Sex-specific and blood meal-induced proteins of Anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis
© Prévot et al; licensee BioMed Central Ltd. 2003
Received: 15 November 2002
Accepted: 11 February 2003
Published: 11 February 2003
Anopheles gambiae is the main vector of Plasmodium falciparum in Africa. The mosquito midgut constitutes a barrier that the parasite must cross if it is to develop and be transmitted. Despite the central role of the mosquito midgut in the host/parasite interaction, little is known about its protein composition. Characterisation of An. gambiae midgut proteins may identify the proteins that render An. gambiae receptive to the malaria parasite.
We carried out two-dimensional gel electrophoresis of An. gambiae midgut proteins and compared protein profiles for midguts from males, sugar-fed females and females fed on human blood.
Very few differences were detected between male and female mosquitoes for the approximately 375 silver-stained proteins. Male midguts contained ten proteins not detected in sugar-fed or blood-fed females, which are therefore probably involved in male-specific functions; conversely, female midguts contained twenty-three proteins absent from male midguts. Eight of these proteins were specific to sugar-fed females, and another ten, to blood-fed females.
Mass spectrometry analysis of the proteins found only in blood-fed female midguts, together with data from the recent sequencing of the An. gambiae genome, should make it possible to determine the role of these proteins in blood digestion or parasite receptivity.
Anopheles gambiae is the main vector of the human malaria parasite, Plasmodium falciparum, in Africa. The number of cases of malaria, and their severity, have increased, leading to an increase in the social and economic burden of this disease [1, 2]. The development of drug resistance in parasites and insecticide resistance in mosquitoes has contributed to this situation. Malaria incidence could be reduced by controlling parasite transmission by the mosquito. The sporogonic development of Plasmodium, from gamete to oocyst formation, takes place in the lumen and epithelium of the mosquito midgut. Mosquito-specific factors probably determine the outcome of this sporogonic development. Indeed, xanthurenic acid, produced by the mosquito, is important for the exflagellation of parasite microgametes [3, 4]. Trypsin, produced in the mosquito's digestive tract, probably activates parasite chitinase(s), facilitating the passage of the parasite through the peritrophic matrix surrounding the parasite-containing blood meal in the mosquito [5, 6]. Recent studies have shown that early sporogonic stages of Plasmodium parasite modulate the mosquito midgut immune response [7–9]. This suggests that certain immune molecules could be used to inhibit the development of Plasmodium in transgenic mosquitoes [10, 11]. These studies, and others, were based on analyses of mRNA production; very little has been published concerning proteome analysis for mosquito midguts [7–9, 12, 13].
As the early phase of Plasmodium sporogonic development occurs at the same time as blood-meal digestion, the physiology and biochemistry of this process have been extensively studied [14–18]. Indeed, several digestive enzymes secreted within the midgut lumen have been characterised [17, 19–21]. However, very few studies have focused on characterisation of the proteins of the mosquito midgut epithelium. Using monoclonal antibodies, Lal et al.  recently identified a set of midgut proteins that may be involved in Plasmodium development. Ghosh et al.  screened a phage display library and selected a peptide that recognised midgut protein(s), as yet unidentified. The production of this peptide in transgenic Anopheles stephensi mosquitoes reduced the development of Plasmodium berghei oocysts on the mosquito midgut wall .
We analysed the midgut protein profile of female An. gambiae by two-dimensional (2-D) gel electrophoresis. Midguts were isolated 19 h after feeding on uninfected human blood. This time course corresponds to the early phase of ookinete interaction with midgut cells in mosquitoes fed on the blood of gametocyte carriers. We compared the profile obtained with those from the midguts of males and females not fed on blood. We identified a set of proteins that were specifically produced and regulated in females following blood ingestion. The recently determined sequence of the An. gambiae genome could be used in the analysis of these proteins and their potential functions, and transgenesis may provide a new tool for studying the involvement of mosquito proteins in Plasmodium sporogonic development.
Mosquito rearing and blood-feeding
An. gambiae strain G3 was reared at 26°C, in conditions of 80% relative humidity and a 12/12 h light/dark cycle. For mass rearing, female mosquitoes were allowed to feed on the blood of an anaesthetised rabbit. The blood-fed females used in this analysis were first starved for 12 h and then fed on uninfected human blood, using a membrane-feeding device . Unfed or partially fed females were discarded.
