Absence of in vivo selection for K13 mutations after artemether–lumefantrine treatment in Uganda
- Betty Balikagala1,
- Toshihiro Mita2Email author,
- Mie Ikeda2,
- Miki Sakurai3,
- Shouki Yatsushiro4,
- Nobuyuki Takahashi3,
- Shin-Ichiro Tachibana2,
- Mary Auma5,
- Edward H. Ntege1,
- Daisuke Ito1,
- Eizo Takashima1,
- Nirianne Marie Q. Palacpac6,
- Thomas G. Egwang7,
- Joseph Okello Onen^8,
- Masatoshi Kataoka4,
- Eisaku Kimura6,
- Toshihiro Horii6 and
- Takafumi Tsuboi1Email author
© The Author(s) 2017
Received: 3 November 2016
Accepted: 20 December 2016
Published: 9 January 2017
Individual drug treatment may select resistant parasites in the human body, a process termed in vivo selection. Some single nucleotide polymorphisms in Plasmodium falciparum chloroquine-resistance transporter (pfcrt) and multidrug resistance gene 1 (pfmdr1) genes have been reportedly selected after artemether–lumefantrine treatment. However, there is a paucity of data regarding in vivo selection of P. falciparum Kelch propeller domain (pfkelch13) polymorphisms, responsible for artemisinin-resistance in Asia, and six putative background mutations for artemisinin resistance; D193Y in ferredoxin, T484I in multiple resistance protein 2, V127M in apicoplast ribosomal protein S10, I356T in pfcrt, V1157L in protein phosphatase and C1484F in phosphoinositide-binding protein.
Artemether–lumefantrine efficacy study with a follow-up period of 28 days was conducted in northern Uganda in 2014. The above-mentioned genotypes were comparatively analysed before drug administration and on days; 3, 7, and 28 days after treatment.
In 61 individuals with successful follow-up, artemether–lumefantrine treatment regimen was very effective with PCR adjusted efficacy of 95.2%. Among 146 isolates obtained before treatment, wild-type alleles were observed in 98.6% of isolates in pfkelch13 and in all isolates in the six putative background genes except I356T in pfcrt, which had 2.4% of isolates as mixed infections. In vivo selection study revealed that all isolates detected in the follow-up period harboured wild type alleles in pfkelch13 and the six background genes.
Mutations in pfkelch13 and the six background genes may not play an important role in the in vivo selection after artemether–lumefantrine treatment in Uganda. Different mechanisms might rather be associated with the existence of parasites after treatment.
KeywordsIn vivo selection pfkelch13 Artemether–lumefantrine Drug resistance Polymorphisms Plasmodium falciparum
Since the mid-2000s, artemisinin-based combination therapy (ACT) has been deployed as first-line treatment for uncomplicated Plasmodium falciparum malaria in nearly all malaria endemic countries . The wide-scale deployment has been regarded as one of the central causes of the recent decline in malaria related morbidity and mortality rates . However, since the first report of artemisinin-resistant P. falciparum malaria in Western Cambodia , geographical areas of artemisinin resistance have steadily spread into the Greater Mekong sub-region [4, 5]. In Africa, although previous clinical trials have demonstrated rapid parasite-clearance after ACT treatment , there is a global concern that artemisinin resistance may invade this region following the path previously observed in chloroquine and sulfadoxine/pyrimethamine resistance [7–10].
