A pilot study on quality of artesunate and amodiaquine tablets used in the fishing community of Tema, Ghana
© Affum et al.; licensee BioMed Central Ltd. 2013
Received: 13 March 2013
Accepted: 12 June 2013
Published: 28 June 2013
The ineffectiveness of artesunate and amodiaquine tablets in malaria treatment remains a health burden to WHO and governments of malaria-endemic countries, including Ghana. The proliferation of illegitimate anti-malarial drugs and its use by patients is of primary concern to international and local drug regulatory agencies because such drugs are known to contribute to the development of the malaria-resistant parasites in humans. No data exist on quality of these drugs in the fishing village communities in Ghana although the villagers are likely users of such drugs. A pilot study on the quality of anti-malarial tablets in circulation during the major fishing season at a malarious fishing village located along the coast of Tema in southern Ghana was determined.
Blisterpacks of anti-malarial tablets were randomly sampled. The International Pharmacopoeia and Global Pharma Health Fund Minilab protocols were used to assess the quality of anti-malarial tablets per blisterpacks allegedly manufactured by Guilin Pharmaceutical Co Ltd, China (GPCL) and Letap Pharmaceuticals Ltd, Ghana (LPL) and sold in chemical sales outlets at Kpone-on–Sea. Ferric chloride and cobaltous thiocyanate tests confirmed the presence of active ingredients in the tablets. A confirmatory test for the active ingredient was achieved with artesunate (ICRS1409) and amodiaquine (ICRS0209) reference standards. A high performance liquid chromatography analysis confirmed the amount of artesunate found in tablets.
Based on the International Pharmacopoeia acceptable range of 96/98 to 102% for genuine artesunate per tablet, 10% [relative standard deviation (RSD): 3.2%] of field-selected artesunate blisterpack per tablets manufactured by GPCL, and 50% (RSD: 5.1%) of a similar package per tablet by LPL, passed the titrimetric test. However, 100% (RSD: 2.2%) of amodiaquine blisterpack per tablet by GPCL were found to be within the International Pharmacopeia acceptable range of 90 to 110% for genuine amodiaquine in tablet, whilst 17% of a similar package per tablet by LPL failed spectrophotometric testing.
Inadequate amounts of artesunate and amodiaquine detected in the tablets suggest that both pharmaceutical companies may not be following recommended drug formulation procedures, or the active pharmaceutical ingredients might have been degraded by improper storage conditions. Thus, drugs being sold at Kpone-on-Sea, Ghana may likely be classified as substandard drugs and not suitable for malaria treatment.
KeywordsArtesunate and amodiaquine tablets Counterfeit/substandard/falsified Ferric chloride test Cobaltous thiocyanate test Titrimetric Spectrophotometry High performance liquid chromatography
Counterfeit or substandard anti-malarial drug proliferation on the Ghanaian market is a public health concern to the individual, the community and government. Malaria is endemic in sub-Saharan Africa, which includes Ghana. The World Health Organization (WHO) reports that in 2010 malaria caused an estimated 655,000 deaths worldwide, with most of the cases occurring among African children. The report predicts that over 250 million new cases of malaria occur each year and about half the world’s population is at risk of malaria. This public health hazard has attracted attention from WHO and country-specific drug and healthcare regulatory agencies that seek to reverse the trend of anti-malarial drug counterfeiting. WHO defines counterfeit drugs as: “one which is deliberately and fraudulently mislabelled with respect to identity and/or source. Counterfeiting applies to both branded and generic products; counterfeit products may include products with the correct ingredients or with the wrong ingredients, without active ingredients, with insufficient active ingredient or with fake packaging”[3, 4]. Substandard drugs are genuine but may not meet drug quality specifications claimed by the manufacturer and those set by WHO. Further, substandard drugs occasionally contain sub-therapeutic amounts of active pharmaceutical ingredients (API) and/or may show suboptimal release of API (dissolution). Thus, exposing parasites to sub-lethal concentrations of API(s). Nevertheless, the percent API in genuine medicines may also be reduced if they are degraded by extremes of temperature and humidity.