All dissections were performed on ice, in PBSI (phosphate-buffered saline containing 1 mM EDTA and 1 mg/ml Pefabloc®). Midguts were dissected from 4-day-old sugar-fed male and female mosquitoes. Midguts were isolated from blood-fed mosquitoes 19 hours after blood-feeding. Midguts from blood-fed mosquitoes were opened by a longitudinal incision and thoroughly rinsed in ice-cold PBSI to remove all traces of peritrophic matrix and gut contents. Dissected midguts were stored at -80°C until processing.
Proteins were extracted as previously described . Briefly, sixty midguts were placed in 150 μl of extraction buffer (0.6% sodium-dodecyl sulphate (SDS), 0.2% β-mercaptoethanol, 10 mM Tris-HCl pH 8.0) supplemented with a cocktail of protease inhibitors (1 μg/ml antipain; 1 μg/ml aprotinin; 1 mM EDTA; 100 μM TPCK; 1 μg/ml leupeptin; 1 mg/ml Pefabloc®; 1 μg/ml pepstatin (Boehringer Mannheim). Samples were homogenized with a Wheaton-33® Potter-Elvehjem tissue grinder, transferred to an Eppendorf tube and boiled for 4 minutes. The samples were centrifuged for 10 minutes at 9,000 g, 4°C, and the supernatant was transferred to a fresh tube. Proteins were concentrated and SDS and β-mercaptoethanol eliminated by precipitation with acetone as follows: 1.5% 1 N NaOH and 9 volumes of ice-cold acetone were added to each protein sample. Samples were incubated for 3 hours or overnight at -20°C and centrifuged for 10 minutes at 9,000 g, 4°C. Pellets were dried for 1 hour under vacuum, using a "speed vac" (Savant) and resuspended in either distilled water, for protein determination, or sample buffer (9.95 M urea; 4% NP-40; 2% ampholines; 100 mM dithiothreitol), for 2-D electrophoresis. For each extract, proteins were precipitated from two separate aliquots: a 30 μl aliquot for protein determination, and a 120 μl aliquot for 2-D electrophoresis.
Protein concentrations were determined in duplicate, using the Lowry-SDS method and microtitre plates [27, 28]. Bovine serum albumin was used as a standard (1 to 20 μg). Absorbance at 630 nm was measured with a Biotech EL 311 spectrophotometer.
Two-dimensional gel electrophoresis was performed essentially as described by Garrels , with the modifications of Laurent-Winter et al. . For the first dimension (isoelectric focusing, IEF), we used gradient gels covering the range pH4 to pH8 (ampholines, Millipore Inc.), which were run for 20,000 Vh. We used 10% acrylamide gels for the second dimension. Gels were silver stained as described by Morrissey . For each sample, 30 μg of protein was run on the gel and molecular weight markers were applied in a separate slot. For each type of midgut (male, sugar-fed female and blood-fed female), at least three independent sample preparations were used and at least three independent gel analyses performed.
Silver-stained gels were examined by eye, using a light box. Protein extracts were compared in pairs by superimposing the corresponding 2-D gels. Differences in the pattern of spots between two extracts were scored if reproduced in at least three independent experiments. We considered only differences involving the presence or absence of a particular spot but differences in the intensities of spots for proteins with identical migration patterns were also observed.
Spots and their corresponding proteins were classified into three categories: a) constitutive – detected in midgut extracts from both sugar-fed and blood-fed females; b) BDP – detected only in blood-fed mosquitoes; c) SDP – detected only in sugar-fed mosquitoes.
Results and Discussion
Sex-specific proteins from An. gambiae midguts
Although midgut morphology differs between male and female mosquitoes, the cellular structure of the midgut is almost identical in males and females, consisting of columnar and regenerative cells [32–34]. The morphological differences may reflect specific functions, and may imply differences at the molecular level.
Both male and female mosquitoes feed on nectar. The ten male-specific proteins are therefore more likely to be involved in male-specific functions or structures than in nectar processing, whereas the eight proteins shared by sugar-fed males and females and absent from blood-fed females are probably involved in sugar processing. In contrast, as male mosquitoes never ingest blood, the BDP detected in the midguts of blood-fed females but not in males are presumably involved in blood digestion, a female-specific function.
Cazares-Raga et al.  analysed the sex-specific proteins and proteases present in the midguts of Anopheles albimanus mosquitoes. They detected approximately 150 well-resolved spots and found that the protein profiles of midguts from males and females differed considerably. The overall profile obtained with midguts from An. albimanus males was similar to that obtained in this study with midguts from An. gambiae males. However, the pattern for An. albimanus females mostly included low-molecular weight proteins. In our experimental conditions, profiles of this type were invariably associated with proteolysis of the sample, resulting in the accumulation of proteins in the lower part of the gel.
