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Table 1 The frequency of positive ELISA activities conducted in urban and rural volunteers with 7 recombinant proteins representing some candidate vaccine antigens

From: Measuring naturally acquired immune responses to candidate malaria vaccine antigens in Ghanaian adults

Method

  

Antigens tested

  

1

Site

No. vol

CSP

TRAP

EXP1

LSA1

MSP1

MSP3

EBA

Total

Tot/v

 

Urban

14

2* (14%)

10 (71%)

11 (79%)

12 (86%)

11 (79%)

14 (100%)

8 (57%)

69

4.9

 

Rural

21

11 (52%)

21 (100%)

19 (90%)

20 (95%)

17 (81%)

20 (95%)

16 (90%)

124

5.9

 

Total

35

13 (37%)

31 (89%)

30 (86%)

32 (91%)

28 (80%)

34 (97%)

24 (86%)

192

5.5

2

Urban

14

0 (0%)

4 (29%)

5 (36%)

12 (86%)

10 (71%)

14 (100%)

2 (14%)

47

3.4

 

Rural

21

5 (24%)

10 (48%)

10 (48%)

19 (90%)

14 (67%)

19 (90%)

5 (24%)

82

3.9

 

Total

35

5 (14%)

14 (40%)

15 (43%)

31 (89%)

24 (69%)

33 (94%)

7 (20%)

129

3.7

  1. Positive ELISA activities were defined using method 1 (OD that was above the mean of control negative donors (Danish volunteers with no previous exposure to malaria) + 3 SD) or method 2 (OD ≥ 0.5 at a dilution of >1/100 and greater activity than control sera + 3 SD). The Table shows the numbers of urban and rural volunteers that were positive with each antigen. The total numbers of antigens positive with urban and rural volunteers, and the frequency of positive antigens/volunteer (Tot/v) are shown in the two right columns. * Number of volunteers positive.