Table 1 Recombinant HRP2 concentrations used in Trial 1, and equivalent parasite densities derived from the median HRP2 concentration of dilutions of 200 parasite/μL of the WHO global specimen bank
HRP2 concentration (ng/mL) | Line intensity (densitometer) | Mean line intensity (3 readers) | Calculated parasite density equivalent (parasite/μL) |
|---|---|---|---|
15 | 1.8 | 1.3 | 313 |
150 | 7.2 | 3.0 | 3,125 |
1,500 | 16.9 | 4.0 | 31,250 |
15,000 | 17.9 | 4.0 | 312,500 |
150,000 | 13.0 | 3.7 | 3,125,000 |
300,000 | 10.8 | 3.0 | 6,250,000 |
500,000 | 5.2 | 2.3 | 10,416,667 |
750,000 | 4.4 | 2.0 | 15,625,000 |
1,000,000 | 2.4 | 2.0 | 20,833,333 |
1,500,000 | 1.5 | 1.0 | 31,250,000 |