Association of ADAMTS13 polymorphism with cerebral malaria
© Kraisin et al; licensee BioMed Central Ltd. 2011
Received: 24 August 2011
Accepted: 14 December 2011
Published: 14 December 2011
Cerebral malaria is one of the most severe manifestations of Plasmodium falciparum malaria. The sequestration of parasitized red blood cells (PRBCs) to brain microvascular endothelium has been shown to contribute to the pathophysiology of cerebral malaria. Recent studies reported increased levels of von Willebrand factor (VWF) and reduced activity of VWF-cleaving protease, ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13), in patients with cerebral malaria.
Association of six single nucleotide polymorphisms (SNPs) of the ADAMTS13 gene with cerebral malaria was examined in 708 Thai patients with P. falciparum malaria.
Among six SNPs, the derived allele of a SNP located in intron 28, rs4962153-A, was significantly associated with protection against cerebral malaria when 115 cerebral malaria patients were compared with 367 mild malaria patients (Fisher's exact P-value = 0.0057; OR = 0.27; 95% CI = 0.096-0.76). Significant association was also detected between 115 cerebral malaria and 593 non-cerebral malaria (226 non-cerebral severe malaria and 367 mild malaria) patients (Fisher's exact P-value = 0.012; OR = 0.30; 95% CI = 0.11-0.83).
Excessive adhesion of PRBCs to the platelet-decorated ultra-large VWF (ULVWF) appears to enhance the sequestration of PRBCs to cerebral microvascular endothelium. The genetic association observed in the present study implies that the regulation of platelet-decorated ULVWF strings by ADAMTS13 may play a role in the development of cerebral malaria.
Malaria is a major cause of morbidity and mortality in tropical countries. Approximately 250 million clinical cases of malaria occur, resulting in almost 1 million deaths each year. Cerebral malaria, one of the most serious complications of Plasmodium falciparum malaria infection, is an important cause of death. The excessive sequestration of parasitized red blood cells (PRBCs) to brain microvascular endothelium has been suggested to specifically contribute to the pathophysiology of cerebral malaria , yet the pathogenesis of cerebral malaria remains poorly understood.
It has been demonstrated that levels of von Willebrand factor (VWF) are significantly increased in malaria patients compared with non-malarial patients with fever or control subjects . Further studies confirmed release of VWF after endothelial cell activation in patients with falciparum malaria [3–5] and detected adhesion of PRBCs to platelet-decorated ultra-large VWF (ULVWF) strings . The platelet-decorated strings are cleaved and regulated by plasma protease ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13) [6, 7]. Together with the presence of abnormal circulating ULVWF multimers, a significant reduction in plasma ADAMTS13 function was also observed in cerebral or severe malaria patients compared to healthy controls . These observations suggest that decreased ADAMTS13 activity plays a crucial role in the development of cerebral malaria.
The human ADAMTS13 gene (OMIM *604134), spanning approximately 45 kb, is located on chromosome 9q34 [6, 7]. To date, a number of ADAMTS13 mutations causing congenital thrombotic thrombocytopaenic purpura (TTP), a rare life-threatening disease characterized by thrombocytopenia, microangiopathic haemolytic anaemia, renal failure, and neurological deficits including coma, have been identified [6, 8]. These symptoms are also key features of severe falciparum malaria. Although ADAMTS13 is a candidate gene for cerebral malaria, no genetic association study of ADAMTS13 in malaria patients has been conducted. This study reports a significant association of common ADAMTS13 polymorphism with protection against cerebral malaria in Thai patients with falciparum malaria.
A total of 708 Thai patients infected with P. falciparum living in north-west of Thailand, near the border with Myanmar, were recruited in this study. Malarial infection by P. falciparum was confirmed by a positive blood smear for the presence of asexual form of P. falciparum. Clinical manifestations of malaria were classified according to the definitions and associated criteria by the World Health Organization. The mild malaria group consisted of 367 patients with mild symptoms characterized by fever without other causes of infection and by lack of the manifestations of severe malaria as described below. The non-cerebral severe malaria group comprised 226 patients with severe malaria which was defined as one with one of the following signs of severity or evidence of vital organ dysfunction: high parasitaemia (> 100,000 parasites/μL), hypoglycaemia (glucose level < 22 nmol/L), severe anaemia (haematocrit < 20% or haemoglobin level < 7 g/dL), a serum creatinine level > 3.0 mg/dL. The cerebral malaria group consisted of 115 patients characterized by an unrousable coma and exclusion of other causes of coma. The patients were ≥13 years old, and the average ages of the patients with mild malaria, non-cerebral severe malaria and cerebral malaria were 25.2, 20.5 and 28.6 years, respectively. All patients underwent treatment at the Hospital for Tropical Disease, Faculty of Tropical Medicine, Mahidol University (Bangkok, Thailand). This study was approved by the institutional review board of Faculty of Tropical Medicine, Mahidol University, and the Research Ethics Committee of the Graduate School of Comprehensive Human Sciences, University of Tsukuba. Written informed consent was obtained from every patient.