In 2014, pfkelch13 (PF3D7_1343700) was identified as a useful molecular marker for tracking the emergence and spread of artemisinin resistant P. falciparum. Pfkelch13 encodes a 726 amino acid protein with a broad-complex, tramtrack, bric-a-brac/poxvirus and zincfinger (BTB/POZ) domain and a C-terminal 6-blade propeller domain . Some mutations in these two domains are associated with delayed parasite-clearance time following artemisinin treatment in Southeast Asia [4, 11]. In addition, a recent genome-wide association study has identified several single nucleotide polymorphisms (SNPs) that are assumed to be background genetic changes for artemisinin resistance, these include; D193Y in ferredoxin (fd), T484I in multidrug resistance protein 2+ (mdr2), V127M in the apicoplast ribosomal protein S10 (arps10), I356T in chloroquine-resistance transporter (crt), V1157L in protein phosphatase (pph) and C1484F in phosphoinositide-binding protein (pibp) . In Africa, polymorphisms in these genes have been occasionally observed, but mostly different from those reported in Southeast Asia [13, 14]. Therefore, it remains unclear whether the existence of these mutations in Africa is a consequence of selection induced by anti-malarial usage.
During the period of antimalarial treatment, less susceptible parasites can be selected in the human body, a process termed in vivo selection. This is because the treatment would create drug concentration circumstances that are sufficient to kill susceptible, but not less susceptible parasites. Previous investigations revealed that artemether–lumefantrine (AL) treatment selected for parasites harbouring alleles with K76 in pfcrt and N86, 184F and D1246 in pfmdr1 [15–17]. However, the possibility of similar in vivo selection has not been fully investigated in pfkelch13  and the putative background genes.
In Uganda, AL was adopted as a first line treatment for uncomplicated malaria in 2004, but actual implementation was in 2006. So far, marked clinical efficacy has been reported to this regimen; 0–0.5% of cases with residual parasites by day 3 [19–28] and 1.0–6.0% of recrudescence [20, 21, 24, 26]. All these values were determined microscopically. Molecular assessments using high-sensitive PCR are able to detect sub-microscopic infection of parasites during follow-up after anti-malarial treatment, some of which might possess resistant phenotypes . In this study, AL clinical efficacy in Gulu, Northern Uganda was assessed. Recruited individuals were followed up for 28 days after treatment and presence of parasites was determined by polymerase chain reaction (PCR) to detect sub-microscopic infections. Genotyping of pfkelch13 polymorphisms, and putative background SNPs for artemisinin resistance in P. falciparum recurrent infections was done to evaluate whether AL treatment selected for polymorphisms in a region where it has been used for a long time.
Study site and patients
The study was conducted at the peak of malaria transmission between May–July and October–November 2014 at St Mary’s Hospital Lacor in Gulu district, Northern Uganda. Malaria transmission in the study region is perennial with an estimated prevalence of >60%, and entomological inoculation rate (EIR) of 100 or more infective mosquito bites per person per year . Anopheles funestus is the major mosquito vector and a few infections are due to Anopheles gambiae . Malaria control measures in the region include, indoor residual spraying (IRS), long-lasting insecticide-treated nets (LLINs), malaria case management with ACT and intermittent preventive treatment during pregnancy (IPTp). In particular, IRS has been scaled-up since 2009 to cover 10 high-malaria burden districts (Apac, Kole, Gulu, Amuru, Nwoya, Pader, Agago, Kitgum, Oyam and Lamwo) in the mid-northern region . Symptomatic individuals with P. falciparum positive results by rapid diagnostic test (RDT) were referred to the study physicians. The criteria for recruitment are shown in Additional file 1. Individuals who had received anti-malarial treatment within two weeks prior to enrollment were excluded from AL efficacy study but recruited in the in vivo selection study.
Ethics, consent and permissions
Before enrollment, written informed consent was obtained from the participants’ parents or guardians, and children aged ≥7 years were assented. The study was reviewed and approved by Lacor Hospital Institutional Research and Ethics Committee (LHIREC) (Study protocol number LHIREC 008/05/2013 and 021/09/13) and regulatory approval was obtained from the Uganda National Council for Science and Technology (UNCST) (HS 1395).
