Figure 1
From: High-speed shaking of frozen blood clots for extraction of human and malaria parasite DNA
Genotyping of the α-thalassaemia gene in blood clot samples from Kilifi District Kenya processed with high speed shaking and vortexing. Agarose gel showing PCR amplicons of different sizes used in the diagnosis of α-3.7 thalassaemia. The heaviest amplicon is a 2.5 kb fragment representing the lis 1 gene, which is used as a control for amplification success. The lanes M represents the molecular weight ladder; 1-18, amplification of clot DNA extracted using the high speed shaking method; 19-37, amplification of clot DNA extracted using the vortexing method; 38-40, positive controls; 41; DNA negative control.