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Table 1 Molecular criteria for the classification of three genotypes observed in the malaria patient.

From: Chloroquine resistant vivax malaria in a pregnant woman on the western border of Thailand

Genetic Locus Genotype A Genotype B Genotype C
Pvcs*    
   Size (bp) 650 650 650
   Alu I (VK210-type) D D D
   Bst NI (VK247-type) U U U
   Scr FI/Bbs I U/U D/U U/U
(VK210)    
   Type VK210 (i) VK210 (ii) VK210 (i)
Pvmsp1    
   F1 fragment (bp) 400 400 400
Pvmsp3α*    
   Alu I (bp) 560, 260, 190, 170 560, 460, 200, 180, 150, 120 560, 460, 200, 180, 150, 120
   Hha l (bp) 1100, 460, 260, 180 1100, 440, 260, 220 1100, 440, 260, 220
   Type (i) (ii) (ii)
Pvmdr1 =    
   T958M M M M
   Y976F F F Y
   F1076L L L F
   Copy Number 1 1 1
   Type (i) (i) (ii)
  1. * Following incubation with specific restriction enzyme the amplified Pvcs fragment is either digested (D) or remains uncut (U). Alu I and Bst NI digestion indicate whether the central polymorphic repeat region, which is amplified for the genotyping, is of the VK210 or VK247 type, while Scr FI and Bbs I digestion indicate the type of pre- and post-repeat pattern surrounding the VK210 repeat type allelic variants.
  2. With respect to Pvmsp3α, the pattern of fragments obtained following restriction enzyme digestion provides a means to distinguish between different parasite genotypes. Different allelic variants are denoted with (i) and (ii).
  3. = The amino acid residue encoded is provided, as is the average copy number. Different allelic variants are denoted with (i) and (ii).