Longitudinal survey of malaria morbidity over 10 years in Saharevo (Madagascar): further lessons for strengthening malaria control
- Léon P Rabarijaona†1, 2,
- Milijaona Randrianarivelojosia†1Email author,
- Lucie A Raharimalala1, 3,
- Arsène Ratsimbasoa1,
- Arthur Randriamanantena1,
- Laurence Randrianasolo1,
- Lanto A Ranarivelo1, 4,
- Fanja Rakotomanana1,
- Rindra Randremanana1,
- Jocelyn Ratovonjato1,
- Marie-Ange Rason1,
- Jean-Bernard Duchemin1, 5,
- Adama Tall1, 6,
- Vincent Robert1, 7,
- Ronan Jambou1, 8,
- Frédéric Ariey1, 9 and
- Olivier Domarle1
© Rabarijaona et al; licensee BioMed Central Ltd. 2009
Received: 21 January 2009
Accepted: 6 August 2009
Published: 6 August 2009
Madagascar has been known for having bio-geo-ecological diversity which is reflected by a complex malaria epidemiology ranging from hyperendemic to malaria-free areas. Malaria-related attacks and infection are frequently recorded both in children and adults living in areas of low malaria transmission. To integrate this variability in the national malaria control policy, extensive epidemiological studies are required to up-date previous records and adjust strategies.
A longitudinal malaria survey was conducted from July 1996 to June 2005 among an average cohort of 214 villagers in Saharevo, located at 900 m above the sea. Saharevo is a typical eastern foothill site at the junction between a costal wet tropical area (equatorial malaria pattern) and a drier high-altitude area (low malaria transmission).
Passive and active malaria detection revealed that malaria transmission in Saharevo follows an abrupt seasonal variation. Interestingly, malaria was confirmed in 45% (1,271/2,794) of malaria-presumed fevers seen at the health centre. All four Plasmodia that infect humans were also found: Plasmodium falciparum; Plasmodium vivax, Plasmodium malariae and Plasmodium ovale. Half of the malaria-presumed fevers could be confirmed over the season with the highest malaria transmission level, although less than a quarter in lower transmission time, highlighting the importance of diagnosis prior to treatment intake. P. falciparum malaria has been predominant (98%). The high prevalence of P. falciparum malaria affects more particularly under 10 years old children in both symptomatic and asymptomatic contexts. Children between two and four years of age experienced an average of 2.6 malaria attacks with P. falciparum per annum. Moreover, estimated incidence of P. falciparum malaria tends to show that half of the attacks (15 attacks) risk to occur during the first 10 years of life for a 60-year-old adult who would have experienced 32 malaria attacks.
The incidence of malaria decreased slightly with age but remained important among children and adults in Saharevo. These results support that a premunition against malaria is slowly acquired until adolescence. However, this claims for a weak premunition among villagers in Saharevo and by extension in the whole eastern foothill area of Madagascar. While the Malagasy government turns towards malaria elimination plans nowadays, choices and expectations to up-date and adapt malaria control strategies in the foothill areas are discussed in this paper.
In Madagascar island, a broad range of malaria transmission patterns coexist following the local bio-geo-ecological diversity. In coastal areas, high levels of malaria transmission prompt the development of acquired immunity [1–3]. Even though life-threatening malaria such as cerebral malaria is relatively rare in these regions, the incidence of severe anaemia is high and especially affects children. In contrast, seasonal and low malaria transmission pattern are typical of highland plateaus located at 1,000 to 1,500 meters above the sea. Acquired immunity is low there and malaria affects all age groups [4, 5]. Between highlands and coasts, the complexity of malaria transmission patterns increases. Depicted as an autonomous transmission area, intermediate zones might be responsible of parasites re-invasion in the highlands. Unfortunately, epidemiologic characteristics remain poorly understood in these areas although understanding and controlling the malaria parasite transmission there appear crucial to eliminate malaria in Madagascar. To get a more accurate definition of malaria transmission in such intermediate areas, an epidemiological study was performed among the villagers of Saharevo. Data collected from July 1996 to June 2005 were the basis of an extensive study giving rise to an in-depth local surveillance reported herein which also highlights key points to define and adapt malaria control strategies.
