Measuring naturally acquired immune responses to candidate malaria vaccine antigens in Ghanaian adults

Malaria Journal

Table 6 Part B assay schedule

	CSP SAMPLE 1			AMA SAMPLE 1			CSP SAMPLE 2			AMA SAMPLE 2
Day	0	0	0	9	9	9	14	14	14	21	21	21
Assay	1	2	3	1	2	3	1	2	3	1	2	3	TOTAL
1001				X	X	X				X	X	X	6
1024	X	X	X	X	X	X	X	X	X	X	X	X	12
1036	X	X	X	X		X	X	X	X	X	X	X	11
1039	X	X	X	X	X	X	X	X	X	X	X	X	12
1041	X	X	X				X	X	X				6
1054	X	X	X	X	X	X	X	X	X	X	X	X	12
1056	X	X	X	X	X	X	X	X	X	X	X	X	12
TOTAL	6	6	6	6	5	6	6	6	6	6	6	6	71

Each volunteer was sampled at different time points (for CSP day 0 and day 14; AMA1 day 9 and day 21). Frozen PBMC from each time point were tested in ELISpot assays with CSP or AMA peptide pools in three assays 1, 2 and 3 on different days as indicated. Assays used for analysis are boxed. X indicates where that volunteer was tested with CSP or AMA1 pools. The seven volunteers in the table were analysed for ELISpot reproducibility at each of the two time points (Supplementary Tables 1, 2 and 3). Totals refer to the numbers of assays used in this analysis. An additional five volunteers enrolled in the study and tested at some time points were excluded from this table and from the consistency analysis because data sets were not sufficiently complete.

ISSN: 1475-2875