Archived Comments for:
Using CF11 cellulose columns to inexpensively and effectively remove human DNA from Plasmodium falciparum-infected whole blood samples
Christopher Plowe, University of Maryland School of Medicine
11 May 2012
Unfortunately, as this paper went to press the manufacturer of CF11 put column production on hold, and subsequently suspended making them indefinitely. While they may resume in the future, we are investigating and evaluating alternative inexpensive means of leukocyte depletion. For updates, please visit the Molecular Module page on the website of the WorldWide Antimalarial Resistance Network www.wwarn.org.
Competing interests
None declared
An alternative cellulose powder to CF11
Bruce Russell, Singapore Immunology Network, Biopolis, A*STAR
14 June 2012
Malaria groups at A*STAR and NUS (Singapore), Eijkman Institute for Molecular Biology(Indonesia) and SMRU (Thailand) have effectively substituted the use of CF11 with Sigma medium fiber Powder (cat # c6288) since September 2011. Preparation of the filter columns using the Sigma cellulose powder are as per Sriprawat et al 2009 (http://www.malariajournal.com/content/8/1/115) .
Competing interests
I am the corresponding author on Sriprawat et al 2009
Update on CF11 availability
11 May 2012
Unfortunately, as this paper went to press the manufacturer of CF11 put column production on hold, and subsequently suspended making them indefinitely. While they may resume in the future, we are investigating and evaluating alternative inexpensive means of leukocyte depletion. For updates, please visit the Molecular Module page on the website of the WorldWide Antimalarial Resistance Network www.wwarn.org.
Competing interests
None declared
An alternative cellulose powder to CF11
14 June 2012
Malaria groups at A*STAR and NUS (Singapore), Eijkman Institute for Molecular Biology(Indonesia) and SMRU (Thailand) have effectively substituted the use of CF11 with Sigma medium fiber Powder (cat # c6288) since September 2011. Preparation of the filter columns using the Sigma cellulose powder are as per Sriprawat et al 2009 (http://www.malariajournal.com/content/8/1/115) .
Competing interests
I am the corresponding author on Sriprawat et al 2009