Cellular and humoral immune responses against the Plasmodium vivax MSP-119 malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil
© Riccio et al.; licensee BioMed Central Ltd. 2013
Received: 23 July 2013
Accepted: 10 September 2013
Published: 16 September 2013
Plasmodium vivax merozoite surface protein-1 (MSP-1) is an antigen considered to be one of the leading malaria vaccine candidates. PvMSP-1 is highly immunogenic and evidences suggest that it is target for protective immunity against asexual blood stages of malaria parasites. Thus, this study aims to evaluate the acquired cellular and antibody immune responses against PvMSP-1 in individuals naturally exposed to malaria infections in a malaria-endemic area in the north-eastern Amazon region of Brazil.
The study was carried out in Paragominas, Pará State, in the Brazilian Amazon. Blood samples were collected from 35 individuals with uncomplicated malaria. Peripheral blood mononuclear cells were isolated and the cellular proliferation and activation was analysed in presence of 19 kDa fragment of MSP-1 (PvMSP-119) and Plasmodium falciparum PSS1 crude antigen. Antibodies IgE, IgM, IgG and IgG subclass and the levels of TNF, IFN-γ and IL-10 were measured by enzyme-linked immunosorbent assay.
The prevalence of activated CD4+ was greater than CD8+ T cells, in both ex-vivo and in 96 h culture in presence of PvMSP-119 and PSS1 antigen. A low proliferative response against PvMSP-119 and PSS1 crude antigen after 96 h culture was observed. High plasmatic levels of IFN-γ and IL-10 as well as lower TNF levels were also detected in malaria patients. However, in the 96 h supernatant culture, the dynamics of cytokine responses differed from those depicted on plasma assays; in presence of PvMSP-119 stimulus, higher levels of TNF were noted in supernatant 96 h culture of malaria patient’s cells while low levels of IFN-γ and IL-10 were verified. High frequency of malaria patients presenting antibodies against PvMSP-119 was evidenced, regardless class or IgG subclass.PvMSP-119-induced antibodies were predominantly on non-cytophilic subclasses.
The results presented here shows that PvMSP-119 was able to induce a high cellular activation, leading to production of TNF and emphasizes the high immunogenicity of PvMSP-119 in naturally exposed individuals and, therefore, its potential as a malaria vaccine candidate.
KeywordsMalaria Plasmodium vivax MSP-119 Cellular response Antibodies
Malaria remains a serious public health problem causing high levels of morbidity and mortality in malaria-endemic regions. There were an estimated 219 million cases of malaria and 660,000 deaths in 2010 . Among the five Plasmodium species responsible for natural infection of human, Plasmodium vivax has the widest geographical distribution, being the second leading cause of malaria . Although usually considered a benign infection, severe P. vivax malaria cases have been reported worldwide [2–10]. In Brazil, P. vivax accounts for around 85% of clinical cases .
Since an effective malaria vaccine has long been envisaged as a potential tool for malaria control, two important points for its development are the identification of antigens that elicit the relevant immunological machinery and the correlation between the resulting immune system products and the clinical and/or parasitological protection induced. In this context, several antigens are being evaluated in clinical trials. To date, one candidate vaccine is currently being assessed in Phase 3 clinical trials and approximately 20 others in Phase 1 or Phase 2 trials . Among these antigens, P. vivax merozoite surface protein-1 (PvMSP-1) is a promising candidate.
MSP-1 is the most abundant and best-studied blood-stage antigen . MSP-1 is a 190–230 kDa protein present in almost all Plasmodium species, being synthesized in a precursor form during schizogony. Post-translational proteolytic processing of the MSP-1 precursor molecule generates different fragments (83, 28–30, 38–45 and 42 kDa). The 42 kDa fragment is processed to a 33 kDa and a 19 kDa fragments, leaving a membrane-anchored 19 kDa fragment (MSP-119) on the parasite surface after its internalization in the erythrocyte [13–15].
The potential of PvMSP-1 as a vaccine candidate is based on previous studies that reported that it is highly immunogenic under natural conditions of exposure [16–24] and that it could partially protect Saimiri monkeys . Several studies have provided evidences that MSP-119 is a target for protective immunity against asexual blood stages of malaria parasites [26–28]. This protective immunity has been shown to correlate with levels of anti- MSP-119 antibodies and it is also dependent of CD4 T cells [27, 29, 30].
Given the cumulative data supporting the potential of PvMSP-1 as a malaria vaccine, and the substantial data generated through studies in human indicating that both humoral and cellular immune responses are needed to protect against malaria, the present study aims to evaluate the acquired cellular and antibody immune responses against PvMSP-119 in individuals naturally exposed to P. vivax or Plasmodium falciparum infections in a malaria-endemic area in the north-eastern Amazon region of Brazil.
Study setting, participants, and blood collection
The study was carried out in Paragominas (47°36′ 09.63" W, 03°12′ 11.02" S), Pará State, in the Brazilian Amazon. The samples were collected in 2004. The individuals were studied by means of a questionnaire, whereby all relevant information, including personal and epidemiological data, were collected.
Written informed consent was obtained from all volunteer donors and 10 ml of venous blood samples were drawn in Vacutainer® EDTA tubes (Becton Dickinson, Oxnard, CA) from 35 individuals with uncomplicated malaria at the Hospital Municipal de Paragominas (HMP). Blood samples from 17 individuals living in Paragominas but with no history of current or previous malaria episodes were included in the study as non-infected control individuals. Blood collection was performed at the day of diagnosis and patients were treated, according to the Brazilian Ministry of Health standards for malaria therapy, immediately after blood sample collection.
Thin and thick blood smears were examined for identification of malaria parasite and determination of parasitaemia by two expert malaria microscopists from HMP and from the Laboratory of Malaria Research (Fiocruz, Rio de Janeiro, Brazil) which is a reference centre in malaria diagnosis for the Brazilian Ministry of Health. Blood smears from all subjects were stained with Giemsa and examined under 1,000-fold magnification. Parasitaemia was determined by counting parasites in reference to 200 white blood cells in thick blood films, and the number of the blood parasites per millilitre was calculated.