The use of substandard anti-malarial drugs is known to contribute to the increasing population of drug-resistant Plasmodium falciparum, because inaccurate dose of the active pharmaceutical ingredient (API) in the tablets are not sufficient to effectively clear the parasite from the infected individual’s body system. In Ghana, the use of chloroquine for malaria treatment has been banned because chloroquine is not effective in clearing P. falciparum in an infected person. For this reason, most malaria-endemic countries have adopted artemisinin-based combination therapy [artesunate (AS)–amodiaquine, AS–mefloquine, AS–sulphadoxine/pyrimethamine)] as first-line treatment of P. falciparum infections.
The global anti-malarial drug policy for Ghana requires that uncomplicated P. falciparum malaria infections are treated with a combined therapy of artesunate-amodiaquine and artemether-lumefantrine[6, 7]. However, in cases of failed treatment in pregnancy and severe malaria, a single dose quinine therapy has been recommended. Further, for intermittent preventive treatment a combined therapy of sulphadoxine-pyrimethamine must be used. This has made artesunate-amodiaquine tablets a target of the very sophisticated and highly profiteering substandard or counterfeit drug trade. The extremely complex labelling by pharmaceutical companies makes it more difficult for counterfeit anti-malarial tablets to be visually identified. In a survey on proliferation of anti-malarial counterfeiting between 1999 and 2002, it was found that 38 to 53% of artesunate anti-malarial tablets produced by Indian and Chinese pharmaceutical companies were counterfeited. Globally, it is estimated that more than 10% of drugs that are traded are counterfeits[10, 11]. It is possible that most of these counterfeit anti-malarial tablets are sent to underdeveloped and malaria-endemic countries, including Ghana. Although no data have been published on the level of anti-malarial counterfeiting or substandard drugs by locally (Ghanaian) based pharmaceutical companies, their contribution to the counterfeit drug trade cannot be ignored. In a study to compare quality of drug products approved by the Stringent Regulatory Authority (SRA) and WHO, it was determined that African countries had a greater proportion of SRA or WHO approved products (31.5%) than Indian cities, but experienced higher failure rates (14.21%) than Indian cities (7.83%). Further, products made in Africa had the highest failure rate of 25.77%, followed by Chinese products at 15.74%, Indian products at 3.70%, and European/US products at 1.70%; and 17.65% for Chinese products approved by the WHO. The United Nations Office on Drugs and Crime has implicated China and India as major exporters of counterfeit drugs[13–15]. Therefore, monitoring of artesunate and amodiaquine, combined or single therapy drugs, for substandard, counterfeiting and degradation is necessary to alert and prevent the spread of drug-resistant P. falciparum.
The objective of this pilot study was to evaluate the quality of artesunate and amodiaquine tablets in circulation during the major fishing season at Kpone-on-Sea, a fishing village located along the coast in southern Ghana, and to assess the extent of substandard, counterfeit, or degraded anti-malarial drugs in this unique geographical location. This research is expected to encourage WHO, health organizations and governments to be effective in their drug monitoring programmes in poor communities since they are far more likely to be the victims of poor anti-malarial drugs.
Chemicals and reagents
All chemicals were of analytical and American Chemical Standard (ACS) grade. Hydroxylamine hydrochloride, sodium hydroxide, iron (III) chloride, phosphoric acid, hydrochloric acid, phenolphthalein and bromophenol blue, ethanol, methylene chloride, chloroform, acetone and HPLC grade methanol, toluene, acetic acid, and acetonitrile were purchased from Sigma Aldrich, St Louis, MO, USA. Artesunate reference standard (ICRS1409) and amodiaquine reference standards (ICRS0209) were purchased from the European Directorate for Quality of Medicines and Healthcare (EMDQ), Strasbourg, France. The anti-malarial drugs were purchased from all available chemical stores at Kpone-on-Sea, Tema.
