Global proteomic analysis of plasma from mice infected with Plasmodium berghei ANKA using two dimensional gel electrophoresis and matrix assisted laser desorption ionization-time of flight mass spectrometry

Gitau, Evelyn N; Kokwaro, Gilbert O; Newton, Charles RJC; Ward, Stephen A

doi:10.1186/1475-2875-10-205

Table 1 List of proteins identified from the Coomassie stained gels of plasma from infected mice (Figure 2)

From: Global proteomic analysis of plasma from mice infected with Plasmodium berghei ANKA using two dimensional gel electrophoresis and matrix assisted laser desorption ionization-time of flight mass spectrometry

Spot No.	Description	Accession	Score	Matched Peptides	Sequence coverage(%)
1	serine (or cysteine) proteinase inhibitor, clade A, member 1a; serine protease [Mus Musculus]	15029662	83	8	26
12, 14	similar to macrophage galactose-type C-type lectin 2 [Mus Musculus]	38089618	78, 68	7, 6	21, 20
36	similar to spectrin alpha chain, brain (spectrin, non-erythrid alpha chain)	38074605	71	10
42	60S ribosomal protein L35a	3914537	70	5	31
48, 50, 56, 72, 94, 95	apolipoprotein A-1 precursor - mouse	109571	137, 102,87,72,64,65	11, 9, 8, 7, 7, 6	34, 29, 27, 23, 19, 19
48, 50, 56, 67, 72, 94, 95	unamed protein product [Mus Musculus]	26345182	136, 102, 87, 76, 72, 64, 65	11, 9, 8, 8, 7, 7, 6	34, 29, 27, 28, 23, 19, 19
48, 50, 56, 94, 95	apolipoprotein A-1 [Mus Musculus]	2145135	119, 86, 72, 64, 65	10, 8, 7, 7, 6	29, 24, 22, 19, 19
48, 94	apolipoprotein A1 homolog [mus sp.]	1245804	69, 66	5, 7	53, 53
55	unamed protein product [Mus Musculus]	26341396	80	9	20
55	albumin 1; serum albumin variant [Mus Musculus]	33859506	78	9	19
55	serum albumin precursor	5915682	78	9	19
65	similar to 60S Ribsomal protein L29 (P23) [Rattus Norvegicus]	34866986	67	6	28
66	RNA binding motif protein, X chromosome [Mus Musculus]	6755296	72	8	16
66, 103	heterogenous nuclear ribonucleoprotein G [Mus Musculus]	5579009	68, 67	7, 7	20, 15
66	unamed protein product [Mus Musculus]	26339834	66	9	10
80	similar to 60S Ribsomal protein L29 (P23) [Mus Musculus]	38089196	78	7	25
80	similar to 60S Ribsomal protein L29 (P23) [Rattus Norvegicus]	34881340	71	7	30
81	unamed protein product [Mus Musculus]	26335691	69	7	25
81	heparan sulfate 6-0-sulfotransferase 1 [Mus Musculus]	20845347	64	6	28
82	similar to HRPAP20 short form [Rattus Norvegicus]	34867098	65	6	20
99	similar to Rp17a protein [Rattus Norvegicus]	34868324	86	9	16

The MALDI spectra were searched against the NCBInr database using the Mascot^® search algorithm. Proteins with a significant score as defined by Mascot^® were included in the list.

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ISSN: 1475-2875

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