Nonendemic control blood samples from five individuals of the laboratory staff (Rio de Janeiro, Brazil) who had neither history of malaria nor contact with a malaria transmission area, were included in the study as 'Rio controls’. The study was reviewed and approved by the Fundação Oswaldo Cruz and Instituto Evandro Chagas Ethical Committees.
Isolation of peripheral blood mononuclear cells (PBMC)
The blood samples were centrifuged for 10 min, 400 × g and, after removal of plasma, the corresponding volume of RPMI-1640 (Sigma, St. Louis, Mo) medium containing 15 mM glutamin (Sigma), 10 mM Hepes (Sigma), 200 U/ml penicillin (Gibco), 200 μg/ml streptomycin (Gibco), 3 mg/ml gentamicyn (Sigma) and 2 g/L sodium bicarbonate (Sigma) was added. Subsequently, PBMC were isolated by density gradient centrifugation (Fycoll-Hypaque) and were washed twice in serum-free RPMI 1640 medium (Sigma). The cells were cryopreserved according to the method described by Ichino and Ishikawa . Briefly, cells were resuspended in 4°C RPMI-1640 supplemented with 40% foetal calf serum with an equal volume of cold RPMI-1640 containing 20% dimethyl sulfoxide (Sigma) and transferred to cryotubes that were immersed in a cold ethanol bath (4°C) and placed in a -70°C freezer for at least 12 h. The samples were then transferred to a liquid nitrogen storage tank. After freezing for up to 30 days, the cells were thawed and the viability was assessed using trypan blue staining. The thawing of PBMC was performed by the immersion of cryotubes in a 37°C water-bath, followed by two washes in RPMI-1640 medium (4°C) for 10 min, 400 × g.
Recovery and viability after cryopreservation
The number of viable cells was verified immediately after thawing of PBMC (ex-vivo) by cytometry flow using 7-aminoactinomycin D (7-AAD). This rapid and sensitive method allows the discrimination of live cells from apoptotic or necrotic cells . The cells were then incubated for 20 min at 4°C with 300 μl of 10 μg/ml of 7-AAD (Sigma) in phosphate-buffered saline (PBS) containing 2% foetal calf serum (Hyclone) and 0.1% sodium azide. After incubation, 300 μl of 2% paraformaldehyde were added to the samples. Labelled samples were analysed in a FACSCalibur (Becton Dickinson and Company, Franklin Lakes, USA) and red fluorescence from 7-AAD was filtered through a 675 nm long pass filter. Approximately 10,000 events were analysed for each sample.
Plasmodium falciparum crude extracts preparation
The PSS1 P. falciparum strain (Peixoto de Azevedo, Brazil) was cultivated in vitro according to the method described by Trager and Jensen . The P. falciparum crude extract was obtained from infected erythrocytes with parasitaemia above 6%. Parasitized erythrocytes with a predominance of schizonts were washed three times with PBS. The lysis of infected erythrocytes was done by addition of 0.1% saponin and gentle shaking for 15 min. The lysates were ultrasonicated in the presence of 1 mM phenylmethylsulfonyl fluoride and centrifuged 7,000 × g for 15 min at 4°C in order to eliminate the cellular debris.
Cellular proliferation assay
Cellular proliferation was analysed by using the vital staining carboxyfluorescein diacetate succinimidyl ester (CFSE, Molecular Probes). The PBMC were ressuspended in 1 ml of PBS plus 0,01% bovine serum albumin (BSA, Sigma) at 37°C. Two microlitres of a stock solution of CFSE (5 μM) were added in 1 ml of cellular suspension containing up to 1x106 cells/ml. The samples were then incubated for 10 min at 37°C. Five millilitres of RPMI-1640 at 4°C were added and the samples were incubated for 5 min in ice-bath. After incubation, the cells were washed three times with RPMI-1640 and then ressuspended with 1 ml RPMI-1640 (Sigma) medium containing 15 mM glutamin (Sigma), 10 mM Hepes (Sigma), 200 U/ml penicillin (Gibco), 200 μg/ml streptomycin (Gibco), 3 mg/ml gentamicin (Sigma), 3,7 g/l sodium bicarbonate (Isofar), 100 mM piruvate (Sigma),14 mM β-mercaptoetanol (Sigma) and 10% of inactivated foetal calf serum (FCS) (complete medium). The PBMC were distributed 2.5 × 105 cells/well in triplicate in 96-well flat-bottom microtiter plates (Falcon) in final volume of 200 μl of complete medium alone or in the presence of 5 μg/ml phytohaemagglutinin (PHA), 10 μg/ml P. falciparum crude extract or PvMSP-119 (kindly provided by Dr. Mauricio Rodrigues and Dra. Irene Soares) and, then, incubated at 37°C in 5% CO2 for 48 or 96 h. After culture, the cellular proliferation was analysed using a FACSCalibur flow cytometer.
Determination of cellular activation
The determination of cellular activation was done by using monoclonal antibodies against CD25, CD4, CD8 and CD21. The protocol consisted of the addition of 5-10 μl of the optimal antibody dilution to 5 × 105 cells in 50 μl PBS containing 2% foetal calf serum and 0.1% sodium azide (azide PBS), followed by incubation for 20–30 min at 4°C. After two washes with PBS, the cells were resuspended in 200 μl of azide PBS. The cells were then fixed with a 2% paraformaldehyde solution and maintained in the dark until the analysis in a FACSCalibur flow cytometer. At least 10,000 events were analysed.
Enzyme-linked immunosorbent assay (ELISA) for PvMSP-119
Microtiter 96-well plates (Nunc Maxisorp) were coated overnight at room temperature with 50μl of 4μg/ml PvMSP-119 protein in 0,05 M carbonate-bicarbonate buffer, pH 9.6. Plates were washed three times with PBS containing 0,05% Tween 20 (PBS/T20). Uncoated sites were blocked for 2 h at room temperature with 200μl of PBS/T20 containing 5% powdered-milk. After incubation, 50μl of the plasma sample diluted 1:50 in powdered-milk-containing PBS/T20 were added and the plates were incubated for 2 h at room temperature.