Biogeography and malariometric parameters at study site
Saharevo annual population census between 1996 and 2005 rose from 182 to 261 inhabitants, with a gap of data in 2002 due to post-electoral turmoil. A mean of 214 inhabitants per annum was estimated and on average half of them were less than 15 years old. This is typical of developing countries and of Madagascar. Due to regular neighborhood communities visits at Saharevo, a total of 325 participants were included in this study. Two main ethnic groups, Bezanozano and Merina, coexist in the village and form 43 families distributed among 39 houses built according to the traditional style of mud walls and thatched roofs. Major activities rely on charcoal production and agricultural works: essentially rice culture during the rainy season from November to April, coupled with local production of cassava, peanuts, sweet potatoes, and garden vegetables. Small herds of domestic animals grew near the villagers. About 50 beef cattle lived in enclosures at the vicinity of houses while fowl roamed free in the daytime though kept home, usually in the kitchen, over night. Short time adult migrations to areas with distinct malaria transmission compared to Saharevo profile regularly occurred but without any defined frequency.
Clinical and parasitological monitoring
Villagers were enrolled to the survey on a consent basis. After the first round census, a unique code number was attributed to any participant for basic demographic and socio-economic indicators to be later confronted with the clinical and biological data that would be continuously collected during the project. The follow-up consisted in malaria case detection on a monthly active basis among villagers and in a passive way among outpatients visiting the dispensary, where a physician was present 24 h a day, five days a week. The clinical signs and symptoms associated with malaria attacks were systematically recorded following an ad hoc questionnaire and recorded in patient personal folders. Each examination was completed with measurement of axillary temperatures with digital thermometers and collection of blood smears from finger pricks.
At each visit, fingerpick blood samples were collected for parasitological checking. Thin and thick smears were carried out on glass slides and stained with Giemsa. Two samples of blood smears were systematically made for each patient. In case of malaria suspicion, one slide was immediately examined to confirm or not the diagnosis. All blood smears were examined by experienced physicians or technicians. Plasmodium falciparum densities were calculated after counting asexual and sexual parasites among 1,000 leukocytes, further converted into parasites load (trophozoits and/or gametocytes) per microliter of blood. Assuming an average leukocyte count of 8,000/μl (WHO, 1991), the detection threshold was about eight parasites/μl of blood. The gametocytes density was separately recorded. Blood smears were stored at the Intitut Pasteur de Madagascar in Antananarivo for quality control purposes.
Passive malaria detection
Microscopic passive malaria detection was done at the primary health centre at Saharevo in any patients with fever (axillary temperature ≥37.5°C) or reporting a recent fever history. Presence of asexual Plasmodium confirmed malaria attack. In that case, patient were given a chloroquine treatment – as front line treatment – in agreement with the national malaria control policy guidelines [6–8]. Chloroquine was administered under physician supervision at 25 mg/kg over three days (10 mg/kg during the two first days and 5 mg/kg the third day). In 2005, chloroquine was left and amodiaquine was used at 10 mg/kg/day over three days. Paracetamol was simultaneously administered for three days at 50 mg/kg/day. Malaria attacks recurring within 31 days were considered as recrudescent infections and treated with the second line treatment (sulphadoxine-pyrimethamine). When microscopy did not confirm malaria or in case of non-malaria pathologies, symptomatic or specific treatment was administered according to the national health policy. Finally, the population was asked to report to the physician any other drug intakes.
Active malaria detection
Every study participant underwent a monthly examination including measurement of axillary temperature and blood smears collection for active malaria detection by microscopy. Interest of systematic screening relies on the surveillance of both symptomatic and asymptomatic parasite carriers. Patients with presence of asexual parasites in blood but without fever (axillary temperature <37.5°C) were reported asymptomatic carriers. Data were collected from February 2003 to June 2005.
Data management and analysis
Data were double-entered into Microsoft Access® version 2.6 databases (Microsoft Corporation) and completeness and internal integrity checks performed on a weekly basis. Analysis was done using SPSS® version 10.0 (Stata Corp., College Station, TX, USA) according to a pre-agreed analytical plan. Statistical significance was set at P < 0.05.
