Specific, sensitive and rapid detection of human plasmodium knowlesi infection by loop-mediated isothermal amplification (LAMP) in blood samples

Table 2 Results of LAMP compared to microscopy and nested PCR for detecting P. knowlesi

Microscopy/nested PCR result	LAMP result		Microscopy/nested PCR total
	P. knowlesi	Negative
Microscopy result
P. knowlesi or P. malariae	12	1^a	13
P. knowlesi or P. falciparum	1^c	1^b	2
Non-knowlesi	0	39	39
Negative	0	20	20
Nested PCR result
P. knowlesi	12	0	12
Non-knowlesi	0	41	41
Negative	1^c	20	21
LAMP total	13	61	74

^a Sample positive for P. malariae by nested PCR
^b Sample positive for P. falciparum by nested PCR
^c Sample positive for either P. knowlesi or P. falciparum by microscopy, with parasitaemia less than 0.01%

ISSN: 1475-2875