Plates were washed thrice with PBS/T20 and 50μl/well of mouse anti-human IgE, IgM, IgG or IgG subclass (Sigma) peroxidase conjugated, in optimal antibody dilution in PBS, was added. Plates were incubated for 1 h at 37°C. After washing the plates with PBS/T20, 50 μL of a solution containing 1 mg/ml of OPD (Sigma) and 15 μl of 30% H2O2 in citrate-phosphate buffer pH 5.0 were added. After incubation for 10–15 min at room temperature in the dark, the reaction was stopped with 50 μl/well of H2SO4. The absorbance was read at 405 nm in a spectrophotometer (Spectra Max 250; Molecular Devices, Sunnyvale, CA).
The cut-off value was determined as the mean optical density (OD) plus two standard deviations from controls that never visited malaria endemic areas. To standardize the OD data obtained in different experiments, OD index was calculated for each immunoglobulin determination as the ratio of the observed OD to the cut-off values. A sample with an OD index > 1.0 was considered positive.
Assays for cytokine detection
The cytokine levels in plasma and supernatant cultures samples were measured by ELISA using reagents from BD Biosciences Pharmingen, USA. Briefly, 100 μl of the capture monoclonal anti-human TNF, IFN-γ or IL-10 antibodies in optimal dilutions were used to coat 96-well plates for 14 h at 4°C. After washing and blocking, 100μl of plasma samples diluted 1:2 or supernatant of the cultures were added to duplicated wells and incubated for 24 h at 4°C. After the samples were washed, biotinylated anti-human cytokine were added and the plates were incubated for 1 h at room temperature. The presence of bound antibodies was detected using streptavidin-peroxidase (Sigma Chemical Co., St Louis, USA) for 30 min at room temperature, followed by the addition of 2,2′-azinobis (3-ethylbenzthiazolinesulfonic acid) (ABTS; Sigma Chemical Co., St Louis, USA) and 30% hydrogen peroxide (Merck, Darmstadt, Germany) as the substrate. The reaction was stopped with 20% sodium dodecyl sulphate (Merck, Darmstadt, Germany), and the absorbance was read at 405 nm in a spectrophotometer (Spectra Max 250; Molecular Devices, Sunnyvale, CA). A standard curve was constructed for each cytokine by using different dilutions of human recombinant cytokines.
For unpaired analyses, the nonparametric Mann–Whitney test was used to determine the significance of differences in plasmatic concentrations of cytokines between patients with acute malaria infection and control individuals and also among patient antibody responses. The Spearman rank correlation coefficient test was used to evaluate the correlation of epidemiological and immunological data. Student’s t-test was used to analyse differences in mean values, and chi-square analysis was applied to compare the prevalence of positive responses. Spearman rank coefficient test was also used to analyse the variable correlations. p values less than 0.05 were considered significant.
Characteristics of study groups
Blood collection was performed at the day of diagnosis, before malaria treatment. Patients sought health care at Brazilian health services 6.8 ± 6.9 days after onset of symptoms. Throughout the study period, a total of 35 samples were collected from uncomplicated malaria cases (malaria group) with P. falciparum (n = 12) or P. vivax (n = 23) infection and from individuals (n = 17) living also in downtown Paragominas but with no history of current or previous malaria episodes (control group).
The malaria group consisted of 6 female and 29 men with age ranging from 12 to 67 years old (31 ± 14 years old). Twenty six percent denied prior malaria infection and 64% reported 4.7 ± 6.0 previous malaria episodes during life. The individuals from the malaria group claimed living in malaria endemic area for 19 ± 13 years. All of them presented, at time of blood sampling, symptoms and positive thick blood smears, with a parasitaemia ranging from 50 to 3,000 (1,213 ± 1,088) parasites/μl for P. falciparum and 75 to 5,000 (1,640 ± 1,220) parasites/μl for P. vivax.
Individuals from the control group claimed living in malaria endemic area for 18 ± 13 years and corresponded to 10 women and seven men with average age of 35 ± 7 years. All individuals were negative for malaria parasites as assessed by thick blood films.
Viability of peripheral blood mononuclear cells (PBMC) after thawing
The viability of mononuclear cells after thawing was analysed and the percentages observed in malaria patients (78.1 ± 12.5%) were similar to those found in healthy individual (78.7 ± 19.6%). Likewise, no difference was observed when comparing the viability of PBMC from P. falciparum and P. vivax malaria patients.
Phenotypic analysis of ex-vivo PBMC samples
Ex-vivo analysis of CD4 + , CD8 + T cells and B cells (CD20 + ) from malaria patients and control individuals
45,6 ± 15,7
45,3 ± 12,4
23,4 ± 7,9
24 ± 10,3
20,1 ± 22,9
10,7 ± 9,6
Cellular activation ex-vivo and after 96 hours culture
The cellular activation was analysed by the expression of interleukin-2 receptor (IL-2R), using an anti-CD25 monoclonal antibody.
Plasmatic cytokine levels
In malaria patients, no correlation was observed between levels of plasmatic TNF or IFN and age, the number of previous malaria infection, time of residence in malaria endemic area, parasitaemia or the time elapsed between first symptoms and malaria diagnosis. However, a negative correlation was detected between plasmatic levels of IL-10 and the number of previous malaria infection (p = 0.0186; r = - 0.3958) in malaria individuals.
Cytokine levels from culture supernatant of PBMC
Regarding to IL-10, positive correlation was observed between the levels of this cytokine obtained from supernatants of PBMC stimulated with PvMSP1-19 (P = 0.0288, r = 0.3752) or with PSS1 (P = 0.033, r = 0.3661) and the time of residence in endemic area. Positive correlation was also observed between the number of previous malaria infection and the levels of IFN-γ when PBMC were stimulated with PvMSP-119 (P = 0.0004, r = 0.5704).
Humoral antibody response against PvMSP-119
Positive correlations were observed between anti-PvMSP-119 IgG antibodies and IL-10 levels in P. vivax- malaria patients (p = 0,0171; r = 0,7621); anti-PvMSP-119 IgM antibodies and IFN-γ levels in P. falciparum-malaria patients (p = 0,0036; r = 0,7666) and; anti-PvMSP-119 IgE antibodies and TNF in both P. vivax- and P. falciparum-malaria patients (P. vivax: p = 0,0432; r = 0,4081; P. falciparum: p = 0,0455; r = 0,5856).