Authorizations to carry out malaria studies in Saharevo were delivered by the Ministry of Health and Family Planning (MoH), the Regional Direction of Health in Mangoro, the Health District in Moramanga, and by local administrative officials. Ethical clearance was obtained from the national ethical committee. Also, to ensure and optimize villagers' commitment to the project, information meetings were regularly held in Saharevo providing explanation related to the protocol and the objectives of this study in Malagasy language. Individual informed consent was obtained from each participant or legal guardians for children. Participation in the project was on a voluntary basis, and all villagers were eligible without exclusion criterion. Primary health care was provided free of charge to everyone, including those who did not join the cohort. The Institut Pasteur de Madagascar is among the major technical partners of the MoH. Saharevo is part of the national network for the surveillance of malaria parasite resistance to drugs – a network known as RER for Réseau d'Etude de la Résistance, recognized by the MoH since 1999 [9–11].
High seasonality of malaria infection in Saharevo
Seasonal fluctuations of P. falciparum malaria rates were recorded in both children and adults. However several observations tend toward that seasonality might be even more marked in children: (i) in children <15 years, P. falciparum malaria attack rate dropped from 55.1% over January – July to 31.6% in August – December (χ2 test p < 0.01); although (ii) in adults ≥15 years old, it only dropped from 35.2% to 19.7% over the same time frames (χ2 test p < 0.01). Interestingly, men had significantly much more P. falciparum malaria attacks than women with 47.7% confirmed malaria cases vs 41.1% respectively (χ2 test p < 0.001). Nevertheless, no sex related difference has been reported for annual incidence (mean annual incidences for men and women: 38.6 ± 13.3% and 33.1 ± 12% respectively).
P. falciparum malaria attacks in Saharevo children
Finally, as long as the medical team was on site – a team from the Institut Pasteur de Madagascar – no death attributed to malaria was recorded. Unfortunately, three children deaths have been reported in 2002, during the post-electoral turmoil, while medical assistance and project conduction had to be temporary suspended. Verbal autopsy revealed that those children suffered from strong fevers and convulsions.
P. falciparum in asymptomatic infections
Interestingly this shift disappeared in 2005, when chloroquine was replaced by amodiaquine and when asymptomatic malaria cases where systematically treated, giving rise to the complete overlapping of asymptomatic carriage rate and attacks incidence (Figure 4).
The tight relationships existing between malaria epidemiology and land altitude have been extensively studied both in mountain and costal regions [12, 13]. In this Saharevo project, the longitudinal study performed among villagers described with accuracy the seasonality and the intensity on P. falciparum infection there. This survey, based on the analysis of data collected over 10 years, enables in-depth determination of malaria epidemiology in areas of intermediate altitude in order to set up adapted strategy in accordance with current malaria elimination policies in Madagascar.
Introducing a vector control plan in the eastern foothills of Madagascar
Saharevo is located in an intermediate zone in between coastal areas with high-transmission equatorial malaria and highland areas with unstable malaria transmission but malaria epidemic risks . In term of malaria epidemiological patterns, this village displays mesoendemic malaria. Strong seasonal variations of malaria incidence indicate that malaria transmission increases from January – when rainfalls are maximal, and peaks in April – coincident with rainy season completion, before getting back close to zero level. Coincidence between transmission peak and rainy season is related to vectors density and behavior. This is supported by the fact that April is also the season of paddies water-filling, multiplying surfaces for hosting An. funestus breeding sites , the major malaria vector in Saharevo. Thus, altogether, rice field proximity, altitude, and economic status influence malaria prevalence in surrounding communities [15–17].
