Anti-FVO AMA1 IgG titres in sera of animals immunized with AMA1-based vaccines. Three groups of six rhesus monkeys each were immunized with Pf AMA1-based formulations on days 0, 28 and 56. One group was immunized with DiCo mix formulated in CoVaccine HT™ (DiCo/HT), the second group with DiCo mix in Montanide ISA 51 (DiCo/ISA), and the third group with an AMA1-MSP119 fusion protein in Montanide ISA 51 (AM/ISA). IgG titres in samples taken prior to each immunization, as well as in samples drawn on days 14, 42, 70, 99 and 126, were measured by an indirect ELISA. Similar patterns were observed for IgG measurements against six other Pf AMA1 alleles (vaccine antigens DiCo 1, DiCo 2 and DiCo 3 as well as Pf AMA1 from the HB3, 3D7 and CAMP parasite strains). For each panel, the different colours/symbols represent individual animals.