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Table 1 Comparison of biochemical and kinetic properties of protozoan and mammalian hexose transporters

From: Plasmodial sugar transporters as anti-malarial drug targets and comparisons with other protozoa

 

Properties of hexose transport in parasites or host membranes

Characterization of hexose transporters genes in heterologous systems (Xenopus oocytes, CHO cells, Δlmgt)

Inhibition by compound 3361 Ki(mM)

Inhibition of glucose uptake by D-glucose analogues: important carbon position

Refs

 

K m for D-glucose or 2-DOG (mM)

K m for D-glucose or 2-DOG (mM)

Transport of D-fructose/K m (mM)

   

P. falciparum

(PfHT)

~5 mM (6-DOG)

0.97 ± 0.34

+/11.6

0.053 ± 0.02

1,3,6

[18, 30]

P. knowlesi (PkHT1)

ND

0.67 ± 0.16

+/5.12

0.108 ± 0.051

3,4,6

[30, 63]

P. vivax

(PvHT)

ND

0.52-0.85

+/5.91-19.03

0.112 ± 0.13

3,4,6

[30, 63]

P. yoelii

(PyHT1)

ND

0.12 ± 0.04

+/2.94

0.080 ± 0.016

3,4,6

[30, 63]

P. berghei

(PbHT)

ND

0.09 ± 0.02

0.54

~ 0.009

ND

[40]

T. gondii (TgGT1)

ND

0.030 ± 0.006

+ §

2.073 ± 0.59

3,4,6

[63]

B. bovis (BboHT1)

ND

0.84 ± 0.50

+/ND

0.005 ± 0.002

4,6

[58]

Leishmania LmGT1

1.22 ± 0.22

ND

+/ND

ND

ND

[56]

Leishmania LmGT2

109 ± 22

ND

+/ND

ND

ND

[56]

Leishmania LmGT3

208 ± 40

ND

+/ND

ND

ND

[56]

T. brucei

THT1

0.5-2.0

ND

+/2.56-3.91

ND

3,4

[55, 64–66]

T. brucei

THT2

0.038-0.08/2.0

0.151 ± 0.01

+/2.54

ND

3,4,6

[66]

T. vivax

TvHT1

0.585 ± 0.068

0.545 ± 0.02

+/ND

ND

1,3,6

[65–67]

GLUT1

4-10

~ 2

-

3.3 ± 0.21

1,4,6

[19, 66]

GLUT5

ND

-

+/13

ND

ND

[68]

  1. ND - not determined
  2. § - D-fructose uptake at 37°C
  3. +/ND - transport of fructose observed but Km not determined
  4. Δlmgt - glucose transporter null mutant of Leishmania mexicana
  5. CHO - The Chinese hamster ovary (CHO) cell line