%)
AS and DHA recovery after 3 months in accelerated aging conditions (40°C/75%RH)
AS content (%)
DHA content (%)
Monolayer ASAQ tablet
Bilayer ASAQ tablet
Drug recovery (%) in ASAQ bilayer tablets after 6 months in various aging conditions.
High dosage tablets
Low dosage tablets
AS is easily degraded by heat and aqueous conditions [9, 10]. The key factors for AS degradation have been identified in pre-formulation studies as the combination of humidity (> 1%w/w) and temperature (≥40°C) on one hand (typically tropical conditions), and the presence of AQ (or another quinoline, e.g. quinine) and/or HCl on the other hand .
To prevent AS degradation several strategies were tried and many proved inadequate:
◦ Formulating AQ in the inner phase and AS in the external phase of tablets.
◦ Reducing residual water presence in the tablets either through wet or dry granulation. For alcoholic wet granulation, handling 95% ethanolic solution at industrial scale was not possible. Replacing wet granulation by dry granulation (AQ powder was not suitable for direct compression) was not sufficient to ensure AS stability and the addition of an hydrophobic agent (silicon dioxide) increased AS degradation, possibly through radical interaction .
◦ Manufacturing in controlled atmospheric conditions still led to > 5% AS tablet content loss after 3 months at 40°C/75%RH.
◦ Adding a pH regulator (CaCO3) improved partly AS stability but did not prevent degradation completely in dry granulation tablets even when combined with controlled atmospheric conditions manufacturing as some DHA was still detected.
◦ Controlled atmosphere conditions manufacturing did not meet cost requirements. Tablet protective coating was not considered as a suitable condition to avoid water contact.
In all the formulations above, residual humidity (see Table 4) in tablets exceeded the 1%w/w limit identified in pre-formulation studies for AS degradation . Instead, experimental batches of bilayer ASAQ tablets, whereby the two active ingredients are physically isolated, met stability and dissolution criteria. Despite an initial water content slightly > 1%w/w (1.5% and 1.3% for AQ and AS granulate respectively) tablet drug content was stable for at least six months in accelerated stability conditions and for 60 months at 25°C/60%RH when packaged in alu/alu blisters (particularly adapted to storage in hot and humid areas like the tropics). In vitro drug release from these tablets was comparable to commercially available single-agent reference products. The disposition in healthy human subjects of the fixed-dose tablets was comparable, though not completely bio-equivalent, to the individually formulated drugs . Furthermore, 60 months stability study of our pilot batch of ASAQ in ambient European conditions augurs well for the long-term stability of the formulation. If these results are confirmed in zone IV conditions, the product shelf life (currently 36 months) could be further extended, which would simplify drug procurement, storage and delivery.
Establishing and running the operations of the FACT network proved challenging but rewarding. The project was funded through different mechanisms: following initial seed funds from WHO/TDR to the Biopharmacy Laboratory of Bordeaux2 University for validation of the pre-formulation , funding was provided by DNDi, the European Commission INCO-DEV programme, MSFand TDR . Most participating institutions donated personnel costs. When the project was passed onto Sanofi-Aventis for registration and commercialization in 2004, the company complemented with the chemistry, manufacturing and control part of the dossier for the product manufactured at industrial scale, which also included pharmaceutical and clinical studies. Overall, the project required five years from the bench to registration. Both the timelines and the costs are comparatively lower by orders of magnitude to the typical R&D process of a new chemical entity conducted by the pharmaceutical industry. In this case, the cost of failure in the early phases of pharmaceutical development was absorbed by the initial funders. Both, DNDi, Sanofi-Aventis and TDR have continued to invest in phase 4 and implementation studies.
The product was developed as public good and together with DNDi, ELLIPSE Pharmaceuticals decided to forsake the intellectual property rights associated to the process. The rights of producing and commercializing the product were initially transferred in 2005 by DNDi to Sanofi-Aventis with a time-limited exclusivity agreement until registration. ASAQ (Coarsucam® and Artesunate-Amodiaquine Whintrop®, Sanofi-Aventis) was first registered in 2007, three years after technology transfer, and WHO pre-qualified in 2009. The absence of intellectual property removed barriers to access and wider production as the exclusivity was time limited and is now expired. On the other hand, this approach may be difficult to replicate in all situations; while it relies heavily on partners absorbing part of the costs and risks, or working at cost, there is clearly no or limited prospects for return for those (typically in this case, the public sector) that allocate resources and take the risks inherent in the early phases of pharmaceutical development. Together, this paper and Kauss et al provide a comprehensive account of the process that, through trials and errors, identified a suitable pharmaceutical form for industrial scale-up and was further developed for commercialization (Bompart F, Kiechel JR, Sebbag R, Pecoul B: Innovative public-private partnership to maximize the delivery of anti-malarial medicines: lessons learned from the ASAQ Winthrop experience, submitted). ASAQ is now registered and available in several malaria endemic countries.
The development of a stable co-formulation containing AS and AQ in a bilayer tablet was a result of about three years of pharmaceutical development under the public-private partnership initiated by WHO and DNDi with the not-for-profit and public sector in the lead. The development process was hampered by financial issues related to a lack of investment from the public sector. This lead initially to development goals focusing more on lowering the cost of manufacturing rather than on a more expensive optimization process based on the isolation of AS from AQ. This explains the delay in choosing an orientation towards a bilayer tablet. DNDi has since established a strong fund-raising capacity building to allow completion of the FACT and other programmes. TropiVal is now part of a scientific team aiming to develop new pharmaceutical projects on drug development for neglected diseases. Moreover, this experience demonstrates that a network of scientists associated with industrial partners is able to lead to the production of improved tools for disease control. Although fundamental research is the initial aim of a public research institution, providing experience in applied research and technology transfer is likely to improve the relationship between academia and industry.
