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Table 3 Metabolic labelling resulting from growth of D10 and D10Δgdhaclones with U-[13C] glutamine-containing medium

From: Plasmodium falciparum glutamate dehydrogenase a is dispensable and not a drug target during erythrocytic development

Percentage incorporation of [13C]
Parasite line +5-[13C] glutamate +5-[13C] α-ketoglutarate +4-[13C] succinate
rbc 60.5 ± 1.1 0 4.9 ± 1.4
D10 48.8 ± 1.8 47.1 ± 3.4 41.6 ± 3.0
D10Δgdha-1 45.9 ± 0.8 49.7 ± 4.1 39.9 ± 2.6
D10Δgdha-2 45.9 ± 0.6 53.0 ± 3.3 33.4 ± 5.5
  1. The parasite lines were cultured for 32 h with medium containing 2 mM U-[13C] glutamine and metabolites extracted and analysed by LC-MS. The percentage of metabolite which has [13C] incorporated is shown. The values are the mean ± S.D. of three independent experiments, which were done in triplicate.