In the present work, the profile of acquired cellular and antibody immune responses against PvMSP-119 was evaluated in individuals naturally exposed to P. vivax and P. falciparum infections in a malaria-endemic area in the north-western Amazon region of Brazil.
It is well known that suboptimal cryopreservation could result in a significant decrease of cell number and viability, causing alterations on the cellular phenotype and on the immune response to specific antigens. To avoid troublesome events, a protocol to successfully recovery PBMC after freezing and thawing was previously standardized . Thus, in the present study, an important frequency of mononuclear cell viability after thawing was observed (around 78%), regardless the individuals being infected or not. Because 75% PBMC viability is required for lymphocyte proliferation assays, representing the ability of cells to respond to the antigenic or mitogenic stimulation, independently of the nature or intensity of the stimulus , the cryopreservation procedure seems not have been a limiting factor in the present study.
The phenotypic analysis of ex-vivo PBMC revealed that CD4+ T cells were found more frequently than CD8+ and CD20+ cells, in both malaria and control individuals. In malaria individuals, a higher prevalence of activated CD4+ than CD8+ T cells was observed, in both ex-vivo and in 96 h culture in presence of PvMSP-119 and PSS1 antigen. It can be speculated that CD4+ T cells were the most stimulated cells due the nature of antigenic stimulus because CD4+ T cells are activated by the parasite in blood stage, while CD8+ T cells are most often activated during the hepatic stage of parasite life cycle .
Low proliferative responses in the presence of MSP-119 of P. falciparum and Plasmodium chabaudi chabaudi have already been demonstrated [37, 38]. The results also showed a low proliferative response against PSS1 crude antigen or PvMSP-119 after 96 h culture. This low proliferative response may occur because the majority of activated cells undergo activated-induced cell death, an active cell suicide mechanism of widespread biological importance that constitutes the physiological response of normal cells to activation and is believed to control the number of antigen-stimulated cells during the immune response .
Anti-inflammatory cytokines are involved in a feedback mechanism to regulate the expression of pro-inflammatory cytokines, and prevent the pathological effects that may result from their continuous secretion. Previous in vitro studies have shown that IL-10 suppresses the expression of malaria parasite-induced production of TNF by PBMC . In fact, in the present study, high plasmatic levels of IL-10 and low plasmatic levels of TNF were observed in malaria patients.
Parasite clearance seems to be related to IL-10 and IFN-γ levels. Here, an intense secretion of IFN-γ and IL-10 as detected by dosage of plasmatic levels, were shown in malaria patients. However no correlation was found between parasitaemia and IFN-γ or IL-10 levels. The high levels of IL-10 and IFN-γ are in agreement with previous clinical reports that IL-10 is up-regulated in concern with IFN-γ [41, 42], suggesting that IL-10 may be up-regulated as a direct consequence of IFN-γ production as part of homeostatic feedback mechanism to limit IFN-γ-mediated pathology, as is seen in murine malaria infections .
IL-10 levels were similar in P. vivax and P. falciparum infected individuals. However, besides plasmodial species, other factors, like the number of previous malaria infections, may influence the levels of this and other cytokines. In fact, a negative correlation between number of previous malaria infections and IL-10 levels was found. In addition, previous studies have shown that an adaptive type 1 regulatory CD4+ cells have been identified as the main source of IL-10 in experimental murine infection with Plasmodium yoelii, while in P. chabaudi infections in mice, an activated effector TH1 cells were major IL-10 producers . Understanding how these regulatory cells are induced could help to explain differences in IL-10 production in human malaria.
High production of TNF in malaria individuals is related to the development of severe malaria. In the present work, lower than expected plasmatic TNF levels (lower than those observed in control individuals) were detected in malaria patients. Interestingly, PvMSP-119-stimulated PBMCs from malaria patients showed higher TNF response than those recorded in both PSS1-stimulated and non-stimulated PBMCs. The production of TNF in recall to PvMSP-119 may suggest a protective role of PvMSP-119 immune response because TNF has been related to parasite clearance.
A great majority of studied individuals presented antibodies against PvMSP-119, independently of the reported number of previous malaria attacks or the time of residence in a malaria-endemic area, indicating that this protein is immunogenic in natural conditions of exposure and seems to be independent of the time of exposure. The high immunogenicity of PvMSP-119 may be reflex of its limited polymorphism, because independent groups demonstrated that PvMSP-119 is conserved [17, 46, 47]. Another possible explanation is that PvMSP-119 is the single fragment that remains on the parasite surface during red cell invasion through glycosylphosphatidylinositol (GPI) anchor  and studies have described GPI as a potent agonists of toll-like receptors that may provide the adjuvant required for stronger immune responses .
Anti-PvMSP-119 antibodies were not detected in the sera of five individuals from malaria group (one with vivax and four with falciparum malaria). The absence of anti-PvMSP-119 antibodies could be related to immunosuppression observed in humans in malaria infection [49, 50], however, this hypothesis seems unlikely because these non-responders individuals presented antibodies against PSS1 crude antigen. Another possible explanation for the absence of these antibodies is that PvMSP-119-specific B cells were present, although circulating antibodies titers were undetectable. Alternatively, the lack of PvMSP-119 antibody response may be due the genetic restriction of immune response that has been described to several plasmodial recombinant proteins and synthetic peptides [24, 51–55]. However, in a recent study performed in the Brazilian Amazon region, no association between HLADRB1* and HLADQB1* allelic groups and the antibody response against PvMSP-119 was found .
