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Table 1 Effect of cofactors on Vmax of ME activity

From: Mitochondrial NAD+-dependent malic enzyme from Anopheles stephensi: a possible novel target for malaria mosquito control

Addition

K m for malate

(mM)

V max

nmol × (min × mg protein)-1

p-value to controls

None

-

3 ± 1

-

MgCl2 (3 mM)

-

24 ± 2

0.02

MnCl2 (3 mM)

0.12

56 ± 3 (20 2)

0.01

  1. All kinetic parameters were obtained with NAD+ except the V max obtained with NADP+ (value in parentheses). The V max values were expressed as mean ± SEM. All other experimental details are described in the Methods.