Population genetic analysis of the Plasmodium falciparum 6-cys protein Pf38 in Papua New Guinea reveals domain-specific balancing selection

Table 1 Analysis of polymorphism and selection in Pf38 sequences (85-892 bp) in different P. falciparu m populations

										Codon based Z tests (p-value)			McDonald-Kreitman					Allele frequency based tests
	n	S^a	dN	dS	h	Hd	Π (×10³)	K (×10³)	HKAr	dN = dS	dN > dS	dN < dS	SYN		NONSYN			Tajima's D	Fu & Li F
													Fixed	Poly	Fixed	Poly	p-value
Full (85-892bp)
Mugil	36	10	9	0	11	0.88	3.24	18.97	0.17	0.014*	0.007*	1	4	0	9	9	0.115	0.331	0.303
Wosera	23	8	7	0	9	0.86	2.81	18.24	0.15	0.067	0.035*	1	4	0	9	7	0.248	0.151	-0.397
Gambia	88	13	11	1	36	0.95	4.04	19.09	0.21	0.015*	0.007*	1	4	1	9	11	0.321	0.432	1.057
Domain I (85-466bp)
Mugil	36	6	6	0	6	0.82	5.74	18.33	0.31	0.023*	0.012*	1	4	0	1	6	0.015*	1.407	1.475^#
Wosera	23	7	7	0	8	0.85	5.71	17.53	0.33	0.026*	0.012*	1	4	0	1	7	0.010*	0.475	0.108
Gambia	88	9	8	1	23	0.90	6.69	17.85	0.37	0.021*	0.009*	1	4	1	1	8	0.0229*	1.106	0.908
Domain II (467-892bp)
Mugil	36	4	3	0	5	0.35	0.99	19.55	0.05	0.101	0.052	1	0	0	8	3	n/a	-1.3773	-1.336
Wosera	23	1	n/a	n/a	2	0.09	0.20	18.88	0.01	0.295	1	0.151	0	0	8	0	n/a	-1.161	-1.640
Gambia	88	4	3	0	4	0.60	1.63	20.20	0.08	0.32	0.154	1	0	0	8	3	n/a	-0.247	0.668

*significant (p < 0.05) #aproaching significance (p < 0.10) n/a = not applicable
n = number of sequences, S = number of polymorphic sites, dN = nonsynonymous polymorphisms, dS = synonymous polymorphisms, h = haplotypes, Hd = Haplotype diversity, Π = nucleotide diversity, K = interspecific divergence, HKAr = HK, A ratio (Π/K)
^aThere is a polymorphism within an incomplete codon at the 3' end of the sequence, preventing determination of NS or Syn, which explains the lower number of dN+dS than S

ISSN: 1475-2875