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Figure 4 | Malaria Journal

Figure 4

From: Plasmodium falciparum glutamate dehydrogenase a is dispensable and not a drug target during erythrocytic development

Figure 4

Western blot analysis of P. falciparum proteins involved in antioxidant defence, redox balance or NADP(H) production. The left panel shows representative western blots of D10, D10Δgdha-1 and D10Δgdha-2 protein extracts (10 μg) with a range of specific antibodies. The loading control is shown underneath each blot and is either an antibody against the E2 subunit of branched chain α- ketoacid dehydrogenase (50 kDa, panel A - G) or 2Cys- peroxiredoxin (25 kDa, panel H). The two panels on the right show the respective graphs (means ± S.D.) of the densitometry analyses of two or three independent protein extracts. Relative protein levels were calculated by comparing the intensity of the specific protein band with the loading control, with D10 set at 100% (cl1 is D10Δgdha-1 and cl2 is D10Δgdha-2). A) glutathione reductase (GR, 56 kDa), B) thioredoxin 1 (Trx, 12 kDa), C) blot B re-probed with glutathione S-transferase antiserum (GST, 25 kDa), D) superoxide dismutase 1 (SOD1, 22 kDa), E) superoxide dismutase 2 (SOD2, 22 kDa), F) 1Cys-peroxiredoxin (1Cys-Px, 25 kDa), G) 2Cys-peroxiredoxin (2Cys-Px, 25 kDa), H) isocitrate dehydrogenase (IsoDH, 54 kDa).

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