Artemether–lumefantrine treatment and follow-up assessment
For the efficacy study, AL (Coartem®, Novartis 20 mg artemether/120 mg lumefantrine tablets) was orally administered twice daily for 3 days and follow-up assessments were performed on days 1, 2, 3, 7, and 28 after initial drug treatment. Dosage of oral Coartem® was adjusted according to the participant’s body weight: one (5–14 kg), two (15–24 kg), or three (25–34 kg) tablets. The drug was given as directly observed treatment (DOTS) for all patients by study nurses and physicians. After each treatment, patients were carefully observed for 30 min, and the same dose was re-administered if vomiting occurred. Rescue treatment regimen (dihydroartemisinin–piperaquine) was administered daily for 3 days to any individuals who failed on the initial AL therapy. If the recruited patients developed severe malaria during follow-up, they were referred to the hospital for parenteral artesunate.
At enrollment, venous blood samples (1 mL) were obtained from the cubital vein before initial treatment except for children <2 years where finger prick sampling was performed. A finger-prick blood sample of 100 μL was obtained at each follow-up visit. Blood was spotted on chromatography filter paper (ET31CHR; Whatman Limited, Kent, UK). Haemoglobin (Hb) concentration was measured using a portable spectrophotometer Hemocue Hb 201 (HemoCue, Ängelholm, Sweden) on days 0 and 28 or on the day of late clinical failure. Anaemic patients with Hb level <10.0 g/dL were treated with Ferrous sulphate tablets for 14 days. Plasma concentrations of artemether and lumefantrine were not measured. Treatment outcomes were classified according to WHO guidelines for areas of intense malaria transmission as: adequate clinical and parasitological response (ACPR), early treatment failure (ETF), late clinical failure (LCF) and late parasitological failure (LPF) .
Microscopic and molecular diagnosis of malaria parasites
Thick and thin blood smears were stained with 2% Giemsa for 30 min. The number of parasites was counted per 200 white blood cells (WBCs), assuming 8000 WBC/µL. Parasite density was calculated by averaging independent counts made by two microscopists. Discordant results (difference in parasite density of >50%) were re-examined by a third microscopist and, parasite density calculated by averaging two closest counts. Slides were considered negative if after examination of thick smears, no parasite was detected in 100 high power fields. Parasite DNA was extracted from a quarter of dried blood spot (25 µL) using a QIAamp DNA Kit (Qiagen, Hilden, Germany) . In all first visit and follow-up cases, P. falciparum infections were assessed by species-specific PCR as previously described . Genotyping of merozoite surface protein 1 (msp1), merozoite surface protein 2 (msp-2) and glutamate rich protein (glurp), was also performed to differentiate between recrudescence and new infections on day 0 and the day of positive infection [36, 37]. Nested PCR products were analysed by electrophoresis using 2% agarose for msp-1 and msp-2 and 1.5% agarose for glurp. Patient samples were run side by side. Gel images were digitised and molecular weights determined using imagej software , an open platform for scientific image analysis. Densitometric curves were generated for each gel lane, and dominant bands in each lane were assigned molecular weights. Using reference strains, alleles were considered the same if molecular weights were within 10 bp for msp 2 and 20 bp for glurp.
Genotyping of drug-resistant genes
Polymorphisms in pfcrt (K76T) and pfmdr1 (N86Y, Y184F, S1034C, N1042D and D1246Y) were determined as reported [39, 40]. K13-propeller domain was amplified by nested PCR, covering almost all the six propeller domain sequences, as described . The sequences were aligned using MUSCLE in MEGA software, version 6.06  with P. falciparum 3D7 full-length sequence of K13-propeller domain (PF3D7_1343700) from PlasmoDB  as reference.