The resulting branded product (Artesunate-Amodiaquine Whintrop®) is currently made available at manufacturing cost to the national malaria control programmes of African and Asian endemic countries. As no intellectual property right was claimed by the network of scientists and industrials involved in the initial phase of the project, an exclusivity contract allowing technology transfer was signed between DNDi and Sanofi-Aventis. The contract expired at the time of registration of ASAQ and the rights of producing and commercializing the product are now open and can be transferred to any other interested commercial partner.
artemisinin-based combination therapy
- AQH AQ:
National Centre for Research and Training in Malaria
Drugs for Neglected Disease initiative
Fixed artemisinin based combination treatment
Médecins Sans Frontières
Special Programme for Research and Training in Tropical Diseases of the WHO
World Health Organization.
The authors wish to thank Drs I. Dos Santos, M. Guyot and Mrs. AM. Lagueny for their assistance in laboratory stage pharmacotechnical development, Dr. M. Maury for clinical batch conditioning, and Drs C. Rubio and J. Susperregui for their help in drug characterization and degradation products. P. Olliaro is a staff member of the WHO; the authors alone are responsible for the views expressed in this publication and they do not necessarily represent the decisions, policy or views of the WHO.
This work was supported by UNICEF/UNDP/WB/WHO Special Programme for Research and Training in Tropical Disease (WHO/TDR) TSA T26/181/46 and European Commission ICA4-CT-2002 10046. TropiVal was supported by the Conseil Régional d'Aquitaine, France and by the Incubateur Régional d'Aquitaine, France. The mechanisms of funding of the project are detailed in the paper.
- WHO: Global anti-malarial drug policy database, Africa. 2009, WHO, [http://www.who.int/malaria/am_drug_policies_by_region_afro/en/index.html]
- Kauss T, Fawaz F, Guyot M, Lagueny AM, Dos Santos I, Bonini F, Olliaro P, Millet P: Fixed Artesunate - Amodiaquine combined pre-formulation study for the treatment of malaria. Int J Pharm. 2010, 395: 198-204. 10.1016/j.ijpharm.2010.05.038.View ArticlePubMed
- Millet P: [TropiVal: Lessons learned from attempts to valorize public research and development of new therapies for control of tropical disease]. Med Trop (Mars). 2004, 64: 441-443.
- Croft SL: Public-private partnership: from there to here. Trans R Soc Trop Med Hyg. 2005, 99 (Suppl 1): S9-14.View ArticlePubMed
- Sirima SB, Gansane A: Artesunate-amodiaquine for the treatment of uncomplicated malaria. Expert Opin Investig Drugs. 2007, 16: 1079-1085. 10.1517/135437188.8.131.529.View ArticlePubMed
- Grimm W: Extension of the International Conference on Harmonization Tripartite Guideline for Stability Testing of New Drug Substances and Products to countries of climatic zones III and IV. Drug Dev Ind Pharm. 1998, 24: 313-325. 10.3109/03639049809085626.View ArticlePubMed
- Taylor WR, Terlouw DJ, Olliaro PL, White NJ, Brasseur P, ter Kuile FO: Use of weight-for-age-data to optimize tablet strength and dosing regimens for a new fixed-dose artesunate-amodiaquine combination for treating falciparum malaria. Bull World Health Organ. 2006, 84: 956-964. 10.2471/BLT.06.031492.PubMed CentralView ArticlePubMed
- Sirima SB, Tiono AB, Gansane A, Diarra A, Ouedraogo A, Konate AT, Kiechel JR, Morgan CC, Olliaro PL, Taylor WR: The efficacy and safety of a new fixed-dose combination of amodiaquine and artesunate in young African children with acute uncomplicated Plasmodium falciparum. Malar J. 2009, 8: 48-10.1186/1475-2875-8-48.PubMed CentralView ArticlePubMed
- Haynes RK: From artemisinin to new artemisinin antimalarials: biosynthesis, extraction, old and new derivatives, stereochemistry and medicinal chemistry requirements. Curr Top Med Chem. 2006, 6: 509-537. 10.2174/156802606776743129.View ArticlePubMed
- Haynes RK, Chan HW, Lung CM, Ng NC, Wong HN, Shek LY, Williams ID, Cartwright A, Gomes MF: Artesunate and dihydroartemisinin (DHA): unusual decomposition products formed under mild conditions and comments on the fitness of DHA as an antimalarial drug. ChemMedChem. 2007, 2: 1448-1463. 10.1002/cmdc.200700064.View ArticlePubMed
- Narayanasamy J, Kubicki JD: Mechanism of hydroxyl radical generation from a silica surface: molecular orbital calculations. J Phys Chem B. 2005, 109: 21796-21807. 10.1021/jp0543025.View ArticlePubMed
- Navaratnam V, Ramanathan S, Wahab MS, Siew Hua G, Mansor SM, Kiechel JR, Vaillant M, Taylor WR, Olliaro P: Tolerability and pharmacokinetics of non-fixed and fixed combinations of artesunate and amodiaquine in Malaysian healthy normal volunteers. Eur J Clin Pharmacol. 2009, 65: 809-821. 10.1007/s00228-009-0656-1.PubMed CentralView ArticlePubMed
- Davidson C: New type of R and D cooperation spawns malaria drugs. Bull World Health Organ. 2006, 84: 6-8.PubMed CentralPubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.