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Table 1 Immunization protocol

From: Induction of strain-transcendent antibodies to placental-type isolates with VAR2CSA DBL3 or DBL5 recombinant proteins

Immunogen VAR2CSA Animal Number of injections1 Vaccination doses (μg)
     Prime 2 Boost 3
DBL1 7G8 3 rabbits 4 5 100 100
   3 rats 4 20 20
  IT4 2 rabbits 5 6 500 250
DBL3 7G8 3 rats 4 20 20
  IT4 3 rabbits 4 50 50
DBL4 short IT4 3 rats 4 20 20
   2 rabbits 5 6 500 250
DBL4 long 7G8 3 rats 4 20 20
  IT4 3 rats 4 20 20
DBL4+DBL5 7G8 3 rats 4 20+20 20+20
DBL5 7G8 3 rabbits 4 5 100 100
   3 rats 4 20 20
  IT4 3 rabbits 4 5 25 25
  3D7 3 rabbits 4 5 50 50
   3 rats 4 20 20
DBL6 7G8 3 rats 4 20 20
  IT4 2 rabbits 5 6 500 250
   3 rats 4 20 20
Immunogen control var22    
DBL3 IT4 3 rabbits 4 5 50 50
   3 rats 4 20 20
  1. 1. Animals were immunized subcutaneously
  2. 2. Recombinant protein in Complete Freund's adjuvant
  3. 3. Recombinant protein in Incomplete Freund's adjuvant
  4. 4. As previously published [30]
  5. 5. As previously published [29]