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Figure 2 | Malaria Journal

Figure 2

From: In vitro culture of Plasmodium berghei-ANKA maintains infectivity of mouse erythrocytes inducing cerebral malaria

Figure 2

In vitro reinvasion rate of mouse erythrocytes by P. berghei. A/Effect of the storage of P. berghei ANKA before seeding. Isolates from 6 mice are seeded immediately after bleeding (direct seeding) and 6 after overnight storage at 4°C. Depending on the mouse, some isolates turned rapidly to gametocyte production (gam). No adjunction of RBC was done in the cultures. B/Follow up of parasitaemia after seeding of 18 separate isolates Isolates from 9 mice are seeded immediately after bleeding (direct seeding) and 9 after overnight storage of the infected blood at 4°C (ON). RBC are added after 2 days of culture, as marked by the dotted line (dilution 1:1 RBC:IRBC). After five days of culture most of the isolates seeded immediately after bleeding still harboured more than 30% of their initial parasitaemia (at the seeding day). After 12 days of culture parasitaemia of the isolates (direct seeding) are usually between 1 to 5% (data not shown). This is a different set of mice from Figure 2A.

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