Plasmodium falciparum glutamate dehydrogenase a is dispensable and not a drug target during erythrocytic development

Table 2 Quantitative real time PCR of relevant genes from D10 and D10^Δgdhaclones

Fold increase of mRNA compared to D10
Parasite line	g6pdh	gdhb	gdhc	gs	γgcs
D10^Δgdha-1	1.8 ± 0.7	1.6 ± 0.2	1.4 ± 0.3	1.4 ± 0.3	2.1 ± 0.2*
D10^Δgdha-2	2.4 ± 1.0	2.2 ± 0.3*	1.7 ± 0.3	1.5 ± 0.4	2.8 ± 0.2*

The amount of mRNA was calculated using seryl-t-RNA synthase as endogenous control and normalized to the amount of mRNA in D10. The values are the mean ± S.D. of two independent experiments. * denotes a significant difference in expression level compared to D10 (p < 0.05).

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