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Table 2 Results of identification of Anopheles gambiae s.s. indoor-resting female samples by different genotyping approaches

From: Comparative analyses reveal discrepancies among results of commonly used methods for Anopheles gambiae molecular form identification

Samples PCR-RFLPs    Sequencing
  IGS581 IGS690 AS-PCR N (n) IGS581 IGS690
  1 MM MM MM 5 (4) T A
  2 MM MM MS 6 (3) T A
  3 MM MS MS 1 T A
  4 MM MS MS 6 T/C A/T
  5 MS MS MS 29 (28) T/C A/T
GUINEA BISSAU 6 MS SS MS 6 T/C A/T
  7 SS SS MS 2 T/C A/T
  8 MS SS MS 1 C T
  9 MS SS SS 4 C T
  10 SS SS SS 7 C T
  11 MM MM MM 9 (4) T A
  12 MM MM MS 7 (4) T A
  13 MM MS MM 1 T A
  14 MM MS MS 1 T A
THE GAMBIA 15 MM MS MS 2 T/C A/T
  16 MS MS MS 16 (8) T/C A/T
  17 MS SS MS 1 T/C A/T
  18 MS SS MS 2 C T
  19 SS SS MS 1 C T
  20 SS SS SS 9 (5) C T
  1. PCR-RFLP581[16], PCR-RFLP690 [17] and AS-PCR [13] genotyping and sequencing of IGS amplicon. IGS581: M-form = T, S-form = C; IGS690: M-form = A, S-form = T. N = numbers of specimens identified by PCR-RFLPs and AS-PCR. (n) = number of specimens sequenced, when these do not correspond to N.