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  1. Methodology

    Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene

    Molecular tools for detecting malaria-infected mosquitoes with improved practicality, sensitivity and specificity, and high-throughput are required. A common PCR technique used to detect mosquitoes infected with

    Diego F. Echeverry, Nicholas A. Deason, Victoria Makuru, Jenna Davidson, Honglin Xiao, Julie Niedbalski, Xiaoyu Yu, Jennifer C. Stevenson, Hugo Bugoro, Allan Aparaimo, Hedrick Reuben, Robert Cooper, Thomas R. Burkot, Tanya L. Russell, Frank H. Collins and Neil F. Lobo

    Malaria Journal 2017 16:230

    Published on: 31 May 2017

  2. Research

    Evaluation of the knowledge and attitude of pharmacists about the national malaria control policy in southern Benin

    The national strategy against malaria in an endemic country should involve all the health stakeholders. In Benin, the private sector is rarely present in the activities of the National Malaria Control Programm...

    Habib Ganfon, Giraud Ekanmian, Louis Amoussou, Emilie Daniel-Garcia and Aurel Constant Allabi

    Malaria Journal 2017 16:231

    Published on: 31 May 2017

  3. Research

    A metabolomic analytical approach permits identification of urinary biomarkers for Plasmodium falciparum infection: a case–control study

    Currently available diagnostic techniques of Plasmodium falciparum infection are not optimal for non-invasive, population-based screening for malaria. It was hypothesized that a mass spectrometry-based metabolomi...

    Salah Abdelrazig, Catharine A. Ortori, Gail Davey, Wakgari Deressa, Dhaba Mulleta, David A. Barrett, Alemayehu Amberbir and Andrew W. Fogarty

    Malaria Journal 2017 16:229

    Published on: 30 May 2017

  4. Research

    Rapid reduction of malaria following introduction of vector control interventions in Tororo District, Uganda: a descriptive study

    In 2012, Tororo District had the highest malaria burden in Uganda with community Plasmodium prevalence of 48%. To control malaria in the district, the Ministry of Health introduced universal distribution of long ...

    David W. Oguttu, Joseph K. B. Matovu, David C. Okumu, Alex R. Ario, Allen E. Okullo, Jimmy Opigo and Victoria Nankabirwa

    Malaria Journal 2017 16:227

    Published on: 30 May 2017

  5. Research

    Molecular and immunological analyses of confirmed Plasmodium vivax relapse episodes

    Relapse infections resulting from the activation hypnozoites produced by Plasmodium vivax and Plasmodium ovale represent an important obstacle to the successful control of these species. A single licensed drug, p...

    Sarunya Maneerattanasak, Panita Gosi, Srivicha Krudsood, Pattamawan Chimma, Jarinee Tongshoob, Yuvadee Mahakunkijcharoen, Chonlaphat Sukasem, Mallika Imwong, Georges Snounou and Srisin Khusmith

    Malaria Journal 2017 16:228

    Published on: 30 May 2017

  6. Case Study

    Elimination of Plasmodium falciparum malaria in Tajikistan

    Malaria was eliminated in Tajikistan by the beginning of the 1960s. However, sporadic introduced cases of malaria occurred subsequently probably as a result of transmission from infected mosquito Anopheles fly...

    Anatoly V. Kondrashin, Azizullo S. Sharipov, Dilshod S. Kadamov, Saifuddin S. Karimov, Elkhan Gasimov, Alla M. Baranova, Lola F. Morozova, Ekaterina V. Stepanova, Natalia A. Turbabina, Maria S. Maksimova and Evgeny N. Morozov

    Malaria Journal 2017 16:226

    Published on: 30 May 2017

  7. Research

    Insecticide-treated nets provide protection against malaria to children in an area of insecticide resistance in Southern Benin

    Malaria control is heavily reliant on insecticides, especially pyrethroids. Resistance of mosquitoes to insecticides may threaten the effectiveness of insecticide-based vector control and lead to a resurgence ...