UV-Vis Spectrophotometer was from Shimadzu, Japan. Microcentrifuge was from France. The high performance liquid chromatography (HPLC) system consisted of a Waters 1525 Binary Pump, Waters 2487 Dual Wavelength Absorbance Detector with an online Waters degasser and inline filter. A Waters C-18 reversed phase HPLC column of dimension 3.9 mm × 150 mm and 5 μm particle size was used for the separation. The manual injection rheodyne valve has a volume of 20 μL. The chromatograms were acquired, integrated and quantified using Waters Breeze Software version 3.3. Class-A quality glassware was used in all laboratory work.
Kpone-on-Sea is one of the largest fishing village located in the Tema Municipal Health Directorate, within the Greater Accra Region of Ghana. It is located at 5°69’N, 0°06’E within the coastal savannah belt of West Africa and has a population of about 15,000 people. It is bordered on the east by Prampram, on the west by Tema, south by the Gulf of Guinea (Atlantic Ocean), and north by shrubland, beyond which is the Ghana Industrial Free Zone. It is at an altitude 50-100 m above sea level and has an equatorial climate. Temperatures range from 24.4 to 27.8°C with a mean of 26.1°C. Mean annual rainfall is between 1,133 and 3,606 mm with an average relative humidity index ranging from 78 to 85%. The land formation and the drainage patterns of the four sectors of the village are such that all water from the village drains into a stream that lies on the outskirts of the village. There is also a lagoon on the outskirts of the village. A recent study in the village showed a prevalence of malaria (11%) with a peak parasite rate of 21% in children aged one to five years. Plasmodium falciparum was the major parasite detected in all positive blood slide examinations. Most of the houses are constructed of cement and corrugated iron roofing. The majority of the residents (80%) are of the Ga and Ga-Adangbe ethnic groups. Most of the inhabitants are fishermen and a sizeable proportion is involved in vegetable farming (Figure 1). The rationale for selecting this study site was based on the level of education, population size, fishing activities, and reported incident rate of malaria infections amongst the population.
Experimental design and sampling
Commercial artesunate-amodiaquine blisterpacks were purchased randomly without alerting salepersons at public chemical sales outlets of the research objective. Random drug sampling design was done by following Global Pharma Health Fund Minilab protocol, The International Pharmacopoeia and WHO recommended format for drug sampling. The sampling unit for this study was the anti-malarial tablets that are sold from the various chemical outlets at Kpone-on Sea. A total of 32 blisterpacks containing both AS+AM tablets with the same lot or batch numbers manufactured by Guilin Pharmaceutical Co Ltd, China (GPCL) and Letap Pharmaceuticals Ltd, Ghana (LPL) were purchased between June and September in 2011 from 16 chemical sales outlets (two blisterpacks/chemical sale outlet). The lot or batch numbers were LQ110403A and 1370031 for GPCL and LPL, respectively. Each blisterpack by GPCL contained 12 each of artesunate and amodiaquine tablets whilst those by LPL contain six each of artesunate and amodiaquine tablets. The expiry date, manufacture date, source of drug, batch (lot) number, dose amount and the name of the chemical store sales outlet were recorded. All samples were stored at room temperature until analysis. Purchased blisterpacks manufactured by LPL were coded as “L#” whilst those by GPCL as “G#” (where # equals sample number). Each artesunate tablet manufactured by GPCL has a dose of 150 mg and those by LPL a dose of 306 mg. Similarly, each amodiaquine tablet by GPCL has a dose of 50 mg and those by LPL 100 mg. All drug samples were analysed before expiry date. Two blisterpacks of same batch number were purchased from each chemical sales outlet.
