Rapid whole genome optical mapping of Plasmodium falciparum

Malaria Journal

Table 1 Summary of Results

Genome	Total Length (Mb)	Geographic Area	Variations to Reference^a	Maximum Single Variation^b
3D7 Reference	23.264	West Africa	N/A	N/A
3D7 Optical Map	23.165	West Africa	4	46.0Kb
D6	21.958	East Africa	5	43.6Kb
FVO	22.158	Southeast Asia	4	41.4Kb
C235	21.734	Southeast Asia	5	77.5Kb

The total megabases (Mb) of each genome predicted from alignment against the in silico optical map (or reference), the geographic area each strain was isolated from, the number of variations from each map to the reference genome and the largest (in Kb) observed difference. Variation under 2Kb and telomeric DNA were not included.
^arepresents independent differences observed against the reference genome using default assembly parameters. Results can vary by settings.
^bthe maximum number of kilobases for a single genomic event outlined in the study as compared to the in silico reference genome.

ISSN: 1475-2875