Genomic DNA was extracted from peripheral blood leukocytes using a QIAamp blood kit according to the manufacturer's instruction (QIAGEN, Hilden, Germany).
SNPs analysed in this study
HGVS namea, b
Ancestral allele/derived allelea (Allele frequencyc)
T (0.854)/C (0.146)
T (0.803)/C (0.197)
C (0.213)/G (0.787)
T (0.247)/C (0.753)
G (0.921)/A (0.079)
C (0.750d)/A (0.250)
The TaqMan SNP Genotyping Assay was used to genotype six ADAMTS13 SNPs (Table 1) for 708 Thai malaria patients using 7300 Real-Time PCR System (Applied Biosystems). In this study, rs1055432 was genotyped for the Asian HapMap samples (HapMap-JPT + CHB) to investigate the LD structure of ADAMTS13. A nonsynonymous SNP (rs11575933, P475S) located in exon12 of ADAMTS13 was analysed in 128 Thai malaria patients (64 from mild malaria group and 64 from cerebral malaria group) by using the polymerase chain reaction (PCR)-direct sequencing. The PCR was performed using the following primers: 5'-ACCCAGCTGGAGTTCATGTC-3' and 5'-CCTATGACTCTGCCCTGTCC-3', and the PCR products were subjected to direct sequencing with ABI prism 3100 Genetic Analyzer (Applied Biosystems).
Deviation from Hardy-Weinberg equilibrium was tested in each malaria group by chi-square test. The allele frequency was compared between two malaria groups by Fisher's exact test. The odds ratio (OR) and 95% confidence interval (CI) were calculated by Woolf's method. In this study the direction of association was determined based on the derived allele regardless of the allele frequency. P-value ≤ 0.05 was considered statistically significant. To address the problem of multiple testings, permutation P-values were calculated for single SNPs from 100,000 permutations using the Haploview software, version 4.2. Haplotype frequency and pairwise LD parameter, r2, were estimated using the Haploview software. The haplotype association test based on the permutation procedure was conducted using the Haploview software. In the haplotype association test, to find a haplotype showing stronger association with cerebral malaria than SNP, permutation P-values were calculated for single SNPs and haplotypes from 100,000 permutations.
The association of rs4962153 with platelet count obtained from 449 malaria patients before treatment (on Day 0) was assessed by analysis of covariance (ANCOVA) adjusted for age (years), gender (female or male), and symptom (mild, non-cerebral severe, or cerebral), where dummy variables were used for symptom.
Allele frequencies of ADAMTS13 SNPs in Thai malaria patients
M (2n = 734)
S (2n = 452)
C (2n = 230)
M vs C
S vs C
M + S vs Ca
The SNP rs11575933 (P475S) has been reported to influence the proteolytic activity of ADAMTS13 [11–13]. In 128 Thai malaria patients, rs11575933-T (475S) allele was not observed. Thus, the remaining samples were not analysed, and rs11575933 was not considered in the association analysis.
LD structure and haplotype frequency
Haplotype analysis indicated that rs4962153-A was on H7 and H8 haplotypes (Figure 2b). The estimated frequencies of these haplotypes in mild malaria patients were higher than those in cerebral malaria patients, suggesting that the significant association of rs4962153-A with protection against cerebral malaria was not caused by either one of the haplotypes bearing rs4962153-A.
Platelet counts and rs4962153
The reduced activity of ADAMTS13 in malaria patients may allow overproduction of ULVWF multimers. The following adhesion of PBRCs to platelet-decorated ULVWF strings would result in excessive sequestration of PBRCs to brain microvascular endothelium. This has been proposed as one of important mechanisms of adhesion of PBRCs to endothelium in cerebral malaria . The present study revealed that rs4962153-A of ADAMTS13 was significantly associated with protection against cerebral malaria in 708 Thai malaria patients. The genetic association observed in the present study supports the above-mentioned role of ADAMTS13 in the development of cerebral malaria, although the functional significance of rs4962153-A remains to be studied. A better understanding of the regulation of platelet-decorated ULVWF strings by ADAMTS13 would provide new insights into the development of the treatment of cerebral malaria. For instance, to eliminate ULVWF and replace ADAMTS13, plasma exchange might potentially be considered a new treatment.