A sample was defined as one dosage unit: such as a blister or blisters in one package. Artesunate tablets were separated from amodiaquine tablets from each blisterpack and kept in a sealable rubber sachet. The total artesunate tablets per blister (equivalent to full treatment dose) were grounded in porcelain with a pestle into fine powder. Similarly, the total amodiaquine tablet per each blister was grounded into fine powder. Powdered tablets were kept in dry, sealable, rubber sachets at room temperature until analysed.
A qualitative test based on physical and visual colour formation was done by following WHO recommended protocol for artesunate and amodiaquine.
Packages were visually screened and compared to genuine samples when available to identify counterfeit drugs. Anti-malarial packages with following defects were classified as counterfeit: (1) apparent trademark violations or other obvious labelling infractions; (2) obvious poor packaging of product which suggested degraded product; (3) incorrect spelling of product name; (4) manufacturing date after the expiry date of product; (5) odd font or font size differed from innovator brands or branded generic originals; and, (6) fake holograms.
Ferric chloride test for artesunate
This method was adapted from the methods in The International Pharmacopoeia monograph for anti-malarials. Briefly, hydroxylamine hydrochloride solution was prepared from 3.5 g of hydroxylamine hydrochloride, 95 mL of 535 g/L ethanol, 0.5 mL of 1g/L bromophenol and sufficient 0.5 M potassium hydroxide/ethanol added until a greenish tint developed. The final solution was diluted to 100 mL with 535 g/L ethanol. Approximately 0.271 g tab/50 mg artesunate (AR) by GPCL and 0.178 g tab/ 50 mg artesunate (AR) by LPL was weighed into individual 50 mL falcon tubes and 20 mL of 95% ethanol was added. The mixture was shaken for 5 min, filtered and centrifuged at × 300 g for another 5 min. Approximately 10 mL of filtrate was transferred into a clean test tube. Approximately 0.25 mL of hydroxylamine hydrochloride solution was added, followed by 0.25 mL of 80 g/L sodium hydroxide. The test samples, negative and positive controls (prepared from ICRS1409) were boiled on a water bath. The test tubes were allowed to cool to ambient temperature after which two drops of 70 g/L hydrochloric acid was added. Finally, two drops of ferric chloride was added. A red-violet complex colour indicated a positive test and was confirmed from reference standard and negative control.
Cobaltous thiocyanate test for amodiaquine hydrochloride
This method was adapted from the protocol in the International Pharmacopoeia monograph for anti-malarials. Briefly, cobaltous thiocyanate reagent was prepared from 3.4 g cobalt chloride and 2.15 g of ammonium thiocyanate with double-distilled water in 50 mL volumetric flask. About 0.273 g of artesunate powder corresponding to each tablet dose was extracted with 0.1 M hydrochloric acid and centrifuged. Approximately 2 mL of supernatant was aliquoted into new tubes and a drop of cobaltous thiocyanate reagent added to form a green precipitate. The colour formed by amodiaquine reference standard (prepared from ICRS0209) and negative control was used to identify positive and negative reactions for the test samples.
The quantitative analysis of artesunate and amodiaquine was achieved by using the individual methods in The International Pharmacopeia monographs with little modification. The methods included titrimetic, chromatography and spectrophotometry[38, 40].
A titrimetric method was used to determine artesunate in tablets. Briefly, an artesunate powder equivalent to 0.25 mg artesunate API was weighed into 50 mL conical flask and 25 mL of neutralized ethanol (prepared from 750 g/L ethanol, 0.5 mL phenolphthalein/ethanol and sufficient carbonate-free 0.02 mol/L sodium hydroxide to produce a faint pick colour) was added and titrated against 0.05 mol/L sodium hydroxide with two drops of 0.01g/mL phenolphthalein indicator to a permanent pick colour. Each mL of sodium hydroxide consumed was equivalent to 19.22 mg anhydrous artesunate (C19H28O8). Each sample was analysed in duplicate. Positive control (prepared from ICRS1409) and negative controls were titrated as the samples.
