Figure 1

IFN-γ is associated with protection induced by LSA3 in Aotus monkeys. Specific IFN-γ responses were studied in nine Aotus immunized either by LSA3 recombinant proteins [53] or by long peptides [54]. Monkeys were challenged with P. falciparum sporozoites from Santa lucia strain and the IFN-γ responses were evaluated in protected, non-protected and control animals immunized with the GST or ASO2 adjuvant alone. After immunization, the production of IFN-γ was assessed either ex-vivo by ELISpot or by ELISA after 40 h of in vitro re-stimulation with LSA3 peptides (squares) or Rc proteins (circles), as described in detail in [53] and [55].