    John Bradley, Aurore Ogouyèmi-Hounto, Sylvie Cornélie, Jacob Fassinou, Yolande Sissinto Savi de Tove, Adicath Adéola Adéothy, Filémon T. Tokponnon, Patrick Makoutode, Alioun Adechoubou, Thibaut Legba, Telesphore Houansou, Dorothée Kinde-Gazard, Martin C. Akogbeto, Achille Massougbodji, Tessa Bellamy Knox, Martin Donnelly…

    Malaria Journal 2017 16:225

    Published on: 26 May 2017

  8. Research

    Effect of IPTp on Plasmodium falciparum antibody levels among pregnant women and their babies in a sub-urban coastal area in Ghana

    Women exposed to Plasmodium infection develop antibodies and become semi-immune. This immunity is suppressed during pregnancy making both the pregnant woman and the foetus vulnerable to the adverse effects of mal...

    Judith K. Stephens, Eric Kyei-Baafour, Emmanuel K. Dickson, Jones K. Ofori, Michael F. Ofori, Mark L. Wilson, Isabella A. Quakyi and Bartholomew D. Akanmori

    Malaria Journal 2017 16:224

    Published on: 26 May 2017

  9. Research

    Assessment of coverage of preventive treatment and insecticide-treated mosquito nets in pregnant women attending antenatal care services in 11 districts in Mozambique in 2011: the critical role of supply chain

    Malaria during pregnancy is associated with poor maternal and pregnancy outcome and the World Health Organization recommends the administration of intermittent preventive treatment in pregnancy (IPTp) with sul...

    Cristolde Salomão, Jahit Sacarlal and Eduardo Samo Gudo

    Malaria Journal 2017 16:223

    Published on: 25 May 2017

  10. Research

    Evaluation of a laboratory quality assurance pilot programme for malaria diagnostics in low-transmission areas of Kenya, 2013

    One objective of the Kenya National Malaria Strategy 2009–2017 is scaling access to prompt diagnosis and effective treatment. In 2013, a quality assurance (QA) pilot was implemented to improve accuracy of mala...

    Elizabeth Wanja, Rachel Achilla, Peter Obare, Rose Adeny, Caroline Moseti, Victor Otieno, Collins Morang’a, Ephantus Murigi, John Nyamuni, Derek R. Monthei, Bernhards Ogutu and Ann M. Buff

    Malaria Journal 2017 16:221

    Published on: 25 May 2017

  11. Research

    Effect of climatic variability on malaria trends in Baringo County, Kenya

    Malaria transmission in arid and semi-arid regions of Kenya such as Baringo County, is seasonal and often influenced by climatic factors. Unravelling the relationship between climate variables and malaria tran...

    Edwin K. Kipruto, Alfred O. Ochieng, Douglas N. Anyona, Macrae Mbalanya, Edna N. Mutua, Daniel Onguru, Isaac K. Nyamongo and Benson B. A. Estambale

    Malaria Journal 2017 16:220

    Published on: 25 May 2017

  12. Research

    Within-host selection of drug resistance in a mouse model of repeated interrupted treatment of Plasmodium yoelii infection

    To study within-host selection of resistant parasites, an important factor in the development of resistance to anti-malarial drugs, a mouse model of repeated interrupted malaria treatment (RIT) has been develo...

    Suci Nuralitha, Josephine E. Siregar, Din Syafruddin, Andy I. M. Hoepelman and Sangkot Marzuki

    Malaria Journal 2017 16:216

    Published on: 23 May 2017

  13. Case Study

    Intermittent preventive treatment for malaria among children in a refugee camp in Northern Uganda: lessons learned

    Northern Uganda hosts a large population of refugees from South Sudan, and malaria is one of the major health problems in the area. In 2015, intermittent preventive treatment for malaria (IPTc) was implemented...

