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Figure 5 | Malaria Journal

Figure 5

From: Plasmodium vivax: comparison of immunogenicity among proteins expressed in the cell-free systems of Escherichia coli and wheat germ by suspension array assays

Figure 5

Comparative analysis of immune responses to P. vivax proteins expressed in the E. coli and wheat germ cell-free systems by Bioplex. One μg of each affinity-purified protein was individually coupled to beads and analysed by multiplex assays using immune sera (1:100 dilution) pertaining to 40 different P. vivax patients. Fluorescence was determined as the mean fluorescence intensity (MFI). GST values were subtracted from MFI values obtained against individual recombinant proteins and the cut-off defined as the mean value of control sera + 3 standard deviations. Circles represent samples which MFI values were below the cut-off and were considered negative whereas squares represent samples which MFI values were above the cut-off and were considered positive. Geometric means and 95% confidence intervals are shown.

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