Expression of VAR2CSA-DBL recombinant proteins in P. pastoris. A) Protein schematic of VAR2CSA. The original DBL domain boundaries are indicated by black rectangles. Revised domain boundaries as described  are indicated in grey and numbered for the 7G8-VAR2CSA allele. The first and last amino acids of constructs are indicated below the schematic. The thin line refers to the non-var2csa encoded protein IT4var22 DBL3. B) 1 μg of His-tagged recombinant proteins were analysed under non-reducing conditions in a 4-20% SDS-PAGE gel and stained by Gel Code Blue Reagent or detected by immunoblot via anti-His tag antibodies. New 7G8-VAR2CSA and IT4var22 DBL3 recombinant proteins generated for this study are shown. Other recombinant proteins were described previously .