Before and during the time of this project, no interventions to control vectors have been conducted in the region, since this was not part of the national the national policy for foothills areas at that time. The annual EIR in Saharevo was estimated at 2.78 in 2004 and this indicates a low malaria transmission in the foothills areas of Madagascar, at least when compared to EIR in coastal areas in Madagascar or in other areas in mainland Africa [18, 19]. By contrast, despite the weak transmission, parasitological data presented herein are consistent with a relatively high level of malaria endemicity in Saharevo (Figure 2). As in many rural areas in Madagascar, the poor quality of traditional houses in Saharevo promotes the in and out journey of mosquitoes at ease, especially since no vector control measures have ever been implemented in this zone for more than a decade including the project period. On top of that, the villagers currently display a low immunity against malaria; hence most of mosquito bites are likely to lead to an infection (malaria with or without clinical signs). From our previous study carried out in Mahasolo, located in the western foothills area of Madagascar, a similar high malaria endemicity was also described despite a low malaria transmission level . This particular malaria epidemiological profile in the foothills of Madagascar could be controlled by use of indoor spraying of insecticide and insecticide treated bed nets. Moreover, the success of the vector control in the foothills areas in Madagascar has been proven and admitted since the 1950s [7, 21]. Thus, pursuing efforts in vector control activities will significantly help reducing malaria transmission intensity, malaria incidence and malaria prevalence among population in Saharevo in particular, and in extended foothill areas in general. Therefore, it is clearly stated in the national strategy for malaria control revised in April 2009 that all activities will be strengthened from 2009 to 2012 to achieve malaria elimination in Madagascar. Scaling-up the vector control based on the use of insecticide-treated bed nets and the indoor spraying of insecticides is on top priority.
Introducing new malaria case management in the eastern foothills of Madagascar
Until the final year of the project, P. falciparum was predominant in Saharevo (98% of malaria cases). Chloroquine has been used as the first line treatment and the failure rate to this treatment was more than 15% as reported herein or elsewhere [7, 22, 23]. Even though there was no early treatment failure and mainly late parasitological failures that occurred after day 7, this indicates a limited therapeutic efficacy of this drug. It is only in 2005 that amodiaquine was used. Amodiaquine has revealed its high efficacy in this study which has been used as a reference to lead changing in treatment policy in Madagascar .
The incidence of malaria attacks was the highest in two to four years old children although the prevalence of asymptomatic carriers preferentially affected the five to nine years old group. Before amodiaquine introduction, prevalence of asymptomatic carriers tent to peak three months later than incidence of malaria attacks. This supports that a former malaria attack offers a better tolerance to face further infections. When amodiaquine was used to treat malaria infection in Saharevo, this shift immediately disappeared simultaneously with a minor proportion of asymptomatic carriers (Figure 4), although no significant reduction of malaria incidence could be really observed. This highlights the rapid effect on malariometric parameters following a changing in drug policy. The importance of adopting an efficient treatment was also illustrated. The benefits of amodiaquine use will be definitely implemented with the deployment of the artemisinin-based combination therapy artesunate + amodiaquine. It is worth mentioning that recent articles report how efficacy this combination is, especially in Madagascar [24, 25].
From these observations, drugs clearly play a pivotal role to control malaria and especially to turn towards malaria elimination in Madagascar. Interest of introducing the artesunate + amodiaquine combination relies also on its gametocytocide activity. While 86% of gametocyte carriers were < 15 year old children, forming the main parasite reservoir in Saharevo, such a policy would have an impact on malaria transmission especially in children. At the community level – especially in remote villages in foothill areas, replacing pre-packaged chloroquine by the artesunate + amodiaquine formulation for in-home treatment during rainy season should be a successful approach to ensure an efficient reduction of malaria transmission.
Improving malaria diagnosis in the eastern foothills of Madagascar
In Saharevo, almost 10% of malaria attacks occur with very low parasite load less than 200 parasites/μl. Nevertheless, co-infections with viruses or other pathogens might simultaneously occur. The parasite density required to distinguish malaria attacks from other fever causes is unclear in the context of low transmission intensity . Moreover, diagnosing co-infections and establishing pyrogenic thresholds would be strongly compromised by both practical and public health issues anyway. Most of Malagasy primary health centers can not run diagnosis for bacterial or viral infections due to a lack of equipments and resources. Also, practically, from a public health angle, there is a limited interest to determine malaria pyrogenic threshold especially in regions with seasonal transmission as it is in Saharevo and in Madagascar generally. This point is particularly relevant in the context of malaria elimination policy. Since the transition period preceding proper malaria elimination is expected to be marked by a drop of malaria prevalence and incidence, an increased proportion of malaria attacks with low parasitaemia are expected among the remaining cases both in children and adults, due to the loss and the absence of acquired immunity in the population. Then, Plasmodium sp tracking and screening have to become fundamental aspects for achieving malaria elimination, whilst any malaria case detected has to be treated irrespective of the parasitaemia. This is expected to contribute to the reduction of malaria transmission.