Considering that different Plasmodium antigens in the same population as well as the same Plasmodium antigen in different populations can induce different antibody profiles, it was also evaluated the frequency and levels of IgG, IgM and IgE class and IgG subclass against PvMSP-119. IgM antibodies were the most prevalent and the one with the highest levels. PvMSP-119-induced IgG antibodies were predominantly of non-cytophilic subclasses. Different data have been reported for individuals living in other Brazilian-endemic areas with different levels of exposure where the PvMSP-119-induced antibodies were predominantly of IgG1 subclass [20, 23]. Differences in antibody profile may be due to transmission intensity. In fact, previous study has demonstrated that the levels of IgG1 and IgG3 specific antibodies were low among individuals with long-term exposure (~19 years) when compared to subjects less and sporadically exposed (< 1 year) . In this concern, one must emphasize that the individuals from malaria group claimed living in malaria endemic area for 19 years. Another additional hypothesis could be related to cytokine modulation of specific antibodies production because IL-10 has been reported to demonstrate both potentiating and inhibiting IgE and increasing IgG4 productions  as well as to be associated with IgM antibodies against P. vivax. In addition, associations between polymorphism in cytokine genes and anti-plasmodial antibody response have been reported . One other possibility could be the context of the response because co-infection with helminths is known to shift the Th1 to the Th2 pattern of immune response modulating the IgG subclass expression. Independently of its origin, the differences between antibody profile reported in the present study and the aforementioned underline the importance of conducting immunoepidemiological studies in different malaria endemic areas where transmission intensities and human genetic background are different.
Associations between antibody responses with time of residence and/or number of previous episodes have been commonly reported to several malaria antigens [52, 58, 59]. However, in the present study, no association between levels of PvMSP-119 antibodies and number of previous malaria episodes was observed. This finding may reflect that people living for a longer period of time in the region may have acquired some degree of clinical immunity after experiencing a number of infections, therefore, reporting less episodes of clinical malaria in the more recent years. Interestingly, only the levels of IgE antibodies were inversely correlated with the number of previous malaria episodes. Thus, it can be supposed that higher levels of IgE antibodies may have an important role against clinical malaria. Similar results were already reported that shown that high levels of malaria specific IgE were associated with reduced risk for subsequent clinical malaria episodes .
In this study the frequency and the levels of IgG, IgM and IgE antibodies against PvMSP-119 were higher in individuals infected with P. vivax than P. falciparum or who had reported the last malaria episode due P. vivax. S imilar results were reported elsewhere where sera from subjects who had had P. vivax in their last malaria clinical episode presented higher levels of antibodies when compared with those whose last malaria episode was due to P. falciparum.
The individuals infected with P. falciparum studied in this work reported previous infection with P. vivax. This may explain the recognition of PvMSP119 antigen by antibodies from individuals infected with P. falciparum. However, the similarity between PfMSP1 and PvMSP1 antigens may also explain, at least in part, the recognition of PvMSP1 by patients infected with P. falciparum, although a recent study has shown that sera from immunized mice with PfMSP119 or PvMSP119 failed to cross-react with heterologous antigen .
In conclusion, the results presented here shows that PvMSP-119 was able to induce a high cellular activation leading to production of TNF, emphasizing the high immunogenicity of PvMSP-119 in naturally exposed individuals and therefore its potential as a malaria vaccine candidate.
We are grateful to all patients that agreed to participate in this study for their cooperation and generous donation of blood, which made this study possible. This work was supported by Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ, Brazil), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil), Instituto Oswaldo Cruz (FIOCRUZ, Brazil). MFFC and CTDR are recipients of a Research Productivity Fellowship from CNPq and receive a grant from FAPERJ as "Cientistas do Nosso Estado".
- WHO: World Malaria Report 2012. 2012, Geneva: World Health Organization,http://www.who.int/malaria/publications/world_malaria_report_2012/en/,
- Barcus MJ, Basri H, Picarima H, Manyakori C, Sekartuti , Elyazar I, Bangs MJ, Maguire JD, Baird JK: Demographic risk factors for severe and fatal vivax and falciparum malaria among hospital admissions in northeastern Indonesian Papua. Am J Trop Med Hyg. 2007, 77: 984-991.PubMed
- Tjitra E, Anstey NM, Sugiarto P, Warikar N, Kenangalem E, Karyana M, Lampah DA, Price RN: Multidrug-resistant Plasmodium vivax associated with severe and fatal malaria: a prospective study in Papua, Indonesia. PLoS Med. 2008, 5: e128-10.1371/journal.pmed.0050128.PubMed CentralView ArticlePubMed
