Figure 6

Phylogenetic tree representing frequency of tissues within subgroups of the cir subfamily 2. Phylogenetic tree based on maximum likelihood estimation showing 216 protein sequences deduced from cir cDNAs of subfamily 2 amplified from six different host tissues of a single female NMRI mouse infected with 100 pRBCs (parasitaemia about 30%). The RT-PCR products for each tissue (blood, liver, spleen, kidney, lung and heart) were cloned and 36 clones from each tissue were sequenced. Statistical support is indicated as likelihood ratios only for those nodes with at least 90% support. The scale bar represents 0.1 substitutions per amino acid position. The origins of sequences (host tissues) are indicated by different colours as shown in the figure graphic legend. The cir subfamily 2 repertoire expressed here can be divided in 7 subgroups but only three of these subgroups (subgroup 2.5, 2.6, 2.7) showed significant differences of the frequency between tissues. The p-values were calculated by Z-test; **p < 0.01; *p < 0.05; ⁺p < 0.07). Small (rarely occurring) subgroups of cir cDNAs were excluded from the statistical analysis. For identification of the individual sequences in each branch see Additional file 8.