Schematic representation of the life cycle of Plasmodium parasites showing a summary of inhibition and expression studies of plasmodial hexose transporters. Parasites shown in green represent a pbht-gfp transgenic line, which has been analysed via direct fluorescence imaging. Using this transgenic line, expression of PbHT-GFP was observed at 24, 48 and 67 h post infection in the liver stage , in both early and late asexual blood stages, in sexual blood stage forms (gametocytes) and in female gametes/or zygotes, ookinetes, midgut oocysts and sporozoites derived from mosquito midgut oocysts and salivary glands . Highlighted life cycle stages (blue boxes) show where compound 3361 inhibits parasite growth and development; in the liver stages in vitro, P. berghei parasite growth is inhibited by compound 3361 with an IC50 value of 11 μM ; in the asexual blood stages in vitro, P. falciparum parasite growth is inhibited by compound 3361 with an IC50 value of 16 μM and in vivo P. berghei parasite growth is reduced by 40% (4-day suppression test; 25 mg/kg; administered i.p. twice daily) ; in P. berghei sexual stages male gametogenesis (IC50 value of 286 μM) and ookinete production (IC50 value of 252 μM) are inhibited by compound 3361 .