- Genton B, D’Acremont V, Rare L, Baea K, Reeder JC, Alpers MP, Muller I: Plasmodium vivax and mixed infections are associated with severe malaria in children: a prospective cohort study from Papua New Guinea. PLoS Med. 2008, 5: e127-10.1371/journal.pmed.0050127.PubMed CentralView ArticlePubMed
- Kochar DK, Das A, Kochar SK, Saxena V, Sirohi P, Garg S, Kochar A, Khatri MP, Gupta V: Severe Plasmodium vivax malaria: a report on serial cases from Bikaner in northwestern India. Am J Trop Med Hyg. 2009, 80: 194-198.PubMed
- Anstey NM, Russell B, Yeo TW, Price RN: The pathophysiology of vivax malaria. Trends Parasitol. 2009, 25: 220-227. 10.1016/j.pt.2009.02.003.View ArticlePubMed
- Alexandre MA, Ferreira CO, Siqueira AM, Magalhães BL, Mourão MP, Lacerda MV, Alecrim MG: Severe Plasmodium vivax malaria, Brazilian Amazon. Emerg Infect Dis. 2010, 16: 1611-1614. 10.3201/eid1610.100685.PubMed CentralView ArticlePubMed
- Manning L, Laman M, Law I, Bona C, Aipit S, Teine D, Warrell J, Rosanas-Urgell A, Lin E, Kiniboro B, Vince J, Hwaiwhanje I, Karunajeewa H, Michon P, Siba P, Mueller I, Davis TM: Features and prognosis of severe malaria caused by Plasmodium falciparum Plasmodium vivax and mixed Plasmodium species in Papua New Guinean children. PLoS One. 2011, 6: e29203-10.1371/journal.pone.0029203.PubMed CentralView ArticlePubMed
- Yadav D, Chandra J, Aneja S, Kumar V, Kumar P, Dutta AK: Changing profile of severe malaria in north Indian children. Indian J Pediatr. 2012, 79: 483-487. 10.1007/s12098-011-0603-x.View ArticlePubMed
- Lacerda MV, Mourão MP, Alexandre MA, Siqueira AM, Magalhães BM, Martinez-Espinosa FE, Filho FS, Brasil P, Ventura AM, Tada MS, Couto VS, Silva AR, Silva RS, Alecrim MG: Understanding the clinical spectrum of complicated Plasmodium vivax malaria: a systematic review on the contributions of the Brazilian literature. Malar J. 2012, 11: 12-10.1186/1475-2875-11-12.PubMed CentralView ArticlePubMed
- SVS: Boletim Epidemiológico da Malária. 2012,http://portal.saude.gov.br/portal/arquivos/pdf/boletim_malaria_2010_2011.pdf,
- Cruz-Gallardo I, Diaz-Moreno I, Diaz-Quintana A, Donaire A, Velazquez-Campoy A, Curd RD, Rangachari K, Birdsall B, Ramos A, Holder AA, De la Rosa MA: Antimalarial activity of cupredoxins: the interaction of Plasmodium merozoite surface protein 119 (MSP-119) and Rusticyanin. J Biol Chem. 2013, 288: 20896-20907. 10.1074/jbc.M113.460162.PubMed CentralView ArticlePubMed
- Blackman MJ, Heidrich HG, Donachie S, McBride JS, Holder AA: A single fragment of a malaria merozoite surface protein remains on the parasite during red cell invasion and is the target of invasion-inhibiting antibodies. J Exp Med. 1990, 172: 379-382. 10.1084/jem.172.1.379.View ArticlePubMed
- Holder AA, Blackman MJ, Burghaus PA, Chappel JA, Ling IT, McCallum-Deighton N, Shai S: A malaria merozoite surface protein (MSP1)-structure, processing and function. Mem Inst Oswaldo Cruz. 1992, 87: 37-42.View ArticlePubMed
- Gerold P, Schofield L, Blackman MJ, Holder AA, Schwarz RT: Structural analysis of the glycosyl-phosphatidylinositol membrane anchor of the merozoite surface proteins-1 and -2 of Plasmodium falciparum. Mol Biochem Parasitol. 1996, 75: 131-143. 10.1016/0166-6851(95)02518-9.View ArticlePubMed
- Soares IS, Levitus G, Souza JM, Del Portillo HA, Rodrigues MM: Acquired immune responses to the N- and C-terminal regions of Plasmodium vivax merozoite surface protein 1 in individuals exposed to malaria. Infect Immun. 1997, 65: 1606-1614.PubMed CentralPubMed
- Soares IS, Oliveira SG, Souza JM, Rodrigues MM: Antibody response to the N and C-terminal regions of the Plasmodium vivax merozoite surface protein 1 in individuals living in an area of exclusive transmission of P. vivax malaria in the north of Brazil. Acta Trop. 1999, 72: 13-24. 10.1016/S0001-706X(98)00078-3.View ArticlePubMed
- Park JW, Moon SH, Yeom JS, Lim KJ, Sohn MJ, Jung WC, Cho YJ, Jeon KW, Ju W, Ki CS, Oh MD, Choe K: Naturally acquired antibody responses to the C-terminal region of merozoite surface protein 1 of Plasmodium vivax in Korea. Clin Diagn Lab Immunol. 2001, 8: 14-20.PubMed CentralPubMed
- Rodrigues MH, Cunha MG, Machado RL, Ferreira OC, Rodrigues MM, Soares IS: Serological detection of Plasmodium vivax malaria using recombinant proteins corresponding to the 19-kDa C-terminal region of the merozoite surface protein-1. Malar J. 2003, 2: 39-10.1186/1475-2875-2-39.PubMed CentralView ArticlePubMed
- Morais CG, Soares IS, Carvalho LH, Fontes CJ, Krettli AU, Braga EM: IgG isotype to C-terminal 19 kDa of Plasmodium vivax merozoite surface protein 1 among subjects with different levels of exposure to malaria in Brazil. Parasitol Res. 2005, 95: 420-426. 10.1007/s00436-005-1314-x.View ArticlePubMed
- Wickramarachchi T, Illeperuma RJ, Perera L, Bandara S, Holm I, Longacre S, Handunnetti SM, Udagama-Randeniya PV: Comparison of naturally acquired antibody responses against the C-terminal processing products of Plasmodium vivax merozoite surface protein-1 under low transmission and unstable malaria conditions in Sri Lanka. Int J Parasitol. 2007, 37: 199-208. 10.1016/j.ijpara.2006.09.002.View ArticlePubMed
- Barbedo MB, Ricci R, Jimenez MC, Cunha MG, Yazdani SS, Chitnis CE, Rodrigues MM, Soares IS: Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax. Mem Inst Oswaldo Cruz. 2007, 102: 335-339. 10.1590/S0074-02762007005000040.View ArticlePubMed
