Skip to main content

Advertisement

Table 2 Quantitative real time PCR of relevant genes from D10 and D10Δgdhaclones

From: Plasmodium falciparum glutamate dehydrogenase a is dispensable and not a drug target during erythrocytic development

Fold increase of mRNA compared to D10
Parasite line g6pdh gdhb gdhc gs γgcs
D10Δgdha-1 1.8 ± 0.7 1.6 ± 0.2 1.4 ± 0.3 1.4 ± 0.3 2.1 ± 0.2*
D10Δgdha-2 2.4 ± 1.0 2.2 ± 0.3* 1.7 ± 0.3 1.5 ± 0.4 2.8 ± 0.2*
  1. The amount of mRNA was calculated using seryl-t-RNA synthase as endogenous control and normalized to the amount of mRNA in D10. The values are the mean ± S.D. of two independent experiments. * denotes a significant difference in expression level compared to D10 (p < 0.05).