A previous study has shown that not only plasma ADAMTS13 activity but also plasma ADAMTS13 concentration were reduced in cerebral or severe malaria patients compared to healthy controls . Since the frequency of rs4962153-A was higher in mild malaria patients than in cerebral malaria patients, rs4962153-A may enhance the plasma level of ADAMTS13, although platelet counts were not affected by rs4962153-A (Figure 3). A recent study reported that rs4962153-A was significantly associated with susceptibility to ischemic stroke in Swedish . Considering that plasma levels of ADAMTS13 have been reported to be decreased in patients with myocardial infarction [16, 17], rs4962153-A may reduce the plasma ADAMTS13 concentration in patients with ischemic stroke. Since it seems unlikely that the transcription level of ADAMTS13 is capable of being increased and decreased by rs4962153-A depending on the situation, the detected association of rs4962153-A may be a false positive in either study. To examine the present results, the effect of rs4962153 on the transcription and/or plasma levels of ADAMTS13 in malaria patients and healthy controls should be investigated in the future.
Since variation screening of the ADAMTS13 gene for Thai malaria patients was not conducted, the possibility that the association between rs4962153 and cerebral malaria detected in the present study has been caused by LD from the other polymorphisms cannot be excluded. The analysis of LD based on the HapMap database revealed that, in the Asian HapMap populations (JPT + CHB), rs4962153 was in strong LD with rs739468, rs3124765, and rs3094379 with r2 of 1, 1, and 0.86, respectively. These three SNPs are on the chromosome 9 open reading frame 7 (C9orf7) gene, located adjacent to ADAMTS13. Flower protein, the ortholog of C9orf7 in Drosophila, has been suggested to function as a Ca2+ channel to control Ca2+ influx in insect salivary gland . The function of human C9orf7 gene remains to be studied. However, it appears unlikely that polymorphism of C9orf7 is primarily associated with cerebral malaria, since Ca2+ channel is not currently implicated in the pathogenesis of cerebral malaria. Since this is the first study reporting the significant association of ADAMTS13 polymorphism with cerebral malaria, further independent studies are required to examine whether the present finding can be replicated.
PRBCs adhere to the platelet-decorated ULVWF, leading to enhancement of the sequestration of PRBCs to cerebral microvascular endothelium. The platelet-decorated ULVWF strings are cleaved and regulated by ADAMTS13. It has been reported that plasma ADAMTS13 function is significantly reduced in cerebral malaria patients compared to healthy controls. In this study, a SNP of ADAMTS13, rs4962153, was found to be significantly associated with cerebral malaria in 708 Thai patients with P. falciparum malaria. The present findings support the hypothesis that the regulation of platelet-decorated ULVWF strings by ADAMTS13 may play a role in the development of cerebral malaria.
The authors sincerely thank all malaria patients who kindly participated in the present study. The authors also thank Dr. Aya Kawasaki, Ms. Ikue Ito, and Mr. Takumi Furuya for helpful advices in experimental techniques. The authors appreciate two anonymous reviewers concerning improvements to this paper. This study was partly supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by Takeda Science Foundation. The first author, Sirima Kraisin, is supported by a Japan-East Asia Network of Exchange for Students and Youths (JENESYS) Programme of 2010 academic year, coordinated by University of Tsukuba.
- van der Heyde HC, Nolan J, Combes V, Gramaglia I, Grau GE: A unified hypothesis for the genesis of cerebral malaria: sequestration, inflammation and hemostasis leading to microcirculatory dysfunction. Trends Parasitol. 2006, 22: 503-508. 10.1016/j.pt.2006.09.002.View ArticlePubMedGoogle Scholar
- Hollestelle MJ, Donkor C, Mantey EA, Chakravorty SJ, Craig A, Akoto AO, O'Donnell J, van Mourik JA, Bunn J: von Willebrand factor propeptide in malaria: evidence of acute endothelial cell activation. Br J Haematol. 2006, 133: 562-569. 10.1111/j.1365-2141.2006.06067.x.View ArticlePubMedGoogle Scholar