- Mourão LC, Morais CG, Bueno LL, Jimenez MC, Soares IS, Fontes CJ, Guimarães Lacerda MV, Xavier MS, Barnwell JW, Galinski MR, Braga EM: Naturally acquired antibodies to Plasmodium vivax blood-stage vaccine candidates (PvMSP-119 and PvMSP-3α359–798 and their relationship with hematological features in malaria patients from the Brazilian Amazon. Microbes Infect. 2012, 14: 730-739. 10.1016/j.micinf.2012.02.011.View ArticlePubMed
- Lima-Junior JC, Rodrigues-da-Silva RN, Banic DM, Jiang J, Singh B, Fabrício-Silva GM, Porto LC, Meyer EV, Moreno A, Rodrigues MM, Barnwell JW, Galinski MR, de Oliveira-Ferreira J: Influence of HLA-DRB1 and HLA-DQB1 alleles on IgG antibody response to the P. vivax MSP-1, MSP-3α and MSP-9 in individuals from Brazilian endemic area. PLoS One. 2012, 7: e36419-10.1371/journal.pone.0036419.PubMed CentralView ArticlePubMed
- Yang C, Collins WE, Sullivan JS, Kaslow DC, Xiao L, Lal AA: Partial protection against Plasmodium vivax blood-stage infection in Saimiri monkeys by immunization with a recombinant C-terminal fragment of merozoite surface protein 1 in block copolymer adjuvant. Infect Immun. 1999, 67: 342-349.PubMed CentralPubMed
- Ling IT, Ogun SA, Holder AA: Immunization against malaria with a recombinant protein. Parasite Immunol. 1994, 16: 63-67.View ArticlePubMed
- Daly TM, Long CA: Humoral response to a carboxyl-terminal region of the merozoite surface protein-1 plays a predominant role in controlling blood-stage infection in rodent malaria. J Immunol. 1995, 155: 236-243.PubMed
- Holder AA, Guevara Patiño JA, Uthaipibull C, Syed SE, Ling IT, Scott-Finnigan T, Blackman MJ: Merozoite surface protein 1, immune evasion, and vaccines against asexual blood stage malaria. Parassitologia. 1999, 41: 409-414.PubMed
- Hirunpetcharat C, Tian JH, Kaslow DC, van Rooijen N, Kumar S, Berzofsky JA, Miller LH, Good MF: Complete protective immunity induced in mice by immunization with the 19-kilodalton carboxyl-terminal fragment of the merozoite surface protein-1 (MSP-119) of Plasmodium yoelii expressed in Saccharomyces cerevisiae: correlation of protection with antigen-specific antibody titer, but not with effector CD4+ T cells. J Immunol. 1997, 159: 3400-3411.PubMed
- Hirunpetcharat C, Stanisic D, Liu XQ, Vadolas J, Strugnell RA, Lee R, Miller LH, Kaslow DC, Good MF: Intranasal immunization with yeast-expressed 19 kD carboxyl-terminal fragment of Plasmodium yoelii merozoite surface protein-1 (yMSP-119) induces protective immunity to blood stage malaria infection in mice. Parasite Immunol. 1998, 20: 413-420. 10.1046/j.1365-3024.1998.00161.x.View ArticlePubMed
- Ichino Y, Ishikawa T: Effects of cryopreservation on human lymphocyte functions: comparison of programmed freezing method by a direct control system with a mechanical freezing method. J Immunol Methods. 1985, 77: 283-290. 10.1016/0022-1759(85)90041-9.View ArticlePubMed
- Schmid I, Uittenbogaart CH, Keld B, Giorgi JV: A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J Immunol Methods. 1994, 170: 145-157. 10.1016/0022-1759(94)90390-5.View ArticlePubMed
- Trager W, Jensen JB: Human malaria parasites in continuous culture. Science. 1976, 193: 673-675. 10.1126/science.781840.View ArticlePubMed
- Riccio EKP, Neves-Júnior I, Riccio LRP, Banic DM, Daniel-Ribeiro CT, Ferreira-da-Cuz MF: Cryopreservation of peripheral blood mononuclear cells does not affect the levels of spontaneous apoptosis after 24h culture. Cryobiology. 2002, 45: 127-134. 10.1016/S0011-2240(02)00121-9.View ArticlePubMed
- Weinberg A, Song LY, Wilkening C, Sevin A, Blais B, Louzao R, Stein D, Defechereux P, Durand D, Riedel E, Raftery N, Jesser R, Brown B, Keller MF, Dickover R, McFarland E, Fenton T: Optimization and limitations of use of cryopreserved peripheral blood mononuclear cells for functional and phenotypic T-cell characterization. Clin Vaccine Immunol. 2009, 16: 1176-1186. 10.1128/CVI.00342-08.PubMed CentralView ArticlePubMed
- Torre D, Speranza F, Giola M, Matteelli A, Tambini R, Biondi G: Role of Th1 and Th2 cytokines in immune response to uncomplicated Plasmodium falciparum malaria. Clin Diagn Lab Immunol. 2002, 9: 348-351.PubMed CentralPubMed
- Quin SJ, Seixas EM, Cross CA, Berg M, Lindo V, Stockinger B, Langhorne J: Low CD4(+) T cell responses to the C-terminal region of the malaria merozoite surface protein-1 may be attributed to processing within distinct MHC class II pathways. Eur J Immunol. 2001, 31: 72-81. 10.1002/1521-4141(200101)31:1<72::AID-IMMU72>3.0.CO;2-Z.View ArticlePubMed
- Mukherjee P, Devi YS, Chauhan VS: Blood stage malaria antigens induce different activation-induced cell death programs in splenic CD4 + T cells. Parasite Immunol. 2008, 30: 497-514. 10.1111/j.1365-3024.2008.01050.x.View ArticlePubMed
- Green DR, Droin N, Pinkoski M: Activation-induced cell death in T cells. Immunol Rev. 2003, 193: 70-81. 10.1034/j.1600-065X.2003.00051.x.View ArticlePubMed
- Ho M, Schollaardt T, Snape S, Looareesuwan S, Suntharasamai P, White NJ: Endogenous interleukin-10 modulates proinflammatory response in Plasmodium falciparum malaria. J Infect Dis. 1998, 178: 520-525. 10.1086/515640.View ArticlePubMed
- Day NP, Hien TT, Schollaardt T, Loc PP, Chuong LV, Chau TT, Mai NT, Phu NH, Sinh DX, White NJ, Ho M: The prognostic and pathophysiologic role of pro- and antiinflammatory cytokines in severe malaria. J Infect Dis. 1999, 180: 1288-1297. 10.1086/315016.View ArticlePubMed