Six background mutations for artemisinin resistance (D193Y in fd, T484I in mdr2, V127M in arps10, I356T in crt, V1157L in pph and C1484F in pibp) were amplified by multiplex PCR using gene specific primers (Additional file 2). In brief, 10 μL reaction mixture consisted of 1 μL of DNA template, 0.5 μM of 6-primer sets, and PrimeSTAR Max DNA Polymerase (Takara Bio Inc., Otsu, Japan). Cycling conditions were: denaturation at 98 °C for 10 s, followed by 40 cycles of amplification (98 °C for 10 s, 60 °C for 10 s, and 72 °C for 90 s), with a final elongation period of 90 s at 72 °C. ExoSAP-IT Kit was used for the purification of PCR products. SNP typing was performed on amplified products with 5 μL of reaction mixture consisting of 2.5 μL of Premix Ex Taq (Probe qPCR) (Takara Bio Inc.), 0.2 μM of each primer, 0.1 μM of LNA probe set, 0.05 μL of ROX Reference Dye II, and 0.5 μL of template DNA using the 7500 Real-Time PCR System (Applied Biosystems). Primers and probes for SNP assay are also shown (Additional file 2). The probes for detecting wild type and mutant SNPs were labeled with HEX and 6-FAM (6-carboxyfluorescein) as reporters at 5′ end, respectively. All probes contained Iowa Black® FQ (IBFQ) as a dark quencher at 3′ end.
To evaluate performance of the SNP assay system in all 6 genes, two P. falciparum strains, wild type (3D7) and a mutant-type (a strain from Thailand identified during preliminary experiments) were used as positive controls. Nucleotide sequence data are available in the GenBank™, EMBL, and DDBJ databases under the accession numbers: LC193525–LC193693.
Kaplan–Meier product limit formula was used for the estimation of day 28-PCR-adjusted cure rates, which was the primary efficacy endpoint. The uncorrected and PCR-corrected Kaplan–Meier cumulative treatment success rates up to day 28 were calculated for all participants. Patients were censored when either lost to follow-up or withdrawn from the study. Either Fisher’s exact test or Pearson’s Chi square test (χ2) test were used for comparison of categorical variables, and Wilcoxon rank sum test was used for the continuous variables. P values <0.05 were considered statistically significant. R statistical software (version 3.2.0; R Foundation for Statistical Computing) was used for all the statistical analyses.
Characteristics of 61 individuals in artemether–lumefantrine efficacy study at enrollment
<5 years (n = 49)
≥5 years (n = 12)
Gender ratio (male/female)
2.7 ± 13.0
5.9 ± 18.8
38.4 ± 1.3
38.5 ± 1.0
Geometric mean parasitaemia (No. of parasites/μL)
10,429.8 ± 10.4
17,746.4 ± 12.4
10.0 ± 1.9
10.8 ± 2.5
Gametocytes present (n)
Artemether–lumefantrine treatment outcomes
Response to artemether–lumefantrine treatment
Total (n = 61)
<5 years (n = 49)
≥5 years (n = 12)
Fever (≥37.5 °C) persistence [n (%)]
Microscopy [n (%)]
PCR [n (%)]
Gametocyte persistence [n (%)]
28-day WHO treatment outcome [n (%)]
Early treatment failure
Late clinical failure
Late parasitological failure
Adequate clinical and parasitological response
Cure rate [n (%)]
Similar to the excellent early treatment response, cumulative-efficacy of AL was high with ACPR ratio of 95.2% (Table 2). Only three individuals showed LCF on day 28 and were later confirmed as reinfection by msp1, msp2 and glurp genotyping (Additional file 4). These children were all male, aged; 11 months, 3 and 6 years with enrollment temperature of 36.8, 39.2 and 39.1 °C and initial parasitaemia of 1400 μL (0.03%), 99,200 μL (2.2%) and 96,080 μL (2.1%), respectively. Although all were parasite negative by day 2, they had malaria recurrence on day 28 with parasitaemia of 1840 μL (0.04%), 8460 μL (0.2%) and 32,120 μL (0.7%), respectively. The children were successfully treated with the rescue regimen. Gametocytes were observed in five individuals (8.2%) at enrolment, and in two cases, persisted until day 7 even after AL treatment.