High performance liquid chromatography (HPLC)
Artesunate in the powdered anti-malarial tablets manufactured by GPCL and LPL were determined by following a validated WHO HPLC protocol specified in the International Pharmacopoeia monograph. Briefly, the HPLC analysis was done by using Waters HPLC system set-up. A Waters HPLC C-18 column of dimensions 0.39 mm × 150 mm id and 5 μm particle size was equilibrated with a mobile phase prepared from acetonitrile and phosphate buffer at pH 3 (44:56% v/v). The absorbance wavelength for detection of artesunate was set at 216 nm. Approximately 29.95 mg of pure artesunate standard, 35.6 mg of (equivalent to 10 mg of artesunate-active pharmaceutical ingredient) artesunate tablet powder by LPL and 0.27.1 mg (equivalent to 20.1 mg of artesunate) of artesunate tablet powder by GPCL were each dissolved in 10 mL of the mobile phase, centrifuged and supernatant aliquoted into clean borosilicate glass vials. Approximately 20 μL of the artesunate standard solution, filtered tablet solution were separately analysed on the HPLC instrument using an isocratic elution flow rate of 1.0 mL/min over a 10-min run time. The retention time of artesunate reference standard was used to determine the retention time of artesunate in the powdered tablets. The concentration of artesunate in each powdered tablet was calculated from a calibration graph of peak height versus concentration of artesunate reference standard. The reproducibility of the retention time was determined.
Where 355.9 = molecular weight of amodiaquine anhydrous; 428.8 = molecular weight of anhydrous amodiaquine hydrochloride; C = concentration in μg/mL calculated with reference to the anhydrous substance of amodiaquine hydrochloride in the reference; Au = absorbance of the solution of the substance being examined; As = absorbance of reference solution.
Results were analysed by a one-sample t-test at confidence level of 95% and test value of 0.05, using Microsoft (Richmond, VA, USA) Statistical Package for Social Sciences (SPSS) version 16.0.
Qualitative analysis of anti-malarial tablets manufactured by pharmaceutical companies
All the artesunate and amodiaquine tablets manufactured by GPCL and LPL were found to contain some amount of their respective API. The positive tests for the amodiaquine and artesunate tablets were confirmed with reference standards ICRS0209 and ICRS1409 respectively. The negative control test (without the active ingredients) did not form expected complex when compared to the positive test.
Quantitative analysis of anti-malarial tablets manufactured by pharmaceutical companies
Percentage of artesunate and amodiaquine anti-malarial tablet per blisterpack manufactured by GPCL and LPL determined by methods in International Pharmacopoeia monograph
Anti-malarial blisterpack identification number
Percentage of artesunate per tablet (%)
Classification based on PhInt acceptable range of 96/98 – 102%
Percentage of amodiaquine per tablet (%)
Classification based on PhInt acceptable range of 90 – 110%
Weight of each anti-malaria tablet/mg dose manufactured by Pharmaceutical companies
Name of anti-malaria tablet
Weight of tablet/ API dose (g/mg)
Number of tablet/blisterpack
Weight of total tablet in blisterpack per dose API (g/mg)
Guilin Pharmaceutical Co., Ltd
3.27 ± 0.03/600
(0.01, 0.8 %)†
Letap Pharmaceutical Company
2.13 ± 0.02/600
(0.2, 0.9 %)†
Guilin Pharmaceutical Co., Ltd
3.26 ± 0.01/1800
(0.04, 0.4 %)†
Letap Pharmaceutical Company
The results obtained for HPLC analysis of artesunate powder confirmed the amount of active pharmaceutical ingredient obtained from the titrimetric analysis was calculated from y = 2.0E+06x. R2 = 1.0. A retention time of 6 min was obtained for artesunate over a total run time of 10 min. The RSD between blisterpack of anti-malarial drugs manufactured by GPCL and LPL was 2.1% and 5.1%, respectively. The high RSD for tablets manufactured by LPL could most likely be due to poor quality assurance and quality control during the drug formulation process, confirmed by the one sample student t-test analysis of the results obtained in this study. An UV/Vis spectrophotometric method was not used to determine the amount of artesunate but amodiaquine in the powdered tablet because artesunate has low molar extinction co-efficient and its UV/Vis spectra or fluorescence property is not distinct.
