Figure 2

(A) Gene construction scheme for the expression of the MLC chimeric recombinant protein. LB and RB indicate the left and right borders of the T-DNA region, which is stably integrated into nuclear chromosomal DNA during Agrobacterium-mediated DNA delivery. TSS represents the translation start site, and SEKDEV (Serine-Glutamate-Lysine-Aspartate-Glutamate-Valine) stands for secretion signal. Additionally, this signal promotes exact oligomerization. The selectable marker used for plant transformation is the nptII gene, which confers kanamycin resistance. The GUS reporter protein was used as a marker to enable rapid screening in selective steps of plant regeneration. (B) Nucleotide and deduced amino acid sequence of the MLC chimeric recombinant gene. It is comprised of a modified PvMSP-1 (PvMSP1_ICB10, AF435594), a linker (Pro-Gly repeat, underlined), and PvCSP (PvCSP_TR, AF316582).