Table 1 Polymerase Chain Reaction for amplification of fragments containing pfcrt and pfmdr1 gene polymorphisms
From: Molecular characterisation of drug-resistant Plasmodium falciparum from Thailand
Primer | Sequence (5' → 3') | PCR |
|---|---|---|
Pfcrt 76 | ||
1st round sense | CAAGAAGGAAGTAAGTATCCAAAAATGG | 94°C, 30''; 56°C, 30''; 60°C, 60''; 45 cycles |
Antisense | GTAGTTCTTGTAAGACCTATGAAGGC | |
Nested sense | GCAAAAATGACGAGCGTTATAGAG | 94°C, 30''; 59°C, 30''; 60°C, 60''; 45 cycles |
Antisense | CTGAACAGGCATCTAACATGGATATAGC | |
Pfmdr1 86 | ||
Sense | ATGGGTAAAGAGCAGAAAGAG | 94°C, 30''; 53°C, 30''; 68°C, 60''; 10 cycles, followed by 94°C, 30''; 50°C, 30''; 68°C, 60'', 35 cycles |
Antisense | CGTACCAATTCCTGAACTCAC | |
Pfmdr1 1042 | ||
1st round sense | TATGTCAAGCGGAGTTTTTGC | 94°C, 30''; 50°C, 30''; 68°C, 60''; 45 cycles |
Antisense | TCTGAATCTCCTTTTAAGGAC | |
Semi-nested sense | GTAAATGCAGCTTTATGGG | 94°C, 30''; 50°C, 30''; 68°C, 60''; 45 cycles |
Antisense | TCTGAATCTCCTTTTAAGGAC | |
Pfmdr1 1246 | ||
Sense | CTACAGCAATCGTTGGAGAAA | 94°C, 30''; 53°C, 30''; 68°C, 60''; 10 cycles, followed by 94°C, 30''; 50°C, 30''; 68°C, 60'', 35 cycles |
Antisense | GCTCTAGCTATAGCTATTCTC |