Figure 1

Expression of VAR2CSA-DBL recombinant proteins in P. pastoris. A) Protein schematic of VAR2CSA. The original DBL domain boundaries are indicated by black rectangles. Revised domain boundaries as described [35] are indicated in grey and numbered for the 7G8-VAR2CSA allele. The first and last amino acids of constructs are indicated below the schematic. The thin line refers to the non-var2csa encoded protein IT4var22 DBL3. B) 1 μg of His-tagged recombinant proteins were analysed under non-reducing conditions in a 4-20% SDS-PAGE gel and stained by Gel Code Blue Reagent or detected by immunoblot via anti-His tag antibodies. New 7G8-VAR2CSA and IT4var22 DBL3 recombinant proteins generated for this study are shown. Other recombinant proteins were described previously [35].