Preparation of pure and intact Plasmodium falciparum plasma membrane vesicles and partial characterisation of the plasma membrane ATPase

Table 1 Marker enzyme activities of isolated erythrocyte ghosts, trophozoites and parasite plasma membranes.

Samples	AchE specific activities (μmol/min/mg protein)	pLDH specific activities (μmol/min/mg protein)
Erythrocyte ghosts	2.30 ± 0.15	N.D.
Trophozoites	0.05 ± 0.03	0.14 ± 0.01
Parasite plasma membranes	No activity	No activity

Acetylcholine esterase (AchE) and parasite lactate dehydrogenase (pLDH) activities of isolated trophozoites, parasite plasma membranes and/or erythrocyte ghosts were determined as described under materials and methods section. Values are means ± standard deviation of three separate experiments from separate parasite plasma isolations, trophozoite isolations or erythrocyte membrane preparations. N.D.: not determined.

ISSN: 1475-2875