During the malaria transmission season (January – July), almost half of the malaria-presumed fevers were confirmed cases of malaria attacks by microscopy in Saharevo. This even dropped to less than a quarter of them during the low malaria transmission season (August – December). On that basis, in the integrated management of childhood illnesses at the community level or at a heath centres that do not use malaria biological diagnosis, more than half of the cases of fevers may be treated abusively with antimalarial. Providing tools for biological diagnosis of malaria to discriminate non-malaria cases will be crucial to develop case management programs in areas with a malaria epidemiologic context similar to that of Moramanga where Saharevo is. Microscopy is a better choice for health facilities, but rapid diagnostic test can be routinely used at the health facilities and at the community level. With regards to rapid diagnostic test, from recent experiences in Madagascar, detecting pLDH will be helpful for this purpose  since the remanence of pfHRP will increase the proportion of the false positive results .
Towards the application of malaria elimination and the strengthening of malaria control strategies in Madagascar
In Madagascar, importance of studying malaria epidemiology in foothills located at 800 to 1,000 m above the sea relies on that a third of the Malagasy population (approximately 6,623,800 inhabitants) live nowadays in such area. In accordance with the major results of our studies, the MoH plans to extend the vector control to the foothills. Malaria endemicity in intermediate areas is then expected to drop and foothills will progressively become regions prone to malaria epidemics, closer to highlands. This may imply changing in malaria trends while children under five and pregnant women will not be the only vulnerable groups. In this context, lessons from the past would help to adjust strategies in response to the malaria epidemiology evolution. As mentioned above, former success of malaria prevention through DDT indoor spraying occurred between 1940s and 1960s, especially in the highlands and the foothill areas in Madagascar [7, 21]. The sudden interruption of the DDT indoor spraying was a lot responsible of the fatal malaria epidemic in the 1980s that triggered deaths mainly in these regions [7, 29]. From this gloomy experience is highlighted how crucial it is to keep sustaining both cure and prevention to combat malaria. Madagascar can also learn from the experiences in other countries that reached a significant malaria reduction for making malaria policy change successful [30–33].
In Saharevo, malaria transmission is seasonal. The incidence of malaria is important among children but slightly decreases with age. These results support that a premunition against malaria is slowly acquired until adolescence although this premunition remains weak premunition in villagers and by extension in the whole eastern foothill area of Madagascar.
From a public health angle, the Saharevo experience nicely illustrates once more that an adequate malaria care, with a confirmed diagnosis and early effective treatment prevent deaths. This must be routinely extended to the whole foothills and to the entire country in Madagascar. These foothill areas are potentially considered as "the entrance gate" of malaria parasites from low land (with high transmission) to highlands (with low transmission). For instance, the use of fixed-dose combination of artesunate + amodiaquine to treat malaria in the primary health centres, improved community case management of malaria in remote areas – which has been already part of the Malagasy malaria control policy , combined with an active vector control plan, should be the efficient partners to tackle directly malaria endemicity in the foothills and indirectly epidemics in the highlands. As soon as vector control will be initiated, regular surveys will be fundamental (i) to up-date malariometric indexes including parasite rate among villagers, uncomplicated malaria cases at the primary health centres, and severe malaria trends at the hospitals; and (ii) to adjust the local strategies for an optimal application of the national policy.
The authors are grateful to the villagers of Saharevo for their participation in the project, and thank the medical team that performed the field work, the Service de Santé de District de Moramanga, and the Malagasy Programme National de Lutte contre le Paludisme for their active involvement in the project. Access to primary health care is of first importance and decent structures are sorely lacking in rural areas in Madagascar. In 2008, as a support to the Moramanga Service de Santé de District, the Institut Pasteur de Madagascar invested in building and bringing new equipments to a modern primary health centre in Saharevo to host medical staff managed by the Ministry of Health and Family Planning. The authors are also grateful to Dr Catherine Martin for her critical reading of the manuscript. This work was financially supported by the French Government via the Académie des Sciences grants (prix Fondation Louis D) and the FSP/RAI project; the International Atomic Energy Agency RAF 6/025, the Institut Pasteur de Madagascar and partly by the Global Fund to Fight AIDS, Tuberculosis and Malaria round 3 grant MDG-304-G05-M.
