Plasmodium falciparum glutamate dehydrogenase a is dispensable and not a drug target during erythrocytic development

Malaria Journal

Table 1 IC₅₀ values for oxidative stress inducers and an aminotransferase inhibitor for D10 and D10^Δgdhaclones

		IC₅₀values (μM)
Parasite line	N-methylphenazonium methosulfate	*Tert* -butylhydroperoxide	L-cycloserine
D10	0.8 ± 0.2	76.7 ± 20.0	145.8 ± 56.6
D10^Δgdha-1	0.7 ± 0.2	81.8 ± 17.2	113.5 ± 37.5
D10^Δgdha-2	0.7 ± 0.1	81.3 ± 15.5	111.9 ± 37.4

The effect on parasite viability by the endogenous stressor N-methylphenazonium methosulfate, the exogenous stressor tert-butylhydroperoxide and the aminotransferase inhibitor L-cycloserine were determined by the incorporation of [³H]-hypoxanthine in the presence of increasing drug concentrations. The IC₅₀ values were calculated by non linear regressing of the sigmoidal dose-response equation and shown are the mean ± S.D. of two (N-methylphenazonium methosulfate) or three (tert-butylhydroperoxide) independent experiments. For L-cycloserine the mean ± S.E.M of six independent experiments is shown.