Skip to main content

Table 2 Quantitative real time PCR of relevant genes from D10 and D10Δgdhaclones

From: Plasmodium falciparum glutamate dehydrogenase a is dispensable and not a drug target during erythrocytic development

Fold increase of mRNA compared to D10

Parasite line

g6pdh

gdhb

gdhc

gs

γgcs

D10Δgdha-1

1.8 ± 0.7

1.6 ± 0.2

1.4 ± 0.3

1.4 ± 0.3

2.1 ± 0.2*

D10Δgdha-2

2.4 ± 1.0

2.2 ± 0.3*

1.7 ± 0.3

1.5 ± 0.4

2.8 ± 0.2*

  1. The amount of mRNA was calculated using seryl-t-RNA synthase as endogenous control and normalized to the amount of mRNA in D10. The values are the mean ± S.D. of two independent experiments. * denotes a significant difference in expression level compared to D10 (p < 0.05).
Back to article page

Malaria Journal

ISSN: 1475-2875

Contact us