Figure 3

Analysis of cross-linking site. A. PAGE analysis of LSA-NRC TG2-cross-linking in the absence (i) or presence (ii) of peptide corresponding to the major repeat sequence of LSA-1. B. RP-HPLC analysis of a peptide corresponding to the major repeat sequence of LSA-1 before (i) and after (ii) gpTG2 treatment for 2 h at 100 μg/ml gpTG2. Position of monomers [retention time 23.3 min] (1), dimers [retention time 24.5 min] (2) and trimers [retention time 25.6 min] (3) are indicated. (ii). C. Tertiary structure of a single LSA-1 major repeat as predicted by Robetta modeling. Arrows indicate glutamines and lysines predicted to be involved in TG2 mediated cross-linking. D. PAGE analysis of gpTG2 cross-linking of LSA-NRC-C (i) and LSA-NRC-N (ii). * indicates band formed by the gpTG2 enzyme (MW - 76.6 kDa).