Expression of Plasmodium vivax proteins in cell-free systems. A. Expression of proteins in the E. coli cell-free system. B. Expression of proteins in the wheat germ cell-free system. Both extracts were separated in soluble and insoluble protein fractions by centrifugation (14,000 g/20 mim/4°C). Soluble fractions were analysed by Western blot using anti-GST (HRP) antibody. Molecular weights in kilo-Daltons are indicated to the left and soluble Pv-fusion proteins of predicted sizes are marked with an arrow.