Sub-cellular localization of the Pv RON1 protein in mature schizonts using fluorescence microscopy . (A) Apical localization of Pv RON1 protein. (1) Nuclei stained with DAPI; (2) Detection with anti-Pv RON1; (3) Overlapping of the two recognition patterns. (B) Double stained assay. (1) Nuclei stained with DAPI. (2) Recognition by anti-Pv RON1. (3) Detection of Pv RhopH3. (4) Overlapping of 1, 2 and 3 images.