    Matthew E. Coldiron, Estrella Lasry, Malika Bouhenia, Debashish Das, Peter Okui, Dan Nyehangane, Juliet Mwanga, Celine Langendorf, Greg Elder, Léon Salumu and Rebecca F. Grais

    Malaria Journal 2017 16:218

    Published on: 23 May 2017

  14. Research

    Safety and tolerability of adjunctive rosiglitazone treatment for children with uncomplicated malaria

    Despite the widespread use and availability of rapidly acting anti-malarials, the fatality rate of severe malaria in sub-Saharan Africa remains high. Adjunctive therapies that target the host response to malar...

    Rosauro Varo, Valerie M. Crowley, Antonio Sitoe, Lola Madrid, Lena Serghides, Rubao Bila, Helio Mucavele, Alfredo Mayor, Quique Bassat and Kevin C. Kain

    Malaria Journal 2017 16:215

    Published on: 23 May 2017

  15. Research

    Molecular markers of anti-malarial drug resistance in Central, West and East African children with severe malaria

    The Plasmodium falciparum multidrug resistance 1 (PfMDR1), P. falciparum Ca2+-ATPase (PfATP6) and Kelch-13 propeller domain (PfK13) loci are molecular markers of parasite susceptibility to anti-malarial drugs. Th...

    Christian N. Nguetse, Ayola Akim Adegnika, Tsiri Agbenyega, Bernhards R. Ogutu, Sanjeev Krishna, Peter G. Kremsner and Thirumalaisamy P. Velavan

    Malaria Journal 2017 16:217

    Published on: 23 May 2017

  16. Research

    Glucose-6-phosphate dehydrogenase deficiency in people living in malaria endemic districts of Nepal

    Glucose-6-phosphate dehydrogenase (G6PD) is a rate limiting enzyme of the pentose phosphate pathway and is closely associated with the haemolytic disorders among patients receiving anti-malarial drugs, such as...

    Prakash Ghimire, Nihal Singh, Leonard Ortega, Komal Raj Rijal, Bipin Adhikari, Garib Das Thakur and Baburam Marasini

    Malaria Journal 2017 16:214

    Published on: 23 May 2017

  17. Research

    Naturally acquired humoral and cellular immune responses to Plasmodium vivax merozoite surface protein 8 in patients with P. vivax infection

    Thirty-one glycosylphosphatidylinositol (GPI)-anchored proteins of Plasmodium vivax, merozoite surface protein 1 (MSP1), MSP1 paralogue, MSP4, MSP5, MSP8, and MSP10 have been reported from homologs of Plasmodium ...

    Yang Cheng, Bo Wang, Siriruk Changrob, Jin-Hee Han, Jetsumon Sattabongkot, Kwon-Soo Ha, Patchanee Chootong, Feng Lu, Jun Cao, Myat Htut Nyunt, Won Sun Park, Seok-Ho Hong, Chae Seung Lim, Takafumi Tsuboi and Eun-Taek Han

    Malaria Journal 2017 16:211

    Published on: 22 May 2017

  18. Research

    Improving local health workers’ knowledge of malaria in the elimination phase—determinants and strategies: a cross-sectional study in rural China

    The current stage of malaria elimination in China requires experienced local health workers with sufficient knowledge of malaria who help to keep the public health system vigilant about a possible resurgence. ...

    Ruoxi Wang, Shangfeng Tang, Jun Yang, Tian Shao, Piaopiao Shao, Chunyan Liu, Da Feng, Hang Fu, Xiaoyu Chen, Tao Hu and Zhanchun Feng

    Malaria Journal 2017 16:210

    Published on: 19 May 2017

  19. Research

    Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs

    Plasmodium gametocytes are sexual stages transmitted to female Anopheles mosquitoes. While Plasmodium parasites can be differentiated microscopically on Giemsa-stained blood smears, mo...

    Amelia E. Hanron, Zachary P. Billman, Annette M. Seilie, Tayla M. Olsen, Matthew Fishbaugher, Ming Chang, Thomas Rueckle, Nicole Andenmatten, Bryan Greenhouse, Emmanuel Arinaitwe, John Rek, Smita Das, Gonzalo J. Domingo, Kelly Shipman, Stefan H. Kappe, James G. Kublin…

    Malaria Journal 2017 16:208

    Published on: 19 May 2017

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