Drug-resistance related alleles at enrollment
Prevalence of amino acid substitutions in the putative drug-resistance related genes in Plasmodium falciparum isolates collected before artemether–lumefantrine treatment
Amino acid position
Wild type (n, %)
Mutant (n, %)
Mixed (n, %)
2 (1.4) A578S
In vivo selection of drug-resistance related alleles after artemether–lumefantrine treatment
The present study revealed that both early and late responses to AL were still excellent in this study region of northern Uganda even after 8 years of its actual implementation as first-line treatment. Only one individual (1.6%) showed microscopically detectable parasites by day 3. Overall, similar excellent early response to AL treatment has been reported in other regions in Uganda [19–28]. According to WHO criteria  an endemic region showing ≥10% cases with detectable parasites on day 3 after ACT treatment is regarded as an area with suspected artemisinin resistance. The Worldwide Antimalarial Resistance Network (WWARN) proposes a more sensitive benchmark of 5% threshold for sub-Saharan Africa because of higher levels of herd immunity to malaria in the region . In all cases, however, the prevalence of day 3 parasite positive individuals in the present study was less than the benchmarks for artemisinin-resistance.
PCR-confirmed parasite positivity after AL treatment was much higher than microscopically confirmed positivity; 91.8% on day 1, 54.1% on day 2, and 23% on day 3. These prevalences were similar to two previous studies that molecularly assessed parasite positivity in Kenya and Tanzania [45, 46]. Beshir et al.  reported that PCR-confirmed parasite positivity on day 3 would be a good predictor for malaria recurrence. In this study, however, recurrence frequencies did not differ much between the PCR-confirmed parasite positive group (7.7%) and the PCR-confirmed parasite negative group (4.2%) on day 3.
Mechanisms of artemisinin resistance have been gradually uncovered albeit the overall picture has not been clarified. Enhanced stress response including activation of unfolded protein response and the PI3K/Pi3P/AKT pathway is thought to be the main mechanism for parasite survival in the presence of artemisinin [47, 48]. Pfkelch13 has been elucidated to be involved in these processes [49, 50]. In the present analysis, however, nearly all parasites harboured wild-type alleles in pfkelch13 at enrollment. The only mutation observed in Pfkelch13 was A578S, which has been widely distributed in Africa [13, 14]. Computational modelling reported that A578S could potentially disrupt the normal function of the Pfkelch13 protein . However, only one study has described a close link between A578S and prolonged parasite clearance after artemisinin treatment  and others reported no association [14, 18, 53]. Very recently, it has been described that introduction of A578S mutation into Dd2 did not change the in vitro artemisinin susceptibility determined by ring-stage survival assay . This observation partially supports the idea that A578S is not an artemisinin resistance related mutation. However, acquirement of artemisinin resistance would be a consequence of multiple genetic changes. As observed in this study and others , genetic background was different between African and Southeast Asian parasites. Since Dd2 clone is derived from Indochina, similar transfection studies using African parasite in addition to further in vivo efficacy study and population genetic assessment would be required to determine the potential role of A578S mutation.
In vivo selection analysis revealed that pfkelch13 mutation was not observed in the parasite positive samples on day 3, 7 and 28, consistent with the recent observations . Also, no selection of putative six non-synonymous polymorphisms was observed, suggesting that these genetic changes would not be responsible for parasite persistence in the present study. In contrast, Pfcrt K76 and Pfmdr1 N86/D1246 were observed in all recurrent parasites. Prevalence of Pfmdr1 Y184F (33.3%) in the recurrent patients was higher than baseline (14%), although not statistically significant. These observations support the potential selection of Pfcrt K76 and Pfmdr1 N86/Y184F/D1246 after AL treatment [16, 17]. In vivo selection of these mutations would increase these allele prevalences in the parasite population. In fact, the analysis herein revealed much higher allele frequencies than previously reported ; 68 versus 0% in the Pfcrt K76, 98.0 versus 9.5% in Pfmdr1 N86 and 93.7 versus 16.9% in Pfmdr1 D1246.