The amount of amodiaquine-active ingredient detected by the spectrophotometric method was not confirmed by HPLC because of the complexities often associated with the use of ion-pairs. Interestingly, the gold standard method recommended by WHO for amodiaquine is the spectrophotometric method.
The very low amodiaquine API found in amodiaquine blisterpack could be caused by poor formulation practice, poor storage conditions and packing of tablets in the blisterpack. Overall, these anti-malarial drugs with subtherapeutic amounts of active ingredients could likely lead to treatment failure, drug-resistant parasite, health hazards, spread of anti-malarial-resistant P. falciparum, and, finally death of the malaria-infected individuals.
Quantitative analysis of tablets manufactured by GPLC, China and LPL, Ghana, which was reviewed under the International Pharmacopoeia standards for anti-malarial drugs, found variable amounts of artesunate-active ingredients in their tablets. However, amodiaquine tablets from both companies seem to indicate the expected amounts of amodiaquine-active ingredients except one blisterpack by LPL, which had exceptionally low amounts of the active ingredient. The variable amount of artesunate-active ingredient in the tablets may be due to inadequate storage conditions in chemical shops or less active ingredient added during the drug formulation process, which can confound drug quality assessment and detection of substandard drug samples. Therefore, it is recommended that the assessment on quality of artesunate and amodiaquine tablets be tested at first point-of-entry of the drugs into Ghanaian and other markets. Second, independent routine testing of these drugs be done at the dispensers, hospitals or pharmacies and results reconciled to first point-of-entry before the drugs are passed to the patient. In order to combat substandards, drug receiving points along the supply chain, such as customs border-points or at specific geographic locations where a high estimated percentage of drugs is passed, be equipped with the appropriate analytical tools to detect counterfeit or substandard drugs before they are inventoried and distributed to consumers. Although some organic impurities and degradants in amodiaquine and artesunate tablets have been found during other research work, future lines of research will look for new impurities that might be harmful to patient. The presence of unknown active ingredients was not detected in both artesunate and amodiaquine tablets although such phenomenon has been detected in some anti-malarial tablets, but before the 1999 WHO data on anti-malarial counterfeiting. Thus, this study confirms the predominant existence of substandard artesunate and amodiaquine tablets in circulation at Kpone-on-Sea in Tema. Finally, the presence of substandard artesunate and amodiaquine tablets at Kpone-on-Sea must alert health care and drug regulatory agencies to be vigilant and continually to monitor these drugs to save preventable patient mortalities. In order to prevent proliferation of poor quality artesunate and amodiaquine tablets from jeopardizing the unprecedented progress and investment made in the control and elimination of malaria infections, the police, scientists, the pharmaceutical industry, governments and WHO must work together to combat the problem. Pharmaceutical companies must be alerted about these substandard artesunate and amodiaquine so that better drug formulation procedures will be followed, as well as improvement made in the storage conditions for these drugs. Finally, the recent spread of artemisinin resistance from East Asia to African countries, present a new challenge and a danger to human health which needs to be solved.
We express our appreciation to the Director, Management and entire Staff of Kasapreko Company Limited, Tema. In addition, we thank Ghana Atomic Energy Commission, Accra and Cocoa Research Institute of Ghana, Akim-Tafo for the laboratory space and transport for this research study.
This study was partially supported by Kasapreko Company Limited, Tema, Ghana [AOA2011]. The study sponsors had no role in the study design, the collection, analysis, or interpretation of the data, the writing of the report, or the decision to submit the paper for publication.
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