Figure 5

OPLS-DA scores plot of metabolite profiles based on ¹H NMR spectra of sera and pleural effusion of C57BL/6 mice with CM, and OPLS-DA coefficient plot of pleural effusion and sera of CM. The key to the peaks are given in Figure 2. (A) Each red circles denotes score of one ¹H CPMG NMR of pleural effusion and the black circle denotes score of one ¹H CPMG NMR of serum of CM C57BL/6 mouse at day 9 p.i in an OPLS-DA analysis. The ellipse is a 95% Hotelling T². R2X (cum) = 0.76, Q2 (cum) = 0.85. (B). The color of the signals in this plot signifies the contribution of the metabolites towards class segregation between PE and sera of CM C57BL/6. A color bar associated to the plot indicates the correlation of the metabolites in segregating between classes (CM sera and CM pleural effusion) with least significance of color black and highest significance of yellow color. The upward orientation of the peaks denotes relatively higher concentration of the corresponding metabolites in sera and vice-versa.