Example of prozone for Paracheck-Pf. Footnotes figure 2: Paracheck-Pf RDT cassettes run with a blood sample infected with P. falciparum at a parasite density of 8.3%. The sample was assessed in duplicate, undiluted (P25 A and P25 B), 10 × diluted (P25 × 10A and P25 × 10B) and 100 × diluted (P25 × 100A and P25 × 100B). All cassettes show regular control lines, cassettes P25 A and B show no visible test line. Cassettes P25 × 10A and P25 × 10B show weak test lines and the maximum line intensity (strong) was obtained after 100 × dilution (cassettes P25 × 100A and P25 × 100B).