The antibody response to Plasmodium falciparum Merozoite Surface Protein 4: comparative assessment of specificity and growth inhibitory antibody activity to infection-acquired and immunization-induced epitopes

Table 1 Properties of MSP4-specific Mabs

Mab	Isotype^a	Recognition^b		Reduction	Relative
		Fragment	Peptide	Sensitivity^c	Affinity^d
H12	IgG1	rMSP4A	MSP4.2	*	ND
1B7	IgG1	rMSP4B	none	-	2.2
6G10	IgG1	rMSP4C	MSP4.13	-	2.1
4C10	IgG1	rMSP4D	MSP4.20	+	1.7
1G10	IgG1	rMSP4D	MSP4.20	+	1.7
7F4	IgG1	rMSP4D	none	+	1.4
8G3	IgG1	rMSP4D	none	+	1.4
1H2	IgG1	rMSP4D	MSP4.20	-	1.1
6G11	IgG1	rMSP4D	MSP4.20	-	1.6

a) Antibody isotype by ELISA. b) Fragment specificity by immunoblotting and ELISA and peptide recognition by ELISA. c) Reduction sensitivity measured as lack of reactivity (+) to reduced or reduced and alkylated MSP4. *mAb H12 only recognized reduced MSP4. d) Antibody affinity determined by ability of thiocyanate to interfere with antigen-antibody binding. ND: not determined.

ISSN: 1475-2875