- Bridges DJ, Bunn J, van Mourik JA, Grau G, Preston RJ, Molyneux M, Combes V, O'Donnell JS, de Laat B, Craig A: Rapid activation of endothelial cells enables Plasmodium falciparum adhesion to platelet-decorated von Willebrand factor strings. Blood. 2010, 115: 1472-1474. 10.1182/blood-2009-07-235150.PubMed CentralView ArticlePubMedGoogle Scholar
- de Mast Q, Groot E, Lenting PJ, de Groot PG, McCall M, Sauerwein RW, Fijnheer R, van der Ven A: Thrombocytopenia and release of activated von Willebrand factor during early Plasmodium falciparum malaria. J Infect Dis. 2007, 196: 622-628. 10.1086/519844.View ArticlePubMedGoogle Scholar
- Larkin D, de Laat B, Jenkins PV, Bunn J, Craig AG, Terraube V, Preston RJ, Donkor C, Grau GE, van Mourik JA, O'Donnell JS: Severe Plasmodium falciparum malaria is associated with circulating ultra-large von Willebrand multimers and ADAMTS13 inhibition. PLoS Pathog. 2009, 5: e1000349-10.1371/journal.ppat.1000349.PubMed CentralView ArticlePubMedGoogle Scholar
- Levy GG, Nichols WC, Lian EC, Foroud T, McClintick JN, McGee BM, Yang AY, Siemieniak DR, Stark KR, Gruppo R, Sarode R, Shurin SB, Chandrasekaran V, Stabler SP, Sabio H, Bouhassira EE, Upshaw JD, Ginsburg D, Tsai HM: Mutations in a member of the ADAMTS gene family cause thrombotic thrombocytopenic purpura. Nature. 2001, 413: 488-494. 10.1038/35097008.View ArticlePubMedGoogle Scholar
- Soejima K, Mimura N, Hirashima M, Maeda H, Hamamoto T, Nakagaki T, Nozaki C: A novel human metalloprotease synthesized in the liver and secreted into the blood: possibly, the von Willebrand factor-cleaving protease?. J Biochem. 2001, 130: 475-480.View ArticlePubMedGoogle Scholar
- Lotta LA, Garagiola I, Palla R, Cairo A, Peyvandi F: ADAMTS13 mutations and polymorphisms in congenital thrombotic thrombocytopenic purpura. Hum Mutat. 2010, 31: 11-19. 10.1002/humu.21143.View ArticlePubMedGoogle Scholar
- The International HapMap Consortium: The international HapMap project. Nature. 2003, 426: 789-796. 10.1038/nature02168.View ArticleGoogle Scholar
- The International HapMap Consortium: A haplotype map of the human genome. Nature. 2005, 437: 1299-1320. 10.1038/nature04226.PubMed CentralView ArticleGoogle Scholar
- Akiyama M, Kokame K, Miyata T: ADAMTS13 P475S polymorphism causes a lowered enzymatic activity and urea lability in vitro. J Thromb Haemost. 2008, 6: 1830-1832. 10.1111/j.1538-7836.2008.03109.x.View ArticlePubMedGoogle Scholar
- Kokame K, Matsumoto M, Soejima K, Yagi H, Ishizashi H, Funato M, Tamai H, Konno M, Kamide K, Kawano Y, Miyata T, Fujimura Y: Mutations and common polymorphisms in ADAMTS13 gene responsible for von Willebrand factor-cleaving protease activity. Proc Natl Acad Sci USA. 2002, 99: 11902-11907. 10.1073/pnas.172277399.PubMed CentralView ArticlePubMedGoogle Scholar
- Plaimauer B, Fuhrmann J, Mohr G, Wernhart W, Bruno K, Ferrari S, Konetschny C, Antoine G, Rieger M, Scheiflinger F: Modulation of ADAMTS13 secretion and specific activity by a combination of common amino acid polymorphisms and a missense mutation. Blood. 2006, 107: 118-125. 10.1182/blood-2005-06-2482.View ArticlePubMedGoogle Scholar
- Lopez JA: Malignant malaria and microangiopathies: merging mechanisms. Blood. 2010, 115: 1317-1318. 10.1182/blood-2009-12-254060.View ArticlePubMedGoogle Scholar
- Hanson E, Jood K, Nilsson S, Blomstrand C, Jern C: Association between genetic variation at the ADAMTS13 locus and ischemic stroke. J Thromb Haemost. 2009, 7: 2147-2148. 10.1111/j.1538-7836.2009.03617.x.View ArticlePubMedGoogle Scholar
- Kaikita K, Soejima K, Matsukawa M, Nakagaki T, Ogawa H: Reduced von Willebrand factor-cleaving protease (ADAMTS13) activity in acute myocardial infarction. J Thromb Haemost. 2006, 4: 2490-2493. 10.1111/j.1538-7836.2006.02161.x.View ArticlePubMedGoogle Scholar
- Crawley JT, Lane DA, Woodward M, Rumley A, Lowe GD: Evidence that high von Willebrand factor and low ADAMTS-13 levels independently increase the risk of a non-fatal heart attack. J Thromb Haemost. 2008, 6: 583-588. 10.1111/j.1538-7836.2008.02902.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Yao CK, Lin YQ, Ly CV, Ohyama T, Haueter CM, Moiseenkova-Bell VY, Wensel TG, Bellen HJ: A synaptic vesicle-associated Ca2+ channel promotes endocytosis and couples exocytosis to endocytosis. Cell. 2009, 138: 947-960. 10.1016/j.cell.2009.06.033.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.