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Table 1 Quantification of P. falciparum proteins from two unsynchronized erythrocytic-stage cultures

From: A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance

Peptide

Fragment Ion Type

Calculated Native Level (Culture 1)

Peptide Average

Calculated Native Level (Culture 2)

Peptide Average

Average Between Samples

Adenosine Deaminase-1

y4

5.96

6.53 (0.23)

-

-

-

 

y5

6.83

 

-

  
 

y6

6.79

 

-

  

Disulphide Isomerase-1

y5

9.54

16.47 (2.83)

-

-

-

 

y6

20.11

 

-

  
 

y7

19.76

 

-

  

DHFR-TS-4

y7

0.68

0.31 (0.15)

0.24

0.68 (0.20)

0.50 (0.02)

 

y8

0.05

 

1.09

  
 

y10

0.21

 

0.72

  

eIF5α-3

y6

18.64

19.05 (1.90)

20.33

22.98 (2.13)

21.02 (1.39)

 

y8

15.23

 

28.20

  
 

y9

23.27

 

20.41

  

SHMT-4

y5

0.48

0.87 (0.31)

0.69

0.99 (0.29)

0.93 (0.04)

 

y6

0.49

 

1.69

  
 

y7

1.63

 

0.58

  
  1. All figures are pmol per 100 μg of P. falciparum protein and numbers in brackets represent standard errors. Adenosine Deaminase-1 and Disulphide Isomerase-1 were only detected in experiment 1.
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Malaria Journal

ISSN: 1475-2875

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