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Table 1 The degree of sequence homology between chosen peptide and variants within relatively conserved blocks of PfEMP1.

From: Cellular responses to Plasmodium falciparum erythrocyte membrane protein-1: use of relatively conserved synthetic peptide pools to determine CD4 T cell responses in malaria-exposed individuals in Benin, West Africa

 

Peptides in each pool

Length of peptide

Number of variants compared

Identity with variants

Similarity with variants

Pool 1 Peptides from DBLα region

 

11

10

1

1aa:11], 2aa:2, 5aa:4, 7aa:1, 8aa:1

 

GQCTFNRIKDS

12

10

7

1aa:3

  

12

11

0

2aa:4, 3aa:2, 4aa:4, 1aa:1

 

GACAPYRRLHLC

19

11

1

1aa:3, 2aa:7

 

HNLLADVCEAAK

17

11

1

1aa:2, 3aa:3, 1aa:2, 6 aa:2, 10aa:1

 

ARSFADIGDIVRGKDLYLG

19

11

4

1aa:2, 2aa:4, 3aa:1

 

LREDWWTANRETVWKAI

12

11

1

2aa:3, 3aa:1, 4aa:1, 6aa:2, 7aa:3

 

VPTYFDYVPQYLRWFEEWA

16

11

0

2aa:4, 3aa:3, 4aa:2, 5aa:1, 6aa:1

 

SRNGYDCTKTIR

15

11

0

2aa:2, 3aa:2, 4aa:1, 5aa:3, 6aa:1, 7aa:2

 

WIDNQKKEFLKQKKKY

    
 

TFSHTEYREPCPWCG

    

Pool 2 peptides from CIDRα and β and DBLβ-δ regions

KDSIEWRSKLSNCLK

15

11

1

5aa:2, 6aa:3, 7aa:1, 8aa:3, 9aa:1

 

CRAMKYSFADLGDII

15

6

1

1aa:1, 2aa:3, 4aa:1

 

ICIPPRRRRLYVGKL

15

10

0

1aa:1, 2aa:4, 3aa:1, 4aa:2, 6aa:1, 8aa:1

  

11

10

2

1aa:3, 2aa:3, 4aa:1, 8aa:1

 

SAAIETFFLWD

14

9

0

1aa:1, 2aa:1, 3aa:4, 4aa:2, 5aa:1

 

GVIPPDFLRQMFYT

15

10

1

3aa:5, 4aa:1, 5aa:1, 7aa:1, 10aa:1

 

IWNGMICALTYKEKD

12

10

4

1aa:2, 2aa:1, 3aa:1, 5aa:1, 7aa:1

 

RPPYFRYLEEWG

13

6

2

1aa:2, 2aa:2

 

WMTEWAEWFCKEQ

13

10

1

1aa:2, 5aa:1, 6aa:1 8aa:2, 9aa:1, 10aa:1,

 

FTYFYLKKKTKSS

   

11aa:1

 

IPTKLSPNRYIPYTSGKYRG-

27

10

4

1aa:1, 9aa:2, 22aa:1, 23aa:2

 

KRYIYLE

10

10

4

1aa:3, 6aa:2, 9aa:1

 

ESEYEEMDIN

9

7

5

4aa:2

 

DHYSDITSS

    

Pool 3 peptides from Exon 2 region

DIYVPGSPKYKTLIEVVLEPS

21

8

5

1aa:2, 4aa:1

  

15

10

1

2aa:4, 3aa:2, 4aa:1, 12 aa:2

 

ITDDEWNQLKKDFIS

11

8

2

1aa:2, 2aa:1, 6aa:1, 7aa:2

 

VDNNTHPTMSR

10

8

5

1aa:3

 

KPFIMSIHDR

14

9

3

1aa:3, 3aa:1, 7aa:1, 10aa:1

 

YSGIDLINDALSGN

19

9

0

1aa:6, 2aa:2, 14aa:1

 

HIDIYDELLKRKENELFGT

13

8

3

1aa:3, 2aa:2

 

NLFHKWLDRHRDM

11

8

2

1aa:2, 3aa:2, 5aa:1, 6aa:1

 

KLKELWENETH

10

7

2

1aa:2, 2aa:1, 4aa:1, 5aa:1

 

MDTILDDLEK

    
  1. 1] Indicates the number of variant peptides in a conserved block that differs from the peptide used for the T cell assays by the number of amino acids shown as "aa" (for example, 5aa:4 means that 4 variant peptides in a conserved block differ from the peptide used for the T cell assay by 5 amino acids.
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Malaria Journal

ISSN: 1475-2875

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