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Figure 2 | Malaria Journal

Figure 2

From: Gestational malaria associated to Plasmodium vivax and Plasmodium falciparum placental mixed-infection followed by foetal loss: a case report from an unstable transmission area in Brazil

Figure 2

The presence of P. falciparum- and P. vivax -infected erythrocytes in the placenta collected after foetal loss. Agarose gel electrophoresis of nested-PCR amplified products in the presence of species-specific human malaria parasite oligonucleotides (VIV or FAL, specific for P. vivax or P. falciparum, respectively) based on the parasite small subunit ribosomal RNA (ssrRNA) gene. Both P. vivax and P. falciparum gDNA were found in the placenta; no plasmodial gDNA was detected in peripheral blood after delivery. Abbreviations are as follows: MW, molecular weight; bp, base pair; B, patient peripheral blood sample; niB, non-infected human peripheral blood; P, patient placenta sample; niP, non-infected human placenta; C-, negative control, absence of nuclear material; Pv+ and Pf+, positive controls representing amplification product of P. vivax (120 bp) and P. falciparum (200 bp) ssrRNA gene.

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Malaria Journal

ISSN: 1475-2875

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