One patient showed day 3 parasite positivity in the present study. Multiple factors are associated with delayed parasite clearance after AL treatment . Quality-assured artemether–lumefantrine, Coartem®, was used and all treatment were administered at the study hospital using DOTS, suggesting that drug factors were very unlikely to have major contribution to the early treatment failure. High initial parasitaemia is considered to be the most contributing factor to parasite positivity on day 3 . However, the baseline parasitaemia of this patient was only 0.85%, precluding this possibility. This individual suffered from sickle cell anaemia with a haemoglobin level of 7.8 g/dL. Splenic function to filter parasitized red blood cells is impaired in sickle cell anaemia patients . Previous meta-analysis also reported that severe anaemia was associated with slow parasite clearance and indicative of a poor immune response [44, 56]. Additionally, infected parasites carried Pfmdr1 allele combination (N86 and D1246) that was reported to be associated with sub-microscopic residual parasites on day 3 . Taken together, the host factors in addition to parasite innate resistant potential might have functioned in the observed impaired early response.
Role of the human immunodeficiency virus (HIV)-infection to malaria treatment efficacy with ACT remains to be elusive. Some studies showed a close association between HIV infection and lower ACT treatment outcome , but others negated this association [24, 59]. In this study, HIV infection was not determined, mainly because nearly all patients showed excellent ACT efficacy, and thus, this would not be suitable for the association study. However, future studies are expected to clarify the potential role of HIV infection to ACT efficacy.
Unlike previous studies with malaria recurrence rates of 35–60% after artemether–lumefantrine treatment in Uganda [20–24, 26], much lower rates (4.9%) were observed in the study area. This can be explained by considerably fewer cases of reinfection in this study. Moreover, this is most likely due to considerable decrease in malaria prevalence in the study site after the scaled-up indoor residual spraying program in the mid-northern region .
This study demonstrated that AL treatment remains of high efficacy for the treatment of P. falciparum malaria after 8 years of use in a region of high malaria transmission in Uganda. No clear evidence was obtained for the selection of mutant alleles in pfkelch13 and the six-background genes, all of which have been reported to be associated with artemisinin resistance in Southeast Asia. Close monitoring of AL efficacy is necessary as we seek to understand the influence of anti-malarial treatments and parasite persistence, malaria transmission setting and different genetic background of parasites and host interactions in the mutational process. This work was done where very low early treatment failure and recurrence as well as scarcity of mutant alleles in pfkelch13 and the six-background genes were observed; if the circumstances change, repeated in vivo selection analysis is recommended.
BB, TM, TH and TT conceived and designed the study. BB, TM, MI, MS, SY, MA, EHN, NMQP, TGE, JOO, MK, EK participated in field data collection. BB, NT, ST, DI, ET performed experiments. BB, TM, TH and TT analysed and interpreted the data. BB, TM, EHN, NMQP, TH and TT wrote and revised the paper for important intellectual content. All authors read and approved the final manuscript.
We thank all research teams from Gulu University; St. Mary’s Hospital Lacor; Department of Molecular and Cellular Parasitology, Juntendo University; Malaria Research Division, Proteo-Science Center, Ehime University and the Research Institute for Microbial Diseases, Osaka University for their technical assistance in obtaining the field isolates. We thank the parents and guardians who kindly provided consent and the study participants for their overwhelming cooperation.
The authors declare that they have no competing interests.
Availability of data and materials
The primary datasets used and analysed during the current study are available from the corresponding author on reasonable request.
Consent for publication
Prior to participation, all study subjects consented to the publication of study results in the medical literature in an anonymised manner.
This research was supported in part by Strategic Promotion of International Cooperation to Accelerate Innovation in Africa by MEXT, JSPS KAKENHI, Japan (JP26240101, JP26253026, JP26670202, JP15H05276, JP16K15266), Global Health Innovative Technology Fund (G2013-105), and the Research on Development of New Medical Devices from Japan Agency for Medical Research and Development, AMED.
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