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Table 6 ELISA results for the abdomens of specimens with true and false positive P.falciparum ELISA results of the head-thoracic portions

From: False positive circumsporozoite protein ELISA: a challenge for the estimation of the entomological inoculation rate of malaria and for vector incrimination

 

Abdomens of P. falciparum ELISA true positive mosquitoes 1

Abdomens of P. falciparum ELISA false positive mosquitoes 2

 

PCR +

PCR -

Total

PCR +

PCR -

Total

ELISA score 0

3

4

7

0

143

143

ELISA score 1

5

0

5

0

3

3

ELISA score 2

3

0

3

0

1

1

ELISA score 3

4

0

4

0

0

0

Total

15

4

19

0

147

147

  1. 1 For all abdomens of true positive mosquitoes, the ELISA score remained the same after heating the specimen for 10 minutes at 100°C.
  2. 2 For all abdomens of false positive mosquitoes, the ELISA score turned to zero after heating the specimen for 10 minutes at 100°C.