- Mouchet J, Blanchy S, Rakotonjanabelo A, Ranaivoson G, Rajaonarivelo E, Laventure S, Rossella M, Aknouche F: Stratification épidémiologique du paludisme à Madagascar. Arch Inst Pasteur Madagascar. 1993, 60: 50-59.PubMed
- Albonico M, De Giorgi F, Razanakolona J, Raveloson A, Sabatinelli G, Pietra V, Modiano D: Control of epidemic malaria on the highlands of Madagascar. Parassitologia. 1999, 41: 373-376.PubMed
- Quakyi IA, Leke RG, Befidi-Mengue R, Tsafack M, Bomba-Nkolo D, Manga L, Tchinda V, Njeungue E, Kouontchou S, Fogako J, Nyonglema P, Harun LT, Djokam R, Sama G, Eno A, Megnekou R, Metenou S, Ndountse L, Same-Ekobo A, Alake G, Meli J, Ngu J, Tietche F, Lohoue J, Mvondo JL, Wansi E, Leke R, Folefack A, Bigoga J, Bomba-Nkolo C, Titanji V, Walker-Abbey A, Hickey MA, Johnson AH, Taylor DW: The epidemiology of Plasmodium falciparum malaria in two Cameroonian villages: Simbok and Etoa. Am J Trop Med Hyg. 2000, 63: 222-230.PubMed
- Snow RW, Omumbo JA, Lowe B, Molyneux CS, Obiero JO, Palmer A, Weber MW, Pinder M, Nahlen B, Obonyo C, Newbold C, Gupta S, Marsh K: Relation between severe malaria morbidity in children and level of Plasmodium falciparum transmission in Africa. Lancet. 1997, 349: 1650-1654. 10.1016/S0140-6736(97)02038-2.View ArticlePubMed
- Jambou R, Ranaivo L, Raharimalala L, Randrianaivo J, Rakotomanana F, Modiano D, Pietra V, Boisier P, Rabarijaona L, Rabe T, Raveloson N, De Giorgi F: Malaria in the highlands of Madagascar after five years of indoor house spraying of DDT. Trans Roy Soc Trop Med Hyg. 2001, 95: 14-18. 10.1016/S0035-9203(01)90317-7.View ArticlePubMed
- Milijaona R, Raharimalala L, Ramambanirina L, Ranaivo LH, Jambou R: Drug resistance of Plasmodium falciparum along the borders of the highlands in Madagascar: outlook for a national control program. Med Trop. 1998, 58: 261-265.