- Walther M, Woodruff J, Edele F, Jeffries D, Tongren JE, King E, Andrews L, Bejon P, Gilbert SC, De Souza JB, Sinden R, Hill AV, Riley EM: Innate immune responses to human malaria: heterogeneous cytokine responses to blood-stage Plasmodium falciparum correlate with parasitological and clinical outcomes. J Immunol. 2006, 177: 5736-5745.View ArticlePubMed
- Li C, Sanni LA, Omer F, Riley E, Langhorne J: Pathology of Plasmodium chabaudi chabaudi infection and mortality in interleukin-10-deficient mice are ameliorated by anti-tumor necrosis factor alpha and exacerbated by anti-transforming growth factor beta antibodies. Infect Immun. 2003, 71: 4850-4856. 10.1128/IAI.71.9.4850-4856.2003.PubMed CentralView ArticlePubMed
- Couper KN, Blount DG, Riley EM: IL-10: the master regulator of immunity to infection. J Immunol. 2008, 180: 5771-5777.View ArticlePubMed
- Rosario AP F d, Langhorne J: T cell-derived IL-10 and its impact on the regulation of host responses during malaria. Int J Parasitol. 2012, 42: 549-555. 10.1016/j.ijpara.2012.03.010.View Article
- O’Donnell RA, Saul A, Cowman AF, Crabb BS: Functional conservation of the malaria vaccine antigen MSP-119across distantly related Plasmodium species. Nat Med. 2000, 6: 91-95. 10.1038/71595.View ArticlePubMed
- Putaporntip C, Jongwutiwes S, Sakihama N, Ferreira MU, Kho WG, Kaneko A, Kanbara H, Hattori T, Tanabe K: Mosaic organization and heterogeneity in frequency of allelic recombination of the Plasmodium vivax merozoite surface protein-1 locus. Proc Natl Acad Sci USA. 2002, 99: 16348-16353. 10.1073/pnas.252348999.PubMed CentralView ArticlePubMed
- Gowda DC: TLR-mediated cell signaling by malaria GPIs. Trends Parasitol. 2007, 23: 596-604. 10.1016/j.pt.2007.09.003.View ArticlePubMed
- McGregor IA, Barr M: Antibody response to tetanus toxoide inoculation in malarious and non-malarious Gambian children. Trans R Soc Trop Med Hyg. 1962, 56: 364-367. 10.1016/0035-9203(62)90005-6.View Article
- Ho M, Webster HK, Looareesuwan S, Supanaranond W, Phillips RE, Chanthavanich P, Warrell DA: Antigen-specific immunosuppression in human malaria due to Plasmodium falciparum. J Infect Dis. 1986, 3: 763-771.View Article
- Oliveira-Ferreira J, Pratt-Riccio LR, Arruda M, Santos F, Ribeiro CT, Goldberg AC, Banic DM: HLA class II and antibody responses to circumsporozoite protein repeats of P. vivax (VK210, VK247 and P. vivax-like) in individuals naturally exposed to malaria. Acta Trop. 2004, 92: 63-69. 10.1016/j.actatropica.2004.02.011.View ArticlePubMed
- Pratt-Riccio LR, Lima-Junior JC, Carvalho LJ, Theisen M, Espindola-Mendes EC, Santos F, Oliveira-Ferreira J, Goldberg AC, Daniel-Ribeiro CT, Banic DM: Antibody response profiles induced by Plasmodium falciparum glutamate-rich protein in naturally exposed individuals from a brazilian area endemic for malaria. Am J Trop Med Hyg. 2005, 73: 1096-1103.PubMed
- Carpenter D, Abushama H, Bereczky S, Färnert A, Rooth I, Troye-Blomberg M, Quinnell RJ, Shaw MA: Immunogenetic control of antibody responsiveness in a malaria endemic area. Hum Immunol. 2007, 68: 165-169. 10.1016/j.humimm.2006.12.002.View ArticlePubMed
- Storti-Melo LM, da Costa DR, Souza-Neiras WC, Cassiano GC, Couto VS, Póvoa MM, Soares Ida S, de Carvalho LH, Arevalo-Herrera M, Herrera S, Rossit AR, Cordeiro JA, de Mattos LC, Machado RL: Influence of HLA-DRB-1 alleles on the production of antibody against CSP, MSP-1, AMA-1, and DBP in Brazilian individuals naturally infected with Plasmodium vivax. Acta Trop. 2012, 121: 152-155. 10.1016/j.actatropica.2011.10.009.View ArticlePubMed
- Sabbagh A, Courtin D, Milet J, Massaro JD, Castelli EC, Migot-Nabias F, Favier B, Rouas-Freiss N, Moreau P, Garcia A, Donadi EA: Association of HLA-G 3′ untranslated region polymorphisms with antibody response against Plasmodium falciparum antigens: preliminary results. Tissue Antigens. 2013, 82: 53-58. 10.1111/tan.12140.View ArticlePubMed
- Jeannin P, Lecoanet S, Delneste Y, Gauchat JF, Bonnefoy JY: IgE versus IgG4 production can be differentially regulated by IL-10. J Immunol. 1998, 160: 3555-3561.PubMed
- Fernandes AA, Carvalho LJ, Zanini GM, Ventura AM, Souza JM, Cotias PM, Silva-Filho IL, Daniel-Ribeiro CT: Similar cytokine responses and degrees of anemia in patients with Plasmodium falciparum and Plasmodium vivax infections in the Brazilian Amazon region. Clin Vaccine Immunol. 2008, 15: 650-658. 10.1128/CVI.00475-07.PubMed CentralView ArticlePubMed
- Cerávolo IP, Bruña-Romero O, Braga EM, Fontes CJ, Brito CF, Souza JM, Krettli AU, Adams JH, Carvalho LH: Anti-Plasmodium vivax duffy binding protein antibodies measure exposure to malaria in the Brazilian Amazon. Am J Trop Med Hyg. 2005, 72: 675-681.PubMed
- Tran TM, Oliveira-Ferreira J, Moreno A, Santos F, Yazdani SS, Chitnis CE, Altman JD, Meyer EV, Barnwell JW, Galinski MR: Comparison of IgG reactivities to Plasmodium vivax merozoite invasion antigens in a Brazilian Amazon population. Am J Trop Med Hyg. 2005, 73: 244-255.PubMed
- Bereczky S, Montgomery SM, Troye-Blomberg M, Rooth I, Shaw MA, Färnert A: Elevated anti-malarial IgE in asymptomatic individuals is associated with reduced risk for subsequent clinical malaria. Int J Parasitol. 2004, 34: 935-942. 10.1016/j.ijpara.2004.04.007.View ArticlePubMed
- Mehrizi AA, Zakeri S, Rafati S, Salmanian AH, Djadid ND: Immune responses elicited by co-immunization of Plasmodium vivax and P. falciparum MSP-1 using prime-boost immunization strategies. Parasite Immunol. 2011, 33: 594-608. 10.1111/j.1365-3024.2011.01331.x.View ArticlePubMed
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