Volume 9 Supplement 2

Parasite to Prevention: Advances in the understanding of malaria

Open Access

In vitro and in vivo antiplasmodial activity and cytotoxicity of extracts from Vernonia amygdalina Del. Leaves

  • Ehimwenma S Omoregie1,
  • Anirban Pal2,
  • Mahendra P Darokar3,
  • Debabrata Chanda2 and
  • Brijesh Sisodia3
Malaria Journal20109(Suppl 2):P30

DOI: 10.1186/1475-2875-9-S2-P30

Published: 20 October 2010

Background

This study evaluated the in vitro and in vivo antiplasmodial activity of extracts from V. amygdalina leaves. The plant was selected based on local claims on its efficacy as part of the treatment regimen in malarial infection in the south - western region of Nigeria.

Materials and methods

Extracts of the plant (ethanolic, aqueous, and hydroethanolic (50:50) extracts) were prepared using standard procedures. Chemical profile of the extracts was performed through high performance thin layer chromatography (HPTLC) for quality control. The extracts were evaluated in vitro for antiplasmodial activity using a 3D7 chloroquine sensitive clone of NF-54 isolate of Plasmodium falciparum. The parasite growth inhibition was estimated based on the 48 hours microassay technique [1]. Cytotoxicity of these extracts was evaluated in vitro against non-cancerous vero cell lines (C-1008 kidney fibroblasts from African green monkey) by the neural red uptake method [2]. The in vivo antiplasmodial activity of the most active extract(s) was assessed based on the standard four days suppressive test on P. berghei (ANKA) infected male mice (six weeks old) of the Swiss strain [3].

Results

Results from the in vitro study showed that the ethanolic extract of the plant leaves had the highest (p<0.05) antiplasmodial activity (IC50 = 9.83 ug/ml) and cytotoxicity (IC50 = 60.33) with moderate selectivity index of 6. 14 when compared with the other extracts. The ethanolic extract was also significantly active in vivo against P. berghei in a dose-dependent manner with maximum activity observed at 1000 mg/kg (% inhibition of 82.3 %). There was also a dose-dependent significant decrease (p<0.05) in some oxidative stress indices especially nitric oxide and malonaldehyde levels. The pro-inflammatory cytokines (TNF-α and IFN-γ) levels were also considerably low relative to control values.

Conclusions

The results suggest that V. amygdalina possess moderate antiplasmodial activity both in vitro and in vivo. The immunomodulatory and antioxidant activities of this extract may be responsible for its antiplasmodial property. The study therefore confirms local claims on the use of the plant leaves as part of the treatment regimen in malarial infection.

Declarations

Acknowledgements

We wish to acknowledge The Academy of Sciences for the developing World (TWAS) and the Council for Scientific and Industrial Research (CSIR) - (TWAS-CSIR) for funding this research.

Authors’ Affiliations

(1)
Department of Biochemistry, Faculty of Life Sciences, University of Benin
(2)
In vivo Animal Testing Facility, Central Institute of Medicinal and Aromatic Plants (CIMAP)
(3)
In vitro Cell Culture Laboratory, Genetic Resources and Biotechnology Department, Central Institute of Medicinal and Aromatic Plants (CIMAP)

References

  1. Trager W, Jensen JB: Human malaria parasites in continuous culture. Science. 1976, 193: 673-675. 10.1126/science.781840.View ArticlePubMedGoogle Scholar
  2. Fotakis G, Timbrell JA: In vitro cytotoxicity assays-comparison of LDH, neural red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett. 2006, 160: 171-177. 10.1016/j.toxlet.2005.07.001.View ArticlePubMedGoogle Scholar
  3. Peters W, Robinson BL: The chemotherapy of rodent malaria XLVII: studies on pyronaridine and other Man-nich base antimalarials. Ann Trop Med Parasitol. 1992, 86: 455-465.PubMedGoogle Scholar

Copyright

© Omoregie et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Advertisement