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Table 6 ELISA results for the abdomens of specimens with true and false positive P.falciparum ELISA results of the head-thoracic portions

From: False positive circumsporozoite protein ELISA: a challenge for the estimation of the entomological inoculation rate of malaria and for vector incrimination

  Abdomens of P. falciparum ELISA true positive mosquitoes 1 Abdomens of P. falciparum ELISA false positive mosquitoes 2
  PCR + PCR - Total PCR + PCR - Total
ELISA score 0 3 4 7 0 143 143
ELISA score 1 5 0 5 0 3 3
ELISA score 2 3 0 3 0 1 1
ELISA score 3 4 0 4 0 0 0
Total 15 4 19 0 147 147
  1. 1 For all abdomens of true positive mosquitoes, the ELISA score remained the same after heating the specimen for 10 minutes at 100°C.
  2. 2 For all abdomens of false positive mosquitoes, the ELISA score turned to zero after heating the specimen for 10 minutes at 100°C.