- Randrianarivelojosia M, Raveloson A, Randriamanantena A, Juliano JJ, Andrianjafy T, Raharimalala LA, Robert V: Lessons learnt from the six decades of chloroquine use (1945–2005) to control malaria in Madagascar. Trans R Soc Trop Med Hyg. 2009, 103: 3-10. 10.1016/j.trstmh.2008.09.013.View ArticlePubMed
- Rason MA, Andrianantenaina HB, Ariey F, Raveloson A, Domarle O, Randrianarivelojosia M: Prevalent pfmdr1 N86Y mutant Plasmodium falciparum in Madagascar despite absence of pfcrt mutant strains. Am J Trop Med Hyg. 2007, 76: 1079-1083.PubMed
- Randrianarivelojosia M, Sahondra-Harisoa JL, Raharimalala LA, Raveloson A, Mauclere P, Ariey F: A network RER rooted on in vitro readout assays of Plasmodium falciparum sensitivity to chloroquine in the Indian Ocean Region. Sante. 2003, 13: 95-100.PubMed
- Randrianarivelojosia M, Rakotonjanabelo LA, Mauclere P, Ratsimbasoa A, Raharimalala LA, Ariey F: National Network study to perpetuate the surveillance of Plasmodium falciparum sensitivity to antimalarials in Madagascar. Arch Inst Pasteur Madagascar. 2002, 68: 73-78.PubMed
- Randrianarivelojosia M, Harisoa JL, Rabarijaona LP, Raharimalala LA, Ranaivo L, Pietra V, Duchemin JB, Rakotomanana F, Robert V, Mauclere P, Ariey F: In vitro sensitivity of Plasmodium falciparum to amodiaquine compared with other major antimalarials in Madagascar. Parassitologia. 2002, 44: 141-147.PubMed
- Bodker R, Msangeni HA, Kisinza W, Lindsay SW: Relationship between the intensity of exposure to malaria parasites and infection in the Usambara Mountains, Tanzania. Am J Trop Med Hyg. 2006, 74: 716-723.PubMed
- Drakeley CJ, Carneiro I, Reyburn H, Malima R, Lusingu JP, Cox J, Theander TG, Nkya WM, Lemnge MM, Riley EM: Altitude-Dependent and -Independent Variations in Plasmodium falciparum Prevalence in Northeastern Tanzania. J Infect Dis. 2005, 191: 1589-1598. 10.1086/429669.View ArticlePubMed
- Marrama L, Rajaonarivelo E, Laventure S, Rabarison P: Anopheles funestus and rice agriculture in the Madagascar highlands. Sante. 1995, 5: 415-419.PubMed
- Craig MH, Kleinschmidt I, Nawn JB, Le Sueur D, Sharp BL: Exploring 30 years of malaria case data in KwaZulu-Natal, South Africa: part I. The impact of climatic factors. Trop Med Int Health. 2004, 9: 1247-1257. 10.1111/j.1365-3156.2004.01340.x.View ArticlePubMed
- Craig MH, Kleinschmidt I, Le Sueur D, Sharp BL: Exploring 30 years of malaria case data in KwaZulu-Natal, South Africa: part II. The impact of non-climatic factors. Trop Med Int Health. 2004, 9: 1258-1266. 10.1111/j.1365-3156.2004.01341.x.View ArticlePubMed
- Ijumba JN, Lindsay SW: Impact of irrigation on malaria in Africa: paddies paradox. Med Vet Entomol. 2001, 15: 1-11. 10.1046/j.1365-2915.2001.00279.x.View ArticlePubMed
- Mouchet J, Carnevale P, Coosemans M, Julvez J, Manguin S, Richard-Lenoble D, Sircoulon J: Biodiversité du paludisme dans le monde. John Libbey Eurotext edn. Paris. 2004
- Hay SI, Rogers DJ, Toomer JF, Snow RW: Annual Plasmodium falciparum entomological inoculation rates (EIR) across Africa: literature survey, Internet access and review. Trans R Soc Trop Med Hyg. 2000, 94: 113-127. 10.1016/S0035-9203(00)90246-3.PubMed CentralView ArticlePubMed
- Robert V, Le Goff G, Andrianaivolambo L, Randimby FM, Domarle O, Randrianarivelojosia M, Raharimanga V, Raveloson A, Ravaonjanahary C, Ariey F: Moderate transmission but high prevalence of malaria in Madagascar. Int J Parasitol. 2006, 36: 1273-1281. 10.1016/j.ijpara.2006.06.005.View ArticlePubMed
- Ralamboson D: Evolution du paludisme à Madagascar et lutte antipalustre. Annales de l'Université de Madagascar. 1964, 2: 123-133.