Identification of midgut proteins regulated upon blood ingestion
Comparison of protein patterns from sugar-fed and blood-fed female An. gambiae revealed a limited number of differences. Five of the 23 female-specific proteins (Figure 2) were produced in the midguts of both sugar-fed and blood-fed females; these proteins were classified as constitutive proteins. Eight proteins were present only in sugar-fed females (SDP). The ten remaining proteins were present in the midguts of females only after a blood meal (BDP). In addition to differences in the presence/absence of protein spots, we also observed reproducible differences in spot intensity for another small set of proteins. The amount of these proteins either decreased (spots A & B panel A) or increased (spots C-F, panel B) upon blood-feeding.
Proteins specifically produced in blood-fed mosquitoes and proteins overproduced upon blood-feeding are likely to be involved in blood digestion or in synthesis of the peritrophic matrix that surrounds the blood meal. Alternatively, some of these proteins may be involved in defence reactions directed against micro-organisms ingested along with the blood meal. We conducted our analysis 19 h after feeding. At this time, Plasmodium ookinetes interact with midgut cells if the mosquito fed on Plasmodium gametocyte-containing blood; some of the BDP may therefore be involved in host-parasite interactions as well. Indeed, in a well-adapted host-parasite system, such as that constituted by P. falciparum and An. gambiae, the parasite probably makes use of pre-existing host molecules to ensure its development at a lower cost to the host. If this is indeed the case, then some of the BDP identified here may be of relevance to P. falciparum/An. gambiae midgut interactions.
In previous studies of differences between An. stephensi strains refractory and susceptible to infection by P. falciparum, we also detected sugar-meal and blood-meal-dependent proteins in female mosquitoes . The main difference between the refractory and susceptible An. stephensi lines was the larger number of BDP in the susceptible line. This observation is consistent with the possible involvement of some midgut BDP in Plasmodium/ Anopheles midgut interactions.
Overall, in found 16 SDP in the midguts of An. gambiae females: eight were female-specific and the other eight were present in both males and females. We also found ten BDP. All the BDP were female-specific, as males do not feed on blood. The characterisation of BDP by mass spectrometry of protein spots isolated from gels will be greatly facilitated by the recent completion of the whole genome sequence of An. gambiae . This will undoubtedly provide additional information as to whether these proteins are involved in blood digestion or in the development of the malaria parasite. The characterisation of female-specific proteins may also be useful for the identification of blood meal-inducible, female-specific promoters, which could be used to drive the expression of genes encoding proteins impairing the development of Plasmodium parasites in transgenic mosquitoes .
We would like to thank Jean-Claude Jacques for technical assistance and Nadia Monnier for rearing mosquitoes. This work was supported in part by fellowships awarded to G.P. (MRE, FRM and CRG) and research funds from the Pasteur Institute and the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR).
- Greenwood B, Mutabingwa T: Malaria in 2002. Nature. 2002, 415: 670-2. 10.1038/415670a.View ArticlePubMedGoogle Scholar
- Sachs J, Malaney P: The economic and social burden of malaria. Nature. 2002, 415: 680-5. 10.1038/415680a.View ArticlePubMedGoogle Scholar
- Billker O, Lindo V, Panico M, Etienne AE, Paxton T, Dell A, Rogers M, Sinden RE, Morris HR: Identification of xanthurenic acid as the putative inducer of malaria development in the mosquito. Nature. 1998, 392: 289-292. 10.1038/32667.View ArticlePubMedGoogle Scholar
