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Table 1 Molecular criteria for the classification of three genotypes observed in the malaria patient.

From: Chloroquine resistant vivax malaria in a pregnant woman on the western border of Thailand

Genetic Locus

Genotype A

Genotype B

Genotype C

Pvcs*

   

   Size (bp)

650

650

650

   Alu I (VK210-type)

D

D

D

   Bst NI (VK247-type)

U

U

U

   Scr FI/Bbs I

U/U

D/U

U/U

(VK210)

   

   Type

VK210 (i)

VK210 (ii)

VK210 (i)

Pvmsp1

   

   F1 fragment (bp)

400

400

400

Pvmsp3α*

   

   Alu I (bp)

560, 260, 190, 170

560, 460, 200, 180, 150, 120

560, 460, 200, 180, 150, 120

   Hha l (bp)

1100, 460, 260, 180

1100, 440, 260, 220

1100, 440, 260, 220

   Type

(i)

(ii)

(ii)

Pvmdr1 =

   

   T958M

M

M

M

   Y976F

F

F

Y

   F1076L

L

L

F

   Copy Number

1

1

1

   Type

(i)

(i)

(ii)

  1. * Following incubation with specific restriction enzyme the amplified Pvcs fragment is either digested (D) or remains uncut (U). Alu I and Bst NI digestion indicate whether the central polymorphic repeat region, which is amplified for the genotyping, is of the VK210 or VK247 type, while Scr FI and Bbs I digestion indicate the type of pre- and post-repeat pattern surrounding the VK210 repeat type allelic variants.
  2. With respect to Pvmsp3α, the pattern of fragments obtained following restriction enzyme digestion provides a means to distinguish between different parasite genotypes. Different allelic variants are denoted with (i) and (ii).
  3. = The amino acid residue encoded is provided, as is the average copy number. Different allelic variants are denoted with (i) and (ii).