False positive circumsporozoite protein ELISA: a challenge for the estimation of the entomological inoculation rate of malaria and for vector incrimination

Table 4 CSP-ELISA positive mosquitoes as reported in Trung et al [28] confirmed by Plasmodium spp. PCR

Village	Species	Number of mosquitoes positive by Plasmodium specific PCR/number of CSP-ELISA positive mosquitoes tested by PCR
		P. falciparum	P. vivax
Lang Nhot (Khanh Hoa)	An. minimus	3/3	1/1
	An. dirus	1/1	0
Village 3 (Binh Thuan)	An. dirus	2/3 *	0
Cha Ong Chan (Rattanakiry)	An. minimus	1/1	0
	An. dirus	1/1 ^a	0/1 ^a

* The PCR negative sample gave a very weak signal when tested by mosquito specific ITS2 PCR, meaning that the DNA was not optimal anymore. This sample cannot be considered as CSP-ELISA false positive.
^a P. falciparum and P. vivax were detected by ELISA in the same mosquito. Sequencing of the PCR product only revealed P. falciparum.

ISSN: 1475-2875