Table 1 Sensitivity of PCR detection of malaria parasites in clots prepared with different parasite densities processed with three methods for clot dispersion
From: High-speed shaking of frozen blood clots for extraction of human and malaria parasite DNA
Method for clot disruption | ||||||
|---|---|---|---|---|---|---|
Parasite density (parasites/μl) | Chemical PG04 | Clotspin®Basket | High-speed shaking | |||
Nr PCR positive runs | Yield (ng/ul) | Nr PCR positive runs | Yield (ng/ul) | Nr PCR positive runs | Yield (ng/ul) | |
1000 | 5/5 | 1.0 | 5/5 | 2.2 | 5/5 | 26.4 |
100 | 1/5 | 0.7 | 2/5 | 3.3 | 5/5 | 11.5 |
50 | 2/5 | 3.5 | 1/5 | 2.7 | 5/5 | 2.4 |
10 | 0/5 | 0.3 | 1/5 | 5.5 | 4/5 | 2.5 |
1 | 0/5 | 1.5 | 0/5 | 1.9 | 0/5 | ND |