- Randriamanantena A, Randrianasolo L, Vonimpaisomihanta J, Tafangy P, Bayant Z, Randrianarivelojosia M: Efficacité therapeutique de l'amodiaquine à Madagascar. Cahiers Santé. 2007, 17: 75-78.PubMed
- Menard D, Andrianina NN, Ramiandrasoa Z, Randriamanantena A, Rasoarilalao N, Jahevitra M, Ratsimbasoa A, Tuseo L, Raveloson A: Randomized clinical trial of artemisinin versus non-artemisinin combination therapy for uncomplicated falciparum malaria in Madagascar. Malar J. 2007, 6: 65-10.1186/1475-2875-6-65.PubMed CentralView ArticlePubMed
- Ndiaye JL, Randrianarivelojosia M, Sagara I, Brasseur P, Ndiaye I, Faye B, Randrianasolo L, Ratsimbasoa A, Forlemu D, Moor VA, Traore A, Dicko Y, Dara N, Lameyre V, Diallo M, Djimde A, Same-Ekobo A, Gaye O: Randomized, multicentre assessment of the efficacy and safety of ASAQ – a fixed-dose artesunate-amodiaquine combination therapy in the treatment of uncomplicated Plasmodium falciparum malaria. Malar J. 2009, 8: 125-10.1186/1475-2875-8-125.PubMed CentralView ArticlePubMed
- Menard D, Ratsimbasoa A, Randrianarivelojosia M, Rabarijaona LP, Raharimalala L, Domarle O, Randrianasolo L, Randriamanantena A, Jahevitra M, Andriantsoanirina V, Rason MA, Raherinjafy R, Rakotomalala E, Tuseo L, Raveloson A: Assessment of the efficacy of antimalarial drugs recommended by the National Malaria Control Programme in Madagascar: Up-dated baseline data from randomized and multi-site clinical trials. Malar J. 2008, 7: 55-10.1186/1475-2875-7-55.PubMed CentralView ArticlePubMed
- Boisier P, Jambou R, Raharimalala L, Roux J: Relationship between parasite density and fever risk in a community exposed to a low level of malaria transmission in Madagascar highlands. Am J Trop Med Hyg. 2002, 67: 137-140.PubMed
- Randrianasolo L, Tafangy PB, Raharimalala LA, Ratsimbasoa AC, Randriamanantena A, Randrianarivelojosia M: [Rapid diagnostic test for malaria: Preliminary study in Madagascar in 2003.]. Sante. 2007, 17: 69-73.PubMed
- Rabezanahary HM, Rason MA, Carod JF, Randrianarivelojosia M: Bioquizz parasitologie. Revue Francophone des Laboratoires. 2009, 414: 67-68. 10.1016/S1773-035X(09)70092-9.View Article
- Mouchet J: [Origin of malaria epidemics on the plateaus of Madagascar and the mountains of east and south Africa]. Bull Soc Pathol Exot. 1998, 91: 64-66.PubMed
- O'Meara WP, Bejon P, Mwangi TW, Okiro EA, Peshu N, Snow RW, Newton CR, Marsh K: Effect of a fall in malaria transmission on morbidity and mortality in Kilifi, Kenya. Lancet. 2008, 372: 1555-1562. 10.1016/S0140-6736(08)61655-4.PubMed CentralView ArticlePubMed
- Ceesay SJ, Casals-Pascual C, Erskine J, Anya SE, Duah NO, Fulford AJ, Sesay SS, Abubakar I, Dunyo S, Sey O, Palmer A, Fofana M, Corrah T, Bojang KA, Whittle HC, Greenwood BM, Conway DJ: Changes in malaria indices between 1999 and 2007 in The Gambia: a retrospective analysis. Lancet. 2008, 372: 1545-1554. 10.1016/S0140-6736(08)61654-2.PubMed CentralView ArticlePubMed
- Steketee RW, Sipilanyambe N, Chimumbwa J, Banda JJ, Mohamed A, Miller J, Basu S, Miti SK, Campbell CC: National malaria control and scaling up for impact: the Zambia experience through 2006. Am J Trop Med Hyg. 2008, 79: 45-52.PubMed
- Okiro EA, Al-Taiar A, Reyburn H, Idro R, Berkley JA, Snow RW: Age patterns of severe paediatric malaria and their relationship to Plasmodium falciparum transmission intensity. Malar J. 2009, 8: 4-10.1186/1475-2875-8-4.PubMed CentralView ArticlePubMed
- Ratsimbasoa A, Randrianarivelojosia M, Millet P, Soares JL, Rabarijaona L, Rakotoson B, Malvy D, Menard D: Use of pre-packaged chloroquine for the home management of presumed malaria in Malagasy children. Malar J. 2006, 5: 79-10.1186/1475-2875-5-79.PubMed CentralView ArticlePubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.