- Garcia GE, Wirtz RA, Rosenberg R: Isolation of a substance from the mosquito that activates Plasmodium fertilization. Mol Biochem Parasitol. 1997, 88: 127-135. 10.1016/S0166-6851(97)00086-8.View ArticlePubMedGoogle Scholar
- Shahabuddin M, Kaslow DC: Plasmodium: Parasite chitinase and its role in malaria transmission. Exp Parasitol. 1994, 79: 85-88. 10.1006/expr.1994.1066.View ArticlePubMedGoogle Scholar
- Shahabuddin M, Lemos FJA, Kaslow DC, Jacobslorena M: Antibody-mediated inhibition of Aedes aegypti midgut trypsins blocks sporogonic development of Plasmodium gallinaceum. Infect Immun. 1996, 64: 739-743.PubMed CentralPubMedGoogle Scholar
- Dimopoulos G, Seeley D, Wolf A, Kafatos FC: Malaria infection of the mosquito Anopheles gambiae activates immune-responsive genes during critical transition stages of the parasite life cycle. EMBO J. 1998, 17: 6115-6123. 10.1093/emboj/17.21.6115.PubMed CentralView ArticlePubMedGoogle Scholar
- Dimopoulos G, Muller H, Levashina EA, Kafatos FC: Innate immune defense against malaria infection in the mosquito. Curr Opin Immunol. 2001, 13: 79-88. 10.1016/S0952-7915(00)00186-2.View ArticlePubMedGoogle Scholar
- Tahar R, Boudin C, Thiery I, Bourgouin C: Immune response of Anopheles gambiae to the early sporogonic stages of the human malaria parasite, Plasmodium falciparum. EMBO J. 2002, 21: 6673-6680. 10.1093/emboj/cdf664.PubMed CentralView ArticlePubMedGoogle Scholar
- Kokoza V, Ahmed A, Cho WL, Jasinskiene N, James AA, Raikhel A: Engineering bloodmeal-activated systemic immunity in the yellow fever mosquito, Aedes aegypti. Proc Natl Acad Sci U S A. 2000, 97: 9144-9. 10.1073/pnas.160258197.PubMed CentralView ArticlePubMedGoogle Scholar
- Raikhel AS, Kokoza VA, Zhu J, Martin D, Wang S-F, Li C, Sun G, Ahmed A, Dittmer N, Attardo G: Molecular biology of mosquito vitellogenesis: from basic studies to genetic engineering of antipathogen immunity. Insect Biochem Mol Biol. 2002, 32: 1275-1286. 10.1016/S0965-1748(02)00090-5.View ArticlePubMedGoogle Scholar
- Shen Z, Dimopoulos G, Kafatos FC, Jacobs-Lorena M: A cell surface mucin specifically expressed in the midgut of the malaria mosquito Anopheles gambiae. Proc Natl Acad Sci U S A. 1999, 96: 5610-5. 10.1073/pnas.96.10.5610.PubMed CentralView ArticlePubMedGoogle Scholar
- Oduol F, Xu JN, Niare O, Natarajan R, Vernick KD: Genes identified by an expression screen of the vector mosquito Anopheles gambiae display differential molecular immune response to malaria parasites and bacteria. Proc Natl Acad Sci U S A. 2000, 97: 11397-11402. 10.1073/pnas.180060997.PubMed CentralView ArticlePubMedGoogle Scholar
- Billingsley PF, Hecker H: Blood digestion in the mosquito Anopheles stephensi Liston (Diptera: Culicidae): activity and distribution of trypsin, aminopeptidase and α-glucosidase in the midgut. J Med Entomol. 1991, 28: 865-871.View ArticlePubMedGoogle Scholar
- Chadee DD, Beier JC: Blood-digestion kinetics of four Anopheles species from Trinidad, West Indies. Ann Trop Med Parasitol. 1995, 89: 531-540.PubMedGoogle Scholar
- Chege GM, Beier JC: Blood acquisition and processing by three Anopheles (Diptera: Culicidae) species with different innate susceptibilities to Plasmodium falciparum. J Med Entomol. 1998, 35: 319-23.View ArticlePubMedGoogle Scholar
- Clements AN: The biology of mosquitoes. London: Chapman and Hall. 1992Google Scholar
- Lehane MJ, Billingsley PF: The Insect Midgut. Chapman and Hall. 1996Google Scholar
- Graf R, Raikhel AS, Brown MR, Lea AO, Briegel H: Mosquito trypsin: immunocytochemical localisation in the midgut of blood-fed Aedes aegypti (L.). Cell Tissue Res. 1986, 245: 19-27.View ArticlePubMedGoogle Scholar
- Muller HM, Vizioli I, della Torre A, Crisanti A: Temporal and spatial expression of serine protease genes in Anopheles gambiae. Parassitologia. 1993, 35: 73-6.PubMedGoogle Scholar
