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Figure 1 | Malaria Journal

Figure 1

From: A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance

Figure 1

Extracted ion chromatograms of transitions derived from peptide SHMT-4. The ordinate represents the peak intensity (normalised to 100%) and the abscissa the time within the elution gradient (minutes) that this intensity was detected. Panel A, transitions corresponding to the labelled version of this peptide; Panel B, transitions corresponding to the unlabelled version of this peptide. The relative area under each of the traces and the known amount of the standard (labelled) peptide added were used to calculate the amount of the unlabelled peptide and thus the amount of the equivalent protein present in the sample analyzed.

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