- Müller HM, Crampton JM, Dellatorre A, Sinden R, Crisanti A: Members of a trypsin gene family in Anopheles gambiae are induced in the gut by bloodmeal. EMBO J. 1993, 12: 2891-2900.PubMed CentralPubMedGoogle Scholar
- Lal AA, Patterson PS, Sacci JB, Vaughan JA, Paul C, Collins WE, Wirtz RA, Azad AF: Anti-mosquito midgut antibodies block development of Plasmodium falciparum and Plasmodium vivax in multiple species of Anopheles mosquitoes and reduce vector fecundity and survivorship. Proc Natl Acad Sci U S A. 2001, 98: 5228-33. 10.1073/pnas.091447398.PubMed CentralView ArticlePubMedGoogle Scholar
- Ghosh AK, Ribolla PE, Jacobs-Lorena M: Targeting Plasmodium ligands on mosquito salivary glands and midgut with a phage display peptide library. Proc Natl Acad Sci U S A. 2001, 98: 13278-81. 10.1073/pnas.241491198.PubMed CentralView ArticlePubMedGoogle Scholar
- Ito J, Ghosh A, Moreira LA, Wimmer EA, Jacobs-Lorena M: Transgenic anopheline mosquitoes impaired in transmission of a malaria parasite. Nature. 2002, 417: 452-5. 10.1038/417452a.View ArticlePubMedGoogle Scholar
- Tchuinkam T, Mulder B, Dechering K, Stoffels H, Verhave JP, Cot M, Carnevale P, Meuwissen JHET, Robert V: Experimental infections of Anopheles gambiae with Plasmodium falciparum of naturally infected gametocyte carriers in Cameroon – factors influencing the infectivity to mosquitoes. Trop Med Parasitol. 1993, 44: 271-276.PubMedGoogle Scholar
- Prévot GI, Laurent-Winter C, Feldmann AM, Rodhain F, Bourgouin C: Two-dimensional gel analysis of midgut proteins of Anopheles stephensi lines with different susceptibility to Plasmodium falciparum infection. Insect Mol Biol. 1998, 7: 375-383. 10.1046/j.1365-2583.1998.740375.x.View ArticlePubMedGoogle Scholar
- Harrington CR: Lowry protein assay containing sodium dodecyl sulfate in microtiter plates for protein determinations on fractions from brain tissue. Anal Biochem. 1990, 186: 285-287.View ArticlePubMedGoogle Scholar
- Markwell MAK, Haas SM, Bieber LL, Tolbert NE: A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978, 87: 206-210.View ArticlePubMedGoogle Scholar
- Garrels JI: Quantitative two-dimensional gel electrophoresis of proteins. Meth Enzymol. 1983, 100: 411-423.View ArticlePubMedGoogle Scholar
- Laurent-Winter C, Fougère-Deschatrette C, Weiss MC: Identification of polypeptides whose presence correlates with retention or loss of an albumin extinguisher chromosome in rat hepatoma-mouse L cell fibroblast microcell hybrids. Differentiation. 1994, 55: 225-232. 10.1046/j.1432-0436.1994.5530225.x.View ArticlePubMedGoogle Scholar
- Morrissey JH: Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981, 117: 307-310.View ArticlePubMedGoogle Scholar
- Hecker H, Freyvogel TA, Briegel H, Steiger R: The ultrastructure of midgut epithelium in Aedes aegypti L. (Insecta, Diptera) males. Acta Trop. 1971, 28: 275-290.PubMedGoogle Scholar
- Hecker H, Freyvogel TA, Briegel H, Steiger R: Ultrastructural differentiation of the midgut epithelium in female Aedes aegytpi L. (Insecta, Diptera) imagines. Acta Trop. 1971, 28: 80-104.PubMedGoogle Scholar
- Hecker H: Structure and function of midgut epithelial cells in Culicidae mosquitoes (Insecta, Diptera). Cell Tissue Res. 1977, 184: 321-341.View ArticlePubMedGoogle Scholar
- Cazares-Raga FE, Sanchez-Contreras ME, Rodriguez MH, Hernandez-Hernandez FC: Sex-specific proteins and proteases present in the midguts of Anopheles albimanus (Diptera: Culicidae). J Med Entomol. 1998, 35: 184-6.View ArticlePubMedGoogle Scholar
- Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R: The genome sequence of the malaria mosquito Anopheles gambiae. Science. 2002, 298: 129-49. 10.1126/science.1076181.View ArticlePubMedGoogle Scholar
- Grossman GL, Rafferty CS, Clayton JR, Stevens TK, Mukabayire O, Benedict MQ: Germline transformation of the malaria vector, Anopheles gambiae, with the piggyBac transposable element. Insect Mol Biol. 2001, 10: 597-604. 10.1046/j.0962-1075.2001.00299.x.